5uzz - Revision history

OCA at 12:15, 6 November 2024

2024-11-06T12:15:15Z

← Older revision		Revision as of 12:15, 6 November 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzz OCA], [https://pdbe.org/5uzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uzz RCSB], [https://www.ebi.ac.uk/pdbsum/5uzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzz OCA], [https://pdbe.org/5uzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uzz RCSB], [https://www.ebi.ac.uk/pdbsum/5uzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzz ProSAT]</span></td></tr>
	</table>		</table>

OCA at 10:27, 14 June 2023

2023-06-14T10:27:41Z

← Older revision		Revision as of 10:27, 14 June 2023
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	==Structure of wild type pre-miR21 apical loop==		==Structure of wild type pre-miR21 apical loop==
	<StructureSection load='5uzz' size='340' side='right'caption='[[5uzz~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>		<StructureSection load='5uzz' size='340' side='right'caption='[[5uzz]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene>~~</td></tr>~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uzt\|5uzt]], [[5v29\|5v29]]~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzz OCA], [https://pdbe.org/5uzz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uzz RCSB], [https://www.ebi.ac.uk/pdbsum/5uzz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzz ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzz OCA], [~~http~~://pdbe.org/5uzz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5uzz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5uzz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzz ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Shortridge, M D~~]]		[[Category: Synthetic construct]]
	[[Category: ~~Varani, G~~]]		[[Category: Shortridge MD]]
	[[Category: ~~Microrna mir-21 oncomir pre-mirna]]~~		[[Category: Varani G]]
	~~[[Category: Transport protein-rna complex~~]]

OCA at 09:25, 1 January 2020

2020-01-01T09:25:21Z

← Older revision		Revision as of 09:25, 1 January 2020
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	==Structure of wild type pre-miR21 apical loop==		==Structure of wild type pre-miR21 apical loop==
	<StructureSection load='5uzz' size='340' side='right' caption='[[5uzz]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>		<StructureSection load='5uzz' size='340' side='right'caption='[[5uzz]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Shortridge, M D]]		[[Category: Shortridge, M D]]
	[[Category: Varani, G]]		[[Category: Varani, G]]
	[[Category: Microrna mir-21 oncomir pre-mirna]]		[[Category: Microrna mir-21 oncomir pre-mirna]]
	[[Category: Transport protein-rna complex]]		[[Category: Transport protein-rna complex]]

OCA at 10:33, 3 August 2017

2017-08-03T10:33:34Z

← Older revision		Revision as of 10:33, 3 August 2017
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	~~'''Unreleased structure'''~~

	The ~~entry 5uzz~~ is ~~ON HOLD~~		==Structure of wild type pre-miR21 apical loop==
			<StructureSection load='5uzz' size='340' side='right' caption='[[5uzz]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5uzz]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UZZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UZZ FirstGlance]. <br>
			</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DPR:D-PROLINE'>DPR</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5uzt\|5uzt]], [[5v29\|5v29]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uzz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uzz OCA], [http://pdbe.org/5uzz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uzz RCSB], [http://www.ebi.ac.uk/pdbsum/5uzz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uzz ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			MicroRNAs (miRNAs) help orchestrate cellular growth and survival through post-transcriptional mechanisms. The dysregulation of miRNA biogenesis can lead to cellular growth defects and chemotherapeutic resistance and plays a direct role in the development of many chronic diseases. Among these RNAs, miR-21 is consistently overexpressed in most human cancers, leading to the down-regulation of key tumor-suppressing and pro-apoptotic factors, suggesting that inhibition of miR-21 biogenesis could reverse these negative effects. However, targeted inhibition of miR-21 using small molecules has had limited success. To overcome difficulties in targeting RNA secondary structure with small molecules, we developed a class of cyclic beta-hairpin peptidomimetics which bind to RNA stem-loop structures, such as miRNA precursors, with potent affinity and specificity. We screened an existing cyclic peptide library and discovered a lead structure which binds to pre-miR21 with KD = 200 nM and prefers it over other pre-miRNAs. The NMR structure of the complex shows that the peptide recognizes the Dicer cleavage site and alters processing of the precursor to the mature miRNA in vitro and in cultured cells. The structure provides a rationale for the peptide binding activity and clear guidance for further improvements in affinity and targeting.

	~~Authors:~~ Shortridge, ~~M.D.~~, Varani, G.		A Macrocyclic Peptide Ligand Binds the Oncogenic MicroRNA-21 Precursor and Suppresses Dicer Processing.,Shortridge MD, Walker MJ, Pavelitz T, Chen Y, Yang W, Varani G ACS Chem Biol. 2017 May 2. doi: 10.1021/acschembio.7b00180. PMID:28437065<ref>PMID:28437065</ref>

	~~Description: Structure~~ of ~~wild type pre~~-~~miR21 apical loop~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
			<div class="pdbe-citations 5uzz" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Shortridge, M D]]
	[[Category: Varani, G]]		[[Category: Varani, G]]
	[[Category: ~~Shortridge, M.D~~]]		[[Category: Microrna mir-21 oncomir pre-mirna]]
			[[Category: Transport protein-rna complex]]

OCA at 11:28, 5 April 2017

2017-04-05T11:28:24Z

← Older revision		Revision as of 11:28, 5 April 2017
Line 3:		Line 3:
	The entry 5uzz is ON HOLD		The entry 5uzz is ON HOLD

	Authors: Shortridge, M.D., Varani, G		Authors: Shortridge, M.D., Varani, G.

	Description: Structure of wild type pre-miR21 apical loop		Description: Structure of wild type pre-miR21 apical loop

OCA: Protected "5uzz" [edit=sysop:move=sysop]

2017-03-09T15:09:53Z

Protected "5uzz" [edit=sysop:move=sysop]

← Older revision	Revision as of 15:09, 9 March 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5uzz is ON HOLD Authors: Shortridge, M.D., Varani, G Description: Structure of wild type pre-miR21 apical loop Category: Unreleased Structures ...

2017-03-09T15:09:51Z

New page: '''Unreleased structure''' The entry 5uzz is ON HOLD Authors: Shortridge, M.D., Varani, G Description: Structure of wild type pre-miR21 apical loop Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 5uzz is ON HOLD

Authors: Shortridge, M.D., Varani, G

Description: Structure of wild type pre-miR21 apical loop
[[Category: Unreleased Structures]]
[[Category: Varani, G]]
[[Category: Shortridge, M.D]]