5vae - Revision history

OCA at 09:30, 23 October 2024

2024-10-23T09:30:21Z

← Older revision		Revision as of 09:30, 23 October 2024
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	<StructureSection load='5vae' size='340' side='right'caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='5vae' size='340' side='right'caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_10558 Atcc 10558~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_gordonii Streptococcus gordonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VAE FirstGlance]. <br>
	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.106Å</td></tr>
	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5vaf\|5vaf~~]]</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~asp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=1302 ATCC 10558]), asp3 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vae OCA], [https://pdbe.org/5vae PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vae RCSB], [https://www.ebi.ac.uk/pdbsum/5vae PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vae ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vae OCA], [~~http~~://pdbe.org/5vae PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vae RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vae PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vae ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ASP1_STRGN ASP1_STRGN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Atcc 10558]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Chen, Y~~]]		[[Category: Streptococcus gordonii]]
	[[Category: ~~Jeffrey, P D~~]]		[[Category: Chen Y]]
	[[Category: ~~Rapoport, T A~~]]		[[Category: Jeffrey PD]]
	[[Category: ~~Accessory secretion]]~~		[[Category: Rapoport TA]]
	~~[[Category: Bacterial adhesin]]~~
	~~[[Category: Cell adhesion]]~~
	~~[[Category: O-glycosylation~~]]

OCA at 16:54, 20 November 2019

2019-11-20T16:54:55Z

← Older revision		Revision as of 16:54, 20 November 2019
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	==Crystal structure of accessory secretion protein 1 and 3==		==Crystal structure of accessory secretion protein 1 and 3==
	<StructureSection load='5vae' size='340' side='right' caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='5vae' size='340' side='right'caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAE FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 10558]]		[[Category: Atcc 10558]]
			[[Category: Large Structures]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Jeffrey, P D]]		[[Category: Jeffrey, P D]]

OCA at 07:10, 28 February 2018

2018-02-28T07:10:57Z

← Older revision		Revision as of 07:10, 28 February 2018
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	<StructureSection load='5vae' size='340' side='right' caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>		<StructureSection load='5vae' size='340' side='right' caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAE FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10558 Atcc 10558]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAE FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vaf\|5vaf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vaf\|5vaf]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">asp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558]), asp3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1302 ATCC 10558])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vae OCA], [http://pdbe.org/5vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vae RCSB], [http://www.ebi.ac.uk/pdbsum/5vae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vae ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vae OCA], [http://pdbe.org/5vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vae RCSB], [http://www.ebi.ac.uk/pdbsum/5vae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vae ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Many pathogenic bacteria, including Streptococcus gordonii, possess a pathway for the cellular export of a single serine-rich-repeat protein that mediates the adhesion of bacteria to host cells and the extracellular matrix. This adhesin protein is O-glycosylated by several cytosolic glycosyltransferases and requires three accessory Sec proteins (Asp1-3) for export, but how the adhesin protein is processed for export is not well understood. Here, we report that the S. gordonii adhesin GspB is sequentially O-glycosylated by three enzymes (GtfA/B, Nss, and Gly) that attach N-acetylglucosamine and glucose to Ser/Thr residues. We also found that modified GspB is transferred from the last glycosyltransferase to the Asp1/2/3 complex. Crystal structures revealed that both Asp1 and Asp3 are related to carbohydrate-binding proteins, suggesting that they interact with carbohydrates and bind glycosylated adhesin, a notion that was supported by further analyses. We further observed that Asp1 also has an affinity for phospholipids, which is attenuated by Asp2. In summary, our findings support a model in which the GspB adhesin is sequentially glycosylated by GtfA/B, Nss, and Gly and then transferred to the Asp1/2/3 complex in which Asp1 mediates the interaction of the Asp1/2/3 complex with the lipid bilayer for targeting of matured GspB to the export machinery.

			Unraveling the sequence of cytosolic reactions in the export of GspB adhesin from Streptococcus gordonii.,Chen Y, Bensing BA, Seepersaud R, Mi W, Liao M, Jeffrey PD, Shajahan A, Sonon RN, Azadi P, Sullam PM, Rapoport TA J Biol Chem. 2018 Feb 9. pii: RA117.000963. doi: 10.1074/jbc.RA117.000963. PMID:29462788<ref>PMID:29462788</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 5vae" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Atcc 10558]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Jeffrey, P D]]		[[Category: Jeffrey, P D]]

OCA at 07:15, 22 February 2018

2018-02-22T07:15:57Z

← Older revision		Revision as of 07:15, 22 February 2018
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5vae is ~~ON HOLD until Paper Publication~~		==Crystal structure of accessory secretion protein 1 and 3==
			<StructureSection load='5vae' size='340' side='right' caption='[[5vae]], [[Resolution\|resolution]] 3.11Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[5vae]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAE FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5vaf\|5vaf]]</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vae FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vae OCA], [http://pdbe.org/5vae PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vae RCSB], [http://www.ebi.ac.uk/pdbsum/5vae PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vae ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Chen, Y]]
			[[Category: Jeffrey, P D]]
			[[Category: Rapoport, T A]]
			[[Category: Accessory secretion]]
			[[Category: Bacterial adhesin]]
			[[Category: Cell adhesion]]
			[[Category: O-glycosylation]]

OCA at 11:13, 19 April 2017

2017-04-19T11:13:57Z

← Older revision		Revision as of 11:13, 19 April 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5vae is ON HOLD		The entry 5vae is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5vae" [edit=sysop:move=sysop]

2017-04-05T11:51:32Z

Protected "5vae" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:51, 5 April 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5vae is ON HOLD Authors: Description: Category: Unreleased Structures

2017-04-05T11:51:31Z

New page: '''Unreleased structure''' The entry 5vae is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5vae is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]