5vbk - Revision history

OCA at 13:45, 4 October 2023

2023-10-04T13:45:23Z

← Older revision		Revision as of 13:45, 4 October 2023
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	<StructureSection load='5vbk' size='340' side='right'caption='[[5vbk]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='5vbk' size='340' side='right'caption='[[5vbk]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vbk]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Mytilus_californianus Mytilus californianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VBK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5VBK FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vbk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mytilus_californianus Mytilus californianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VBK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.788Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vbk OCA], [~~http~~://pdbe.org/5vbk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5vbk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5vbk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5vbk ProSAT]</span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vbk OCA], [https://pdbe.org/5vbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vbk RCSB], [https://www.ebi.ac.uk/pdbsum/5vbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vbk ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/LEC_MYTCA LEC_MYTCA]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 5vbk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5vbk" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Galactose-binding lectin\|Galactose-binding lectin]]
	== References ==		== References ==
	<references/>		<references/>
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Mytilus californianus]]		[[Category: Mytilus californianus]]
	[[Category: Garcia-Maldonado, E]]		[[Category: Garcia-Maldonado E]]
	[[Category: Hernandez-Santoyo, A]]		[[Category: Hernandez-Santoyo A]]
	~~[[Category: Galactose-binding lectin]]~~
	~~[[Category: Sugar binding protein~~]]

OCA at 08:34, 30 September 2020

2020-09-30T08:34:30Z

← Older revision		Revision as of 08:34, 30 September 2020
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	==Crystal structure of a galactose-binding Lectin from Mytilus californianus==		==Crystal structure of a galactose-binding Lectin from Mytilus californianus==
	<StructureSection load='5vbk' size='340' side='right' caption='[[5vbk]], [[Resolution\|resolution]] 1.79Å' scene=''>		<StructureSection load='5vbk' size='340' side='right'caption='[[5vbk]], [[Resolution\|resolution]] 1.79Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5vbk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mytilus_californianus Mytilus californianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VBK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5VBK FirstGlance]. <br>		<table><tr><td colspan='2'>[[5vbk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mytilus_californianus Mytilus californianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VBK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5VBK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vbk OCA], [http://pdbe.org/5vbk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vbk RCSB], [http://www.ebi.ac.uk/pdbsum/5vbk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vbk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vbk OCA], [http://pdbe.org/5vbk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vbk RCSB], [http://www.ebi.ac.uk/pdbsum/5vbk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vbk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Mytilus californianus]]		[[Category: Mytilus californianus]]
	[[Category: Garcia-Maldonado, E]]		[[Category: Garcia-Maldonado, E]]

OCA at 10:58, 16 November 2017

2017-11-16T10:58:42Z

← Older revision		Revision as of 10:58, 16 November 2017
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	~~'''Unreleased structure'''~~

	~~The entry~~ 5vbk is ~~ON HOLD until Paper~~ Publication		==Crystal structure of a galactose-binding Lectin from Mytilus californianus==
			<StructureSection load='5vbk' size='340' side='right' caption='[[5vbk]], [[Resolution\|resolution]] 1.79Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[5vbk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mytilus_californianus Mytilus californianus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VBK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VBK FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vbk OCA], [http://pdbe.org/5vbk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vbk RCSB], [http://www.ebi.ac.uk/pdbsum/5vbk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vbk ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Lectins play crucial roles for innate immune responses in invertebrates by recognizing and eliminating pathogens. In this study, a lectin from the mussel Mytilus californianus (MCL) was identified and characterized. The lectin was purified by affinity chromatography in alpha-lactose-agarose resin showing an experimental molecular mass of 18000 Da as determined by SDS-PAGE and MALDI-TOF mass spectrometry. It was specific for binding d-galactose and N-Acetyl-d-galactosamine that contained carbohydrate moieties that were also inhibited by melibiose and raffinose. It had the ability to agglutinate all types of human erythrocytes, as well as rabbit red blood cells. Circular dichroism analyzes have indicated that this lectin possessed an alpha/beta fold with a predominance of beta structures. This was consistent with the structure of the protein that was determined by the X-ray diffraction techniques. MCL was crystallized in the space group C21 and it diffracted to 1.79 A resolution. Two monomers were found in the asymmetric unit and they formed dimers in solution. The protein has shown to be a member of the beta-trefoil family, with three sugar binding sites per monomer. In accord with fluorescence-based thermal shift assays, we observed that the MCL Tm increased about 10 degrees C in the presence of galactose. Furthermore, we have determined the complete amino acid sequence by cDNA sequencing. The gene had two ORF2 proteins, one resulting in a 180 residue protein with a theoretical molecular mass of 20227 Da, and another resulting in a 150 residue protein with a theoretical molecular mass of 16911 Da. The difference between the theoretical and experimental values was due to the presence of a glycosylation that was observed by the glycosylation assay. A positive microbial agglutination and a growth inhibition activity were observed against Gram-negative and Gram-positive bacteria. The M. californianus lectin is the fourth member of the recently proposed new family of lectins that have been reported to date, occurring only in mollusks belonging to the family Mytilidae. It is the first member to be glycosylated and with a strong tendency to form large oligomers.

	~~Authors:~~ Hernandez-Santoyo, A.~~, Garcia~~-~~Maldonado~~, E.		Molecular and functional characterization of a glycosylated Galactose-Binding lectin from Mytilus californianus.,Garcia-Maldonado E, Cano-Sanchez P, Hernandez-Santoyo A Fish Shellfish Immunol. 2017 Jul;66:564-574. doi: 10.1016/j.fsi.2017.05.057. Epub, 2017 May 22. PMID:28546025<ref>PMID:28546025</ref>

	~~Description: Crystal structure~~ of ~~a galactose~~-~~binding Lectin from~~ Mytilus californianus		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
			<div class="pdbe-citations 5vbk" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Mytilus californianus]]
			[[Category: Garcia-Maldonado, E]]
	[[Category: Hernandez-Santoyo, A]]		[[Category: Hernandez-Santoyo, A]]
	[[Category: ~~Garcia~~-~~Maldonado, E~~]]		[[Category: Galactose-binding lectin]]
			[[Category: Sugar binding protein]]

OCA at 11:15, 19 April 2017

2017-04-19T11:15:37Z

← Older revision		Revision as of 11:15, 19 April 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 5vbk is ON HOLD		The entry 5vbk is ON HOLD until Paper Publication

	Authors: Hernandez-Santoyo, A., Garcia-Maldonado, E.		Authors: Hernandez-Santoyo, A., Garcia-Maldonado, E.

OCA: Protected "5vbk" [edit=sysop:move=sysop]

2017-04-05T11:53:44Z

Protected "5vbk" [edit=sysop:move=sysop]

← Older revision	Revision as of 11:53, 5 April 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 5vbk is ON HOLD Authors: Hernandez-Santoyo, A., Garcia-Maldonado, E. Description: Crystal structure of a galactose-binding Lectin from Mytilus cali...

2017-04-05T11:53:43Z

New page: '''Unreleased structure''' The entry 5vbk is ON HOLD Authors: Hernandez-Santoyo, A., Garcia-Maldonado, E. Description: Crystal structure of a galactose-binding Lectin from Mytilus cali...

New page

'''Unreleased structure'''

The entry 5vbk is ON HOLD

Authors: Hernandez-Santoyo, A., Garcia-Maldonado, E.

Description: Crystal structure of a galactose-binding Lectin from Mytilus californianus
[[Category: Unreleased Structures]]
[[Category: Hernandez-Santoyo, A]]
[[Category: Garcia-Maldonado, E]]