6bme - Revision history

OCA at 13:02, 13 August 2026

2026-08-13T13:02:21Z

← Older revision		Revision as of 13:02, 13 August 2026
Line 10:		Line 10:
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/R9RY64_CHLRE R9RY64_CHLRE]		[https://www.uniprot.org/uniprot/R9RY64_CHLRE R9RY64_CHLRE]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			BACKGROUND: The nuclear genome of Chlamydomonas reinhardtii encodes a dozen hemoglobins of the truncated lineage. Four of these, named THB1-4, contain a single ~130-residue globin unit. THB1, which is cytoplasmic and capable of nitric oxide dioxygenation activity, uses a histidine and a lysine as axial ligands to the heme iron. In the present report, we compared THB2, THB3, and THB4 to THB1 to gain structural and functional insights into algal globins. METHODS: We inspected properties of the globin domains prepared by recombinant means through site-directed mutagenesis, electronic absorption, CD, and NMR spectroscopies, and X-ray crystallography. RESULTS: Recombinant THB3, which lacks the proximal histidine but has a distal histidine, binds heme weakly. NMR data demonstrate that the recombinant domains of THB2 and THB4 coordinate the ferrous heme iron with the proximal histidine and a lysine from the distal helix. An X-ray structure of ferric THB4 confirms lysine coordination. THB1, THB2, and THB4 have reduction potentials between -65 and -100 mV, are capable of nitric oxide dioxygenation, are reduced at different rates by the diaphorase domain of C. reinhardtii nitrate reductase, and show different response to peroxide treatment. CONCLUSIONS: Three single-domain C. reinhardtii hemoglobins use lysine as a distal heme ligand in both Fe(III) and Fe(II) oxidation states. This common feature is likely related to enzymatic activity in the management of reactive oxygen species. GENERAL SIGNIFICANCE: Primary structure analysis of hemoglobins has limited power in the prediction of heme ligation. Experimental determination reveals variations in this essential property across the superfamily.

			Lysine as a heme iron ligand: A property common to three truncated hemoglobins from Chlamydomonas reinhardtii.,Johnson EA, Russo MM, Nye DB, Schlessman JL, Lecomte JTJ Biochim Biophys Acta Gen Subj. 2018 Dec;1862(12):2660-2673. doi:, 10.1016/j.bbagen.2018.08.009. Epub 2018 Aug 10. PMID:30251657<ref>PMID:30251657</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6bme" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]		*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:22, 13 March 2024

2024-03-13T14:22:36Z

← Older revision		Revision as of 14:22, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6bme' size='340' side='right'caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6bme' size='340' side='right'caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chlre Chlre~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BME FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene~~>, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.899Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~THB4 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=3055 CHLRE])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [~~http~~://pdbe.org/6bme PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6bme PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [https://pdbe.org/6bme PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [https://www.ebi.ac.uk/pdbsum/6bme PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/R9RY64_CHLRE R9RY64_CHLRE]
	BACKGROUND: The nuclear genome of Chlamydomonas reinhardtii encodes a dozen hemoglobins of the truncated lineage. Four of these, named THB1-4, contain a single ~130-residue globin unit. THB1, which is cytoplasmic and capable of nitric oxide dioxygenation activity, uses a histidine and a lysine as axial ligands to the heme iron. In the present report, we compared THB2, THB3, and THB4 to THB1 to gain structural and functional insights into algal globins. METHODS: We inspected properties of the globin domains prepared by recombinant means through site-directed mutagenesis, electronic absorption, CD, and NMR spectroscopies, and X-ray crystallography. RESULTS: Recombinant THB3, which lacks the proximal histidine but has a distal histidine, binds heme weakly. NMR data demonstrate that the recombinant domains of THB2 and THB4 coordinate the ferrous heme iron with the proximal histidine and a lysine from the distal helix. An X-ray structure of ferric THB4 confirms lysine coordination. THB1, THB2, and THB4 have reduction potentials between -65 and -100 mV, are capable of nitric oxide dioxygenation, are reduced at different rates by the diaphorase domain of C. reinhardtii nitrate reductase, and show different response to peroxide treatment. CONCLUSIONS: Three single-domain C. reinhardtii hemoglobins use lysine as a distal heme ligand in both Fe(III) and Fe(II) oxidation states. This common feature is likely related to enzymatic activity in the management of reactive oxygen species. GENERAL SIGNIFICANCE: Primary structure analysis of hemoglobins has limited power in the prediction of heme ligation. Experimental determination reveals variations in this essential property across the superfamily.

	Lysine as a heme iron ligand: A property common to three truncated hemoglobins from Chlamydomonas reinhardtii.,Johnson EA, Russo MM, Nye DB, Schlessman JL, Lecomte JTJ Biochim Biophys Acta Gen Subj. 2018 Dec;1862(12):2660-2673. doi:, 10.1016/j.bbagen.2018.08.009. Epub 2018 Aug 10. PMID:30251657<ref>PMID:~~30251657<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U.S~~. ~~National Library of Medicine~~.~~<br>~~
	</~~div>~~
	~~<div class="pdbe-citations 6bme" style="background-color:#fffaf0;"><~~/~~div>~~

	==See Also==		==See Also==
	*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]		*[[Hemoglobin 3D structures\|Hemoglobin 3D structures]]
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Chlre~~]]		[[Category: Chlamydomonas reinhardtii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lecomte~~, J T.J~~]]		[[Category: Lecomte JTJ]]
	[[Category: Russo~~, M M~~]]		[[Category: Russo MM]]
	[[Category: Schlessman~~, J L]]~~		[[Category: Schlessman JL]]
	~~[[Category: 2-on-2 hemoglobin]]~~
	~~[[Category: Globin]]~~
	~~[[Category: Heme]]~~
	~~[[Category: Heme binding protein]]~~
	~~[[Category: Hexacoordinate]]~~
	~~[[Category: Lysine axial ligand]]~~
	~~[[Category: Truncated hemoglobin~~]]

OCA at 08:19, 27 November 2019

2019-11-27T08:19:40Z

← Older revision		Revision as of 08:19, 27 November 2019
Line 1:		Line 1:

	==Crystal structure of Chlamydomonas reinhardtii THB4==		==Crystal structure of Chlamydomonas reinhardtii THB4==
	<StructureSection load='6bme' size='340' side='right' caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6bme' size='340' side='right'caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BME FirstGlance]. <br>
Line 25:		Line 25:
	</StructureSection>		</StructureSection>
	[[Category: Chlre]]		[[Category: Chlre]]
			[[Category: Large Structures]]
	[[Category: Lecomte, J T.J]]		[[Category: Lecomte, J T.J]]
	[[Category: Russo, M M]]		[[Category: Russo, M M]]

OCA at 12:29, 16 January 2019

2019-01-16T12:29:04Z

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [http://pdbe.org/6bme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [http://www.ebi.ac.uk/pdbsum/6bme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>
 </table>
 __TOC__
 </StructureSection>

OCA at 08:26, 17 January 2018

2018-01-17T08:26:44Z

← Older revision		Revision as of 08:26, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='6bme' size='340' side='right' caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6bme' size='340' side='right' caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BME FirstGlance]. <br>		<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BME FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">THB4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [http://pdbe.org/6bme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [http://www.ebi.ac.uk/pdbsum/6bme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [http://pdbe.org/6bme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [http://www.ebi.ac.uk/pdbsum/6bme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Chlre]]
	[[Category: Lecomte, J T.J]]		[[Category: Lecomte, J T.J]]
	[[Category: Russo, M M]]		[[Category: Russo, M M]]

OCA at 06:24, 20 December 2017

2017-12-20T06:24:43Z

← Older revision		Revision as of 06:24, 20 December 2017
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 6bme is ~~ON HOLD~~		==Crystal structure of Chlamydomonas reinhardtii THB4==
			<StructureSection load='6bme' size='340' side='right' caption='[[6bme]], [[Resolution\|resolution]] 1.90Å' scene=''>
	~~Authors~~: ~~Russo~~, M.M., ~~Schlessman~~, J.L., ~~Lecomte~~, J.~~T.J~~.		== Structural highlights ==
			<table><tr><td colspan='2'>[[6bme]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6BME FirstGlance]. <br>
	~~Description: Crystal structure of Chlamydomonas reinhardtii THB4~~		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6bme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bme OCA], [http://pdbe.org/6bme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6bme RCSB], [http://www.ebi.ac.uk/pdbsum/6bme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6bme ProSAT]</span></td></tr>
	[[Category: Russo, M.M]]		</table>
	[[Category: ~~Lecomte~~, J~~.T.J~~]]		__TOC__
	[[Category: ~~Schlessman, J.L~~]]		</StructureSection>
			[[Category: Lecomte, J T.J]]
			[[Category: Russo, M M]]
			[[Category: Schlessman, J L]]
			[[Category: 2-on-2 hemoglobin]]
			[[Category: Globin]]
			[[Category: Heme]]
			[[Category: Heme binding protein]]
			[[Category: Hexacoordinate]]
			[[Category: Lysine axial ligand]]
			[[Category: Truncated hemoglobin]]

OCA: Protected "6bme" [edit=sysop:move=sysop]

2017-11-22T06:36:22Z

Protected "6bme" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:36, 22 November 2017
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6bme is ON HOLD Authors: Russo, M.M., Schlessman, J.L., Lecomte, J.T.J. Description: Crystal structure of Chlamydomonas reinhardtii THB4 [[Category...

2017-11-22T06:36:21Z

New page: '''Unreleased structure''' The entry 6bme is ON HOLD Authors: Russo, M.M., Schlessman, J.L., Lecomte, J.T.J. Description: Crystal structure of Chlamydomonas reinhardtii THB4 [[Category...

New page

'''Unreleased structure'''

The entry 6bme is ON HOLD

Authors: Russo, M.M., Schlessman, J.L., Lecomte, J.T.J.

Description: Crystal structure of Chlamydomonas reinhardtii THB4
[[Category: Unreleased Structures]]
[[Category: Russo, M.M]]
[[Category: Lecomte, J.T.J]]
[[Category: Schlessman, J.L]]