6cs2 - Revision history

OCA at 07:52, 17 October 2024

2024-10-17T07:52:31Z

← Older revision		Revision as of 07:52, 17 October 2024
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	<SX load='6cs2' size='340' side='right' viewer='molstar' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6cs2' size='340' side='right' viewer='molstar' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bpt4 Bpt4~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome-related_coronavirus Severe acute respiratory syndrome-related coronavirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>~~, ~~<scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.4Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=10665 BPT4]), ACE2, UNQ868/PRO1885 ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td~~></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[http~~:~~//en.wikipedia.org/wiki/Angiotensin~~-~~converting_enzyme_2 Angiotensin~~-~~converting enzyme 2], with EC number [http://www.brenda~~-~~enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23]~~ </~~span~~></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [https://pdbe.org/6cs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [https://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [~~http~~://pdbe.org/6cs2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[~~http~~://www.uniprot.org/uniprot/~~SPIKE_CVHSA SPIKE_CVHSA~~]~~] S1 attaches~~ the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR~~, initiating the infection. Binding to the receptor~~ and internalization of the virus into the endosomes of the host cell ~~probably~~ induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate ~~membranes~~ fusion within endosomes. ~~S2 is~~ a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [~~[http~~:~~//www.uniprot.org/uniprot/ACE2_HUMAN ACE2_HUMAN~~]~~] Carboxypeptidase which converts angiotensin I to angiotensin 1-9,~~ a peptide ~~of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator~~. ~~Also able to hydrolyze apelin-13 and dynorphin~~-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.<ref>PMID:~~10969042</ref>~~ <ref>PMID:~~10924499~~</ref> ~~<ref>PMID:14647384</ref>~~		[https://www.uniprot.org/uniprot/WAC_BPT4 WAC_BPT4] Chaperone responsible for attachment of long tail fibers to virus particle. Forms the fibrous structure on the neck of the virion called whiskers. During phage assembly, 6 fibritin molecules attach to each virion neck through their N-terminal domains, to form a collar with six fibers ('whiskers').[https://www.uniprot.org/uniprot/SPIKE_SARS SPIKE_SARS] May down-regulate host tetherin (BST2) by lysosomal degradation, thereby counteracting its antiviral activity.<ref>PMID:31199522</ref> Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membrane fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:14670965</ref> <ref>PMID:15496474</ref> Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]<ref>PMID:19321428</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]		*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]
	*[[Fibritin\|Fibritin]]		*[[Fibritin\|Fibritin]]
	*[[Spike protein\|Spike protein]]		*[[Sandbox 3001\|Sandbox 3001]]
			*[[Spike protein 3D structures\|Spike protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: ~~Angiotensin-converting enzyme 2~~]]		[[Category: Escherichia virus T4]]
	[[Category: ~~Bpt4]]~~		[[Category: Homo sapiens]]
	~~[[Category: Human~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Cottrell~~, C A~~]]		[[Category: Severe acute respiratory syndrome-related coronavirus]]
	[[Category: Kirchdoerfer~~, R N~~]]		[[Category: Cottrell CA]]
	[[Category: McLellan~~, J S~~]]		[[Category: Kirchdoerfer RN]]
	[[Category: Pallesen, J]]		[[Category: McLellan JS]]
	[[Category: Turner~~, H L~~]]		[[Category: Pallesen J]]
	[[Category: Wang, N]]		[[Category: Turner HL]]
	[[Category: Ward~~, A B]]~~		[[Category: Wang N]]
	~~[[Category: Glycoprotein]]~~		[[Category: Ward AB]]
	~~[[Category: Membrane fusion]]~~
	~~[[Category: Receptor binding]]~~
	~~[[Category: Viral protein]]~~
	~~[[Category: Viral protein-hydrolase complex~~]]

OCA at 23:49, 10 April 2020

2020-04-10T23:49:43Z

← Older revision		Revision as of 23:49, 10 April 2020
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	<SX load='6cs2' size='340' side='right' viewer='molstar' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6cs2' size='340' side='right' viewer='molstar' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), ACE2, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), ACE2, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]		*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]
	*[[Fibritin\|Fibritin]]		*[[Fibritin\|Fibritin]]
			*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 20:48, 6 March 2020

2020-03-06T20:48:09Z

← Older revision		Revision as of 20:48, 6 March 2020
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	==SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles==		==SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles==
	<~~StructureSection~~ load='6cs2' size='340' side='right'caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6cs2' size='340' side='right' viewer='molstar' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
	</~~StructureSection~~>		</SX>
	[[Category: Angiotensin-converting enzyme 2]]		[[Category: Angiotensin-converting enzyme 2]]
	[[Category: Bpt4]]		[[Category: Bpt4]]

OCA at 09:50, 18 December 2019

2019-12-18T09:50:14Z

← Older revision		Revision as of 09:50, 18 December 2019
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	==SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles==		==SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles==
	<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='6cs2' size='340' side='right'caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
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	==See Also==		==See Also==
			*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]
	*[[Fibritin\|Fibritin]]		*[[Fibritin\|Fibritin]]
	== References ==		== References ==
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	[[Category: Bpt4]]		[[Category: Bpt4]]
	[[Category: Human]]		[[Category: Human]]
			[[Category: Large Structures]]
	[[Category: Cottrell, C A]]		[[Category: Cottrell, C A]]
	[[Category: Kirchdoerfer, R N]]		[[Category: Kirchdoerfer, R N]]

OCA at 07:13, 12 December 2018

2018-12-12T07:13:40Z

← Older revision		Revision as of 07:13, 12 December 2018
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	<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~9hiv1 9hiv1~~] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpt4 Bpt4] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~11676 9HIV1~~]), ACE2, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10665 BPT4]), ACE2, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [[http://www.uniprot.org/uniprot/ACE2_HUMAN ACE2_HUMAN]] Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.<ref>PMID:10969042</ref> <ref>PMID:10924499</ref> <ref>PMID:14647384</ref>		[[http://www.uniprot.org/uniprot/SPIKE_CVHSA SPIKE_CVHSA]] S1 attaches the virion to the cell membrane by interacting with human ACE2 and CLEC4M/DC-SIGNR, initiating the infection. Binding to the receptor and internalization of the virus into the endosomes of the host cell probably induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. S2 is a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. [[http://www.uniprot.org/uniprot/ACE2_HUMAN ACE2_HUMAN]] Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.<ref>PMID:10969042</ref> <ref>PMID:10924499</ref> <ref>PMID:14647384</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Severe acute respiratory syndrome coronavirus (SARS-CoV) emerged in 2002 as a highly transmissible pathogenic human betacoronavirus. The viral spike glycoprotein (S) utilizes angiotensin-converting enzyme 2 (ACE2) as a host protein receptor and mediates fusion of the viral and host membranes, making S essential to viral entry into host cells and host species tropism. As SARS-CoV enters host cells, the viral S is believed to undergo a number of conformational transitions as it is cleaved by host proteases and binds to host receptors. We recently developed stabilizing mutations for coronavirus spikes that prevent the transition from the pre-fusion to post-fusion states. Here, we present cryo-EM analyses of a stabilized trimeric SARS-CoV S, as well as the trypsin-cleaved, stabilized S, and its interactions with ACE2. Neither binding to ACE2 nor cleavage by trypsin at the S1/S2 cleavage site impart large conformational changes within stabilized SARS-CoV S or expose the secondary cleavage site, S2'.

			Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis.,Kirchdoerfer RN, Wang N, Pallesen J, Wrapp D, Turner HL, Cottrell CA, Corbett KS, Graham BS, McLellan JS, Ward AB Sci Rep. 2018 Oct 24;8(1):15701. doi: 10.1038/s41598-018-34171-7. PMID:30356097<ref>PMID:30356097</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6cs2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Fibritin\|Fibritin]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Angiotensin-converting enzyme 2]]		[[Category: Angiotensin-converting enzyme 2]]
			[[Category: Bpt4]]
	[[Category: Human]]		[[Category: Human]]
	[[Category: Cottrell, C A]]		[[Category: Cottrell, C A]]

OCA at 06:07, 25 April 2018

2018-04-25T06:07:09Z

← Older revision		Revision as of 06:07, 25 April 2018
Line 3:		Line 3:
	<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>		<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/9hiv1 9hiv1] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">wac, T4Tp161 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=11676 9HIV1]), ACE2, UNQ868/PRO1885 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cs2 OCA], [http://pdbe.org/6cs2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cs2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cs2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cs2 ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Angiotensin-converting enzyme 2]]		[[Category: Angiotensin-converting enzyme 2]]
			[[Category: Human]]
	[[Category: Cottrell, C A]]		[[Category: Cottrell, C A]]
	[[Category: Kirchdoerfer, R N]]		[[Category: Kirchdoerfer, R N]]
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	[[Category: Receptor binding]]		[[Category: Receptor binding]]
	[[Category: Viral protein]]		[[Category: Viral protein]]
			[[Category: Viral protein-hydrolase complex]]

OCA at 13:55, 11 April 2018

2018-04-11T13:55:06Z

@@ Line 1: / Line 1: @@
-The entry 6cs2 is ON HOLD
+==SARS Spike Glycoprotein - human ACE2 complex, Stabilized variant, all ACE2-bound particles==
+<StructureSection load='6cs2' size='340' side='right' caption='[[6cs2]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6cs2]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CS2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CS2 FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Angiotensin-converting_enzyme_2 Angiotensin-converting enzyme 2], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.17.23 3.4.17.23] </span></td></tr>

OCA: Protected "6cs2" [edit=sysop:move=sysop]

2018-03-28T05:20:24Z

Protected "6cs2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:20, 28 March 2018
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6cs2 is ON HOLD Authors: Description: Category: Unreleased Structures

2018-03-28T05:20:23Z

New page: '''Unreleased structure''' The entry 6cs2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6cs2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]