6ect - Revision history

OCA at 14:40, 13 March 2024

2024-03-13T14:40:39Z

← Older revision		Revision as of 14:40, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6ect' size='340' side='right'caption='[[6ect]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='6ect' size='340' side='right'caption='[[6ect]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ect]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"chondromyces_aurantiacus"_(berkeley_and_curtis)_thaxter_1892 "chondromyces aurantiacus" (berkeley and curtis) thaxter 1892~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ECT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ECT FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ect]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stigmatella_aurantiaca Stigmatella aurantiaca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ECT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ECT FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=SAM:S~~-~~ADENOSYLMETHIONINE'>SAM</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.42Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>stiE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=41 "~~Chondromyces aurantiacus~~" ~~(Berkeley and Curtis) Thaxter 1892])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ect FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ect OCA], [~~http~~://pdbe.org/6ect PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ect RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ect PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ect ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ect FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ect OCA], [https://pdbe.org/6ect PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ect RCSB], [https://www.ebi.ac.uk/pdbsum/6ect PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ect ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q8RJY2_STIAU Q8RJY2_STIAU]
	Modular type I polyketide synthases (PKSs) produce some of the most chemically complex metabolites in nature through a series of multi-enzyme modules. Each module contains a variety of catalytic domains to selectively tailor the growing molecule. PKS O-methyltransferases ( O-MTs) are predicted to methylate beta-hydroxyl or beta-keto groups but their activity and structure have not been reported. We determined the domain boundaries and characterized the catalytic activity and structure of the StiD and StiE O-MTs, which methylate opposite beta-hydroxyl stereocenters in the myxobacterial stigmatellin biosynthetic pathway. Substrate stereospecificity was demonstrated for the StiD O-MT. Key catalytic residues were identified in the crystal structures and investigated in StiE O-MT via site-directed mutagenesis and further validated with the cyanobacterial CurL O-MT from the curacin biosynthetic pathway. Initial structural and biochemical analysis of PKS O-MTs supplies a new chemoenzymatic tool, with the unique ability to selectively modify hydroxyl groups during polyketide biosynthesis.

	Structural Basis of Polyketide Synthase O-Methylation.,Skiba MA, Bivins MM, Schultz JR, Bernard SM, Fiers WD, Dan Q, Kulkarni S, Wipf P, Gerwick WH, Sherman DH, Aldrich CC, Smith JL ACS Chem Biol. 2018 Nov 29. doi: 10.1021/acschembio.8b00687. PMID:~~30489068<ref>PMID:30489068<~~/~~ref>~~

	~~From MEDLINE®~~/~~PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 6ect" style="background-color:#fffaf0;"><~~/~~div>~~
	~~== References ==~~
	~~<references~~/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bivins, M M~~]]		[[Category: Stigmatella aurantiaca]]
	[[Category: ~~Skiba, M A~~]]		[[Category: Bivins MM]]
	[[Category: ~~Smith, J L~~]]		[[Category: Skiba MA]]
	[[Category: ~~Methyltransferase]]~~		[[Category: Smith JL]]
	~~[[Category: Transferase~~]]

OCA at 09:09, 1 May 2019

2019-05-01T09:09:28Z

← Older revision		Revision as of 09:09, 1 May 2019
Line 1:		Line 1:

	==StiE O-MT residues 961-1257==		==StiE O-MT residues 961-1257==
	<StructureSection load='6ect' size='340' side='right' caption='[[6ect]], [[Resolution\|resolution]] 1.42Å' scene=''>		<StructureSection load='6ect' size='340' side='right'caption='[[6ect]], [[Resolution\|resolution]] 1.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ect]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ECT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ECT FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ect]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"chondromyces_aurantiacus"_(berkeley_and_curtis)_thaxter_1892 "chondromyces aurantiacus" (berkeley and curtis) thaxter 1892]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ECT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ECT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">stiE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=41 "Chondromyces aurantiacus" (Berkeley and Curtis) Thaxter 1892])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ect FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ect OCA], [http://pdbe.org/6ect PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ect RCSB], [http://www.ebi.ac.uk/pdbsum/6ect PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ect ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ect FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ect OCA], [http://pdbe.org/6ect PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ect RCSB], [http://www.ebi.ac.uk/pdbsum/6ect PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ect ProSAT]</span></td></tr>
	</table>		</table>
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Large Structures]]
	[[Category: Bivins, M M]]		[[Category: Bivins, M M]]
	[[Category: Skiba, M A]]		[[Category: Skiba, M A]]

OCA at 06:45, 12 December 2018

2018-12-12T06:45:40Z

@@ Line 1: / Line 1: @@
-The entry 6ect is ON HOLD  until Paper Publication
+==StiE O-MT residues 961-1257==
-Authors:
+Structural Basis of Polyketide Synthase O-Methylation.,Skiba MA, Bivins MM, Schultz JR, Bernard SM, Fiers WD, Dan Q, Kulkarni S, Wipf P, Gerwick WH, Sherman DH, Aldrich CC, Smith JL ACS Chem Biol. 2018 Nov 29. doi: 10.1021/acschembio.8b00687. PMID:30489068<ref>PMID:30489068</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>

OCA at 05:44, 29 August 2018

2018-08-29T05:44:05Z

← Older revision		Revision as of 05:44, 29 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6ect is ON HOLD		The entry 6ect is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ect" [edit=sysop:move=sysop]

2018-08-15T14:54:03Z

Protected "6ect" [edit=sysop:move=sysop]

← Older revision	Revision as of 14:54, 15 August 2018
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6ect is ON HOLD Authors: Description: Category: Unreleased Structures

2018-08-15T14:54:02Z

New page: '''Unreleased structure''' The entry 6ect is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ect is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]