6htm - Revision history

OCA at 11:39, 24 January 2024

2024-01-24T11:39:20Z

← Older revision		Revision as of 11:39, 24 January 2024
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	<StructureSection load='6htm' size='340' side='right'caption='[[6htm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6htm' size='340' side='right'caption='[[6htm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6htm]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"streptomyces_actuosus"_pinnert_et_al. "streptomyces~~ actuosus~~" pinnert et al.~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6HTM FirstGlance]. <br>		<table><tr><td colspan='2'>[[6htm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_actuosus Streptomyces actuosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HTM FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TSS:2-(1H-INDOL-3-YL)ETHANAMINE'>TSS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6htk\|6htk]]</td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TSS:2-(1H-INDOL-3-YL)ETHANAMINE'>TSS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nosL, nocL, DMT42_23170 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1885 "Streptomyces actuosus" Pinnert et al.])</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6htm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htm OCA], [https://pdbe.org/6htm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6htm RCSB], [https://www.ebi.ac.uk/pdbsum/6htm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6htm ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6htm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htm OCA], [~~http~~://pdbe.org/6htm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6htm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6htm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6htm ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/C6FX51_STRAS C6FX51_STRAS]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Streptomyces actuosus pinnert et al]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Amara, P]]		[[Category: Streptomyces actuosus]]
	[[Category: Bella, M]]		[[Category: Amara P]]
	[[Category: Gambarelli, S]]		[[Category: Bella M]]
	[[Category: Mouesca~~, J M~~]]		[[Category: Gambarelli S]]
	[[Category: Nicolet, Y]]		[[Category: Mouesca JM]]
	[[Category: Saragaglia, C]]		[[Category: Nicolet Y]]
	~~[[Category: Antibiotic]]~~		[[Category: Saragaglia C]]
	~~[[Category: Nosiheptide]]~~
	~~[[Category: Radical sam]]~~
	~~[[Category: Tryptophan lyase~~]]

OCA at 07:59, 24 April 2019

2019-04-24T07:59:22Z

← Older revision		Revision as of 07:59, 24 April 2019
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	==X-ray structure of the tryptophan lyase NosL in complex with bound tryptamin==		==X-ray structure of the tryptophan lyase NosL in complex with bound tryptamin==
	<StructureSection load='6htm' size='340' side='right' caption='[[6htm]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6htm' size='340' side='right'caption='[[6htm]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6htm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTM FirstGlance]. <br>		<table><tr><td colspan='2'>[[6htm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTM FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Streptomyces actuosus pinnert et al]]		[[Category: Streptomyces actuosus pinnert et al]]
			[[Category: Large Structures]]
	[[Category: Amara, P]]		[[Category: Amara, P]]
	[[Category: Bella, M]]		[[Category: Bella, M]]

OCA at 21:07, 2 December 2018

2018-12-02T21:07:22Z

← Older revision		Revision as of 21:07, 2 December 2018
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6htm is ~~ON HOLD until Paper~~ Publication		==X-ray structure of the tryptophan lyase NosL in complex with bound tryptamin==
			<StructureSection load='6htm' size='340' side='right' caption='[[6htm]], [[Resolution\|resolution]] 1.70Å' scene=''>
			== Structural highlights ==
			<table><tr><td colspan='2'>[[6htm]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_actuosus"_pinnert_et_al. "streptomyces actuosus" pinnert et al.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTM FirstGlance]. <br>
			</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5AD:5-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=TSS:2-(1H-INDOL-3-YL)ETHANAMINE'>TSS</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6htk\|6htk]]</td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nosL, nocL, DMT42_23170 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1885 "Streptomyces actuosus" Pinnert et al.])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6htm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htm OCA], [http://pdbe.org/6htm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6htm RCSB], [http://www.ebi.ac.uk/pdbsum/6htm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6htm ProSAT]</span></td></tr>
			</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The radical S-adenosyl-l-methionine tryptophan lyase uses radical-based chemistry to convert l-tryptophan into 3-methyl-2-indolic acid, a fragment in the biosynthesis of the thiopeptide antibiotic nosiheptide. This complex reaction involves several successive steps corresponding to (i) the activation by a specific hydrogen-atom abstraction, (ii) an unprecedented CO2(-) radical migration, (iii) a cyanide fragment release, and (iv) the termination of the radical-based reaction. In vitro study of this reaction is made more difficult because the enzyme produces a significant amount of a shunt product instead of the natural product. Here, using a combination of X-ray crystallography, electron paramagnetic resonance spectroscopy, and quantum and hybrid quantum mechanical/molecular mechanical calculations, we have deciphered the fine mechanism of the key CO2(-) radical migration, highlighting how the preorganized active site of the protein tightly controls this reaction.

	~~Authors~~: Amara, P., Mouesca, J.M~~., Bella~~, M., Saragaglia, C., Gambarelli, S., Nicolet, Y.		Radical S-Adenosyl-l-methionine Tryptophan Lyase (NosL): How the Protein Controls the Carboxyl Radical *CO2(-) Migration.,Amara P, Mouesca JM, Bella M, Martin L, Saragaglia C, Gambarelli S, Nicolet Y J Am Chem Soc. 2018 Nov 16. doi: 10.1021/jacs.8b09142. PMID:30418774<ref>PMID:30418774</ref>

	~~Description: X-ray structure~~ of the ~~tryptophan lyase NosL in complex with bound tryptamin~~		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	[[Category: ~~Unreleased Structures~~]]		</div>
			<div class="pdbe-citations 6htm" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
			__TOC__
			</StructureSection>
			[[Category: Streptomyces actuosus pinnert et al]]
			[[Category: Amara, P]]
	[[Category: Bella, M]]		[[Category: Bella, M]]
	[[Category: Gambarelli, S]]		[[Category: Gambarelli, S]]
			[[Category: Mouesca, J M]]
	[[Category: Nicolet, Y]]		[[Category: Nicolet, Y]]
	~~[[Category: Amara, P]]~~
	[[Category: Saragaglia, C]]		[[Category: Saragaglia, C]]
	[[Category: ~~Mouesca, J.M~~]]		[[Category: Antibiotic]]
			[[Category: Nosiheptide]]
			[[Category: Radical sam]]
			[[Category: Tryptophan lyase]]

OCA at 06:13, 17 October 2018

2018-10-17T06:13:26Z

← Older revision		Revision as of 06:13, 17 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6htm is ON HOLD		The entry 6htm is ON HOLD until Paper Publication

	Authors: Amara, P., Mouesca, J.M., Bella, M., Saragaglia, C., Gambarelli, S., Nicolet, Y.		Authors: Amara, P., Mouesca, J.M., Bella, M., Saragaglia, C., Gambarelli, S., Nicolet, Y.

OCA: Protected "6htm" [edit=sysop:move=sysop]

2018-10-10T06:16:47Z

Protected "6htm" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:16, 10 October 2018
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6htm is ON HOLD Authors: Amara, P., Mouesca, J.M., Bella, M., Saragaglia, C., Gambarelli, S., Nicolet, Y. Description: X-ray structure of the trypt...

2018-10-10T06:16:45Z

New page: '''Unreleased structure''' The entry 6htm is ON HOLD Authors: Amara, P., Mouesca, J.M., Bella, M., Saragaglia, C., Gambarelli, S., Nicolet, Y. Description: X-ray structure of the trypt...

New page

'''Unreleased structure'''

The entry 6htm is ON HOLD

Authors: Amara, P., Mouesca, J.M., Bella, M., Saragaglia, C., Gambarelli, S., Nicolet, Y.

Description: X-ray structure of the tryptophan lyase NosL in complex with bound tryptamin
[[Category: Unreleased Structures]]
[[Category: Bella, M]]
[[Category: Gambarelli, S]]
[[Category: Nicolet, Y]]
[[Category: Amara, P]]
[[Category: Saragaglia, C]]
[[Category: Mouesca, J.M]]