6k4e - Revision history

OCA at 19:27, 29 May 2024

2024-05-29T19:27:16Z

← Older revision		Revision as of 19:27, 29 May 2024
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	<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K4E FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.094Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>NCTC11839_02430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [https://pdbe.org/6k4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [https://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [https://pdbe.org/6k4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [https://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/Q9I6W0_PSEAE Q9I6W0_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lescar, J~~]]		[[Category: Pseudomonas aeruginosa]]
	[[Category: ~~Lin,~~ J Q]]		[[Category: Lescar J]]
	[[Category: ~~Biofilm]]~~		[[Category: Lin JQ]]
	~~[[Category: Pp2c]]~~
	~~[[Category: Signaling protein~~]]

OCA at 15:53, 3 November 2021

2021-11-03T15:53:14Z

← Older revision		Revision as of 15:53, 3 November 2021
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	<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6K4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6K4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCTC11839_02430 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCTC11839_02430 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [~~http~~://pdbe.org/6k4e PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [https://pdbe.org/6k4e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [https://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Jianqing~~, L]]		[[Category: Lescar, J]]
	[[Category: ~~Julien~~, L]]		[[Category: Lin, J Q]]
	[[Category: Biofilm]]		[[Category: Biofilm]]
	[[Category: Pp2c]]		[[Category: Pp2c]]
	[[Category: Signaling protein]]		[[Category: Signaling protein]]

OCA at 07:05, 25 November 2020

2020-11-25T07:05:07Z

← Older revision		Revision as of 07:05, 25 November 2020
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	<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6K4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6K4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCTC11839_02430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCTC11839_02430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [http://pdbe.org/6k4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [http://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [http://pdbe.org/6k4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [http://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The critical role of bacterial biofilms in chronic human infections calls for novel anti-biofilm strategies targeting the regulation of biofilm development. However, the regulation of biofilm development is very complex and can include multiple, highly interconnected signal transduction/response pathways, which are incompletely understood. We demonstrated previously that in the opportunistic, human pathogen P. aeruginosa, the PP2C-like protein phosphatase SiaA and the di-guanylate cyclase SiaD control the formation of macroscopic cellular aggregates, a type of suspended biofilms, in response to surfactant stress. In this study, we demonstrate that the SiaABC proteins represent a signal response pathway that functions through a partner switch mechanism to control biofilm formation. We also demonstrate that SiaABCD functionality is dependent on carbon substrate availability for a variety of substrates, and that upon carbon starvation, SiaB mutants show impaired dispersal, in particular with the primary fermentation product ethanol. This suggests that carbon availability is at least one of the key environmental cues integrated by the SiaABCD system. Further, our biochemical, physiological and crystallographic data reveals that the phosphatase SiaA and its kinase counterpart SiaB balance the phosphorylation status of their target protein SiaC at threonine 68 (T68). Crystallographic analysis of the SiaA-PP2C domain shows that SiaA is present as a dimer. Dynamic modelling of SiaA with SiaC suggested that SiaA interacts strongly with phosphorylated SiaC and dissociates rapidly upon dephosphorylation of SiaC. Further, we show that the known phosphatase inhibitor fumonisin inhibits SiaA mediated phosphatase activity in vitro. In conclusion, the present work improves our understanding of how P. aeuruginosa integrates specific environmental conditions, such as carbon availability and surfactant stress, to regulate cellular aggregation and biofilm formation. With the biochemical and structural characterization of SiaA, initial data on the catalytic inhibition of SiaA, and the interaction between SiaA and SiaC, our study identifies promising targets for the development of biofilm-interference drugs to combat infections of this aggressive opportunistic pathogen.

			The SiaABC threonine phosphorylation pathway controls biofilm formation in response to carbon availability in Pseudomonas aeruginosa.,Poh WH, Lin J, Colley B, Muller N, Goh BC, Schleheck D, El Sahili A, Marquardt A, Liang Y, Kjelleberg S, Lescar J, Rice SA, Klebensberger J PLoS One. 2020 Nov 6;15(11):e0241019. doi: 10.1371/journal.pone.0241019., eCollection 2020. PMID:33156827<ref>PMID:33156827</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6k4e" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:08, 2 October 2019

2019-10-02T11:08:40Z

← Older revision		Revision as of 11:08, 2 October 2019
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	<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>		<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K4E FirstGlance]. <br>		<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K4E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">NCTC11839_02430 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [http://pdbe.org/6k4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [http://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [http://pdbe.org/6k4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [http://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>
	</table>		</table>

OCA at 06:54, 26 June 2019

2019-06-26T06:54:36Z

← Older revision		Revision as of 06:54, 26 June 2019
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 6k4e is ~~ON HOLD until Paper Publication~~		==SiaA-PP2C domain of Pseudomonas aeruginosa==
			<StructureSection load='6k4e' size='340' side='right'caption='[[6k4e]], [[Resolution\|resolution]] 2.09Å' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>[[6k4e]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6K4E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6K4E FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	[[Category: ~~Unreleased Structures~~]]		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6k4e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6k4e OCA], [http://pdbe.org/6k4e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6k4e RCSB], [http://www.ebi.ac.uk/pdbsum/6k4e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6k4e ProSAT]</span></td></tr>
			</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Jianqing, L]]
			[[Category: Julien, L]]
			[[Category: Biofilm]]
			[[Category: Pp2c]]
			[[Category: Signaling protein]]

OCA at 20:58, 5 June 2019

2019-06-05T20:58:40Z

← Older revision		Revision as of 20:58, 5 June 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6k4e is ON HOLD		The entry 6k4e is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6k4e" [edit=sysop:move=sysop]

2019-05-29T04:27:35Z

Protected "6k4e" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:27, 29 May 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6k4e is ON HOLD Authors: Description: Category: Unreleased Structures

2019-05-29T04:27:33Z

New page: '''Unreleased structure''' The entry 6k4e is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6k4e is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]