6o7a - Revision history

OCA at 14:50, 13 March 2024

2024-03-13T14:50:55Z

← Older revision		Revision as of 14:50, 13 March 2024
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	<StructureSection load='6o7a' size='340' side='right'caption='[[6o7a]], [[Resolution\|resolution]] 3.30Å' scene=''>		<StructureSection load='6o7a' size='340' side='right'caption='[[6o7a]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6o7a]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lotja Lotja~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O7A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6O7A FirstGlance]. <br>		<table><tr><td colspan='2'>[[6o7a]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Lotus_japonicus Lotus japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6O7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6O7A FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~CASTOR (~~[~~http://www.ncbi.nlm.nih~~.~~gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5~~&~~id=34305 LOTJA])~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.3Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6o7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o7a OCA], [~~http~~://pdbe.org/6o7a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6o7a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6o7a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6o7a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6o7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6o7a OCA], [https://pdbe.org/6o7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6o7a RCSB], [https://www.ebi.ac.uk/pdbsum/6o7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6o7a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/CASTO_LOTJA CASTO_LOTJA]] Ion channel with a moderate preference for potassium over sodium and calcium. Involved in perinuclear calcium spiking but not in cytosolic calcium influx. Closed at negative voltages in presence of magnesium. Required for early signal transduction events leading to endosymbiosis. Acts early in a signal transduction chain leading from the perception of Nod factor to the activation of calcium spiking. Also involved in fungal entry into root epidermal cells during the establishment of the arbuscular mycorrhizal symbiosis.<ref>PMID:16903357</ref> <ref>PMID:19106374</ref>		[https://www.uniprot.org/uniprot/CASTO_LOTJA CASTO_LOTJA] Ion channel with a moderate preference for potassium over sodium and calcium. Involved in perinuclear calcium spiking but not in cytosolic calcium influx. Closed at negative voltages in presence of magnesium. Required for early signal transduction events leading to endosymbiosis. Acts early in a signal transduction chain leading from the perception of Nod factor to the activation of calcium spiking. Also involved in fungal entry into root epidermal cells during the establishment of the arbuscular mycorrhizal symbiosis.<ref>PMID:16903357</ref> <ref>PMID:19106374</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	A family of plant nuclear ion channels, including DMI1 (Does not Make Infections 1) and its homologs CASTOR and POLLUX, are required for the establishment of legume-microbe symbioses by generating nuclear and perinuclear Ca(2+) spiking. Here we show that CASTOR from Lotus japonicus is a highly selective Ca(2+) channel whose activation requires cytosolic/nucleosolic Ca(2+), contrary to the previous suggestion of it being a K(+) channel. Structurally, the cytosolic/nucleosolic ligand-binding soluble region of CASTOR contains two tandem RCK (Regulator of Conductance for K(+)) domains, and four subunits assemble into the gating ring architecture, similar to that of large conductance, Ca(2+)-gated K(+) (BK) channels despite the lack of sequence similarity. Multiple ion binding sites are clustered at two locations within each subunit, and three of them are identified to be Ca(2+) sites. Our in vitro and in vivo assays also demonstrate the importance of these gating-ring Ca(2+) binding sites to the physiological function of CASTOR as well as DMI1.

	Ca(2+)-regulated Ca(2+) channels with an RCK gating ring control plant symbiotic associations.,Kim S, Zeng W, Bernard S, Liao J, Venkateshwaran M, Ane JM, Jiang Y Nat Commun. 2019 Aug 16;10(1):3703. doi: 10.1038/s41467-019-11698-5. PMID:31420535<ref>PMID:31420535</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 6o7a" style="background-color:#fffaf0;"></div~~>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Lotja~~]]		[[Category: Lotus japonicus]]
	[[Category: Jiang, Y]]		[[Category: Jiang Y]]
	[[Category: Kim, S]]		[[Category: Kim S]]
	~~[[Category: Calcium channel]]~~
	~~[[Category: Castor]]~~
	~~[[Category: Gating ring]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Rck domain]]~~
	~~[[Category: Transport protein~~]]

OCA at 17:41, 20 November 2019

2019-11-20T17:41:11Z

@@ Line 1: / Line 1: @@
-The entry 6o7a is ON HOLD  until Paper Publication
+==Crystal structure of the LjCASTOR gating ring in the Ca2+-free state==
-Authors: Jiang, Y., Kim, S.
+Ca(2+)-regulated Ca(2+) channels with an RCK gating ring control plant symbiotic associations.,Kim S, Zeng W, Bernard S, Liao J, Venkateshwaran M, Ane JM, Jiang Y Nat Commun. 2019 Aug 16;10(1):3703. doi: 10.1038/s41467-019-11698-5. PMID:31420535<ref>PMID:31420535</ref>
-Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
 [[Category: Kim, S]]
-[[Category: Jiang, Y]]
+[[Category: Calcium channel]]

OCA at 05:57, 20 March 2019

2019-03-20T05:57:14Z

← Older revision		Revision as of 05:57, 20 March 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6o7a is ON HOLD		The entry 6o7a is ON HOLD until Paper Publication

	Authors: Jiang, Y., Kim, S.		Authors: Jiang, Y., Kim, S.
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	Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state		Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Kim, S]]
	[[Category: Jiang, Y]]		[[Category: Jiang, Y]]
	~~[[Category: Kim, S]]~~

OCA: Protected "6o7a" [edit=sysop:move=sysop]

2019-03-13T10:19:06Z

Protected "6o7a" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:19, 13 March 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6o7a is ON HOLD Authors: Jiang, Y., Kim, S. Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state [[Category: Unrelease...

2019-03-13T10:19:04Z

New page: '''Unreleased structure''' The entry 6o7a is ON HOLD Authors: Jiang, Y., Kim, S. Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 6o7a is ON HOLD

Authors: Jiang, Y., Kim, S.

Description: Crystal structure of the LjCASTOR gating ring in the Ca2+-free state
[[Category: Unreleased Structures]]
[[Category: Jiang, Y]]
[[Category: Kim, S]]