6ocr - Revision history

OCA at 14:51, 13 March 2024

2024-03-13T14:51:30Z

← Older revision		Revision as of 14:51, 13 March 2024
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	<StructureSection load='6ocr' size='340' side='right'caption='[[6ocr]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='6ocr' size='340' side='right'caption='[[6ocr]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ocr]] is a 15 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OCR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OCR FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ocr]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OCR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OCR FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~KCTD16~~, ~~KIAA1317 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5~~&~~id=9606 HUMAN])~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.28Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ocr OCA], [~~http~~://pdbe.org/6ocr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6ocr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6ocr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6ocr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ocr OCA], [https://pdbe.org/6ocr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ocr RCSB], [https://www.ebi.ac.uk/pdbsum/6ocr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ocr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/KCD16_HUMAN KCD16_HUMAN]] Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).		[https://www.uniprot.org/uniprot/KCD16_HUMAN KCD16_HUMAN] Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Metabotropic GABAB receptors mediate a significant fraction of inhibitory neurotransmission in the brain. Native GABAB receptor complexes contain the principal subunits GABAB1 and GABAB2, which form an obligate heterodimer, and auxiliary subunits, known as potassium channel tetramerization domain-containing proteins (KCTDs). KCTDs interact with GABAB receptors and modify the kinetics of GABAB receptor signaling. Little is known about the molecular mechanism governing the direct association and functional coupling of GABAB receptors with these auxiliary proteins. Here, we describe the high-resolution structure of the KCTD16 oligomerization domain in complex with part of the GABAB2 receptor. A single GABAB2 C-terminal peptide is bound to the interior of an open pentamer formed by the oligomerization domain of five KCTD16 subunits. Mutation of specific amino acids identified in the structure of the GABAB2-KCTD16 interface disrupted both the biochemical association and functional modulation of GABAB receptors and G protein-activated inwardly rectifying K(+) channel (GIRK) channels. These interfacial residues are conserved among KCTDs, suggesting a common mode of KCTD interaction with GABAB receptors. Defining the binding interface of GABAB receptor and KCTD reveals a potential regulatory site for modulating GABAB-receptor function in the brain.

	Structural basis for auxiliary subunit KCTD16 regulation of the GABAB receptor.,Zuo H, Glaaser I, Zhao Y, Kurinov I, Mosyak L, Wang H, Liu J, Park J, Frangaj A, Sturchler E, Zhou M, McDonald P, Geng Y, Slesinger PA, Fan QR Proc Natl Acad Sci U S A. 2019 Apr 10. pii: 1903024116. doi:, 10.1073/pnas.1903024116. PMID:30971491<ref>PMID:30971491</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 6ocr" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fan~~, Q R~~]]		[[Category: Fan QR]]
	[[Category: Frangaj, A]]		[[Category: Frangaj A]]
	[[Category: Geng, Y]]		[[Category: Geng Y]]
	[[Category: Glaaser, I]]		[[Category: Glaaser I]]
	[[Category: Kurinov, I]]		[[Category: Kurinov I]]
	[[Category: Liu, J]]		[[Category: Liu J]]
	[[Category: McDonald, P]]		[[Category: McDonald P]]
	[[Category: Mosyak, L]]		[[Category: Mosyak L]]
	[[Category: Park, J]]		[[Category: Park J]]
	[[Category: Slesinger~~, P A~~]]		[[Category: Slesinger PA]]
	[[Category: Sturchler, E]]		[[Category: Sturchler E]]
	[[Category: Wang, H]]		[[Category: Wang H]]
	[[Category: Zhao, Y]]		[[Category: Zhao Y]]
	[[Category: Zhou, M]]		[[Category: Zhou M]]
	[[Category: Zuo, H]]		[[Category: Zuo H]]
	~~[[Category: Kctd16]]~~
	~~[[Category: Pentamer]]~~
	~~[[Category: Signaling protein~~]]

OCA at 08:21, 24 April 2019

2019-04-24T08:21:25Z

← Older revision		Revision as of 08:21, 24 April 2019
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	<StructureSection load='6ocr' size='340' side='right'caption='[[6ocr]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='6ocr' size='340' side='right'caption='[[6ocr]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ocr]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OCR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OCR FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ocr]] is a 15 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OCR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OCR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ocr OCA], [http://pdbe.org/6ocr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ocr RCSB], [http://www.ebi.ac.uk/pdbsum/6ocr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ocr ProSAT]</span></td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KCTD16, KIAA1317 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ocr OCA], [http://pdbe.org/6ocr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ocr RCSB], [http://www.ebi.ac.uk/pdbsum/6ocr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ocr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/KCD16_HUMAN KCD16_HUMAN]] Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).		[[http://www.uniprot.org/uniprot/KCD16_HUMAN KCD16_HUMAN]] Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Metabotropic GABAB receptors mediate a significant fraction of inhibitory neurotransmission in the brain. Native GABAB receptor complexes contain the principal subunits GABAB1 and GABAB2, which form an obligate heterodimer, and auxiliary subunits, known as potassium channel tetramerization domain-containing proteins (KCTDs). KCTDs interact with GABAB receptors and modify the kinetics of GABAB receptor signaling. Little is known about the molecular mechanism governing the direct association and functional coupling of GABAB receptors with these auxiliary proteins. Here, we describe the high-resolution structure of the KCTD16 oligomerization domain in complex with part of the GABAB2 receptor. A single GABAB2 C-terminal peptide is bound to the interior of an open pentamer formed by the oligomerization domain of five KCTD16 subunits. Mutation of specific amino acids identified in the structure of the GABAB2-KCTD16 interface disrupted both the biochemical association and functional modulation of GABAB receptors and G protein-activated inwardly rectifying K(+) channel (GIRK) channels. These interfacial residues are conserved among KCTDs, suggesting a common mode of KCTD interaction with GABAB receptors. Defining the binding interface of GABAB receptor and KCTD reveals a potential regulatory site for modulating GABAB-receptor function in the brain.

			Structural basis for auxiliary subunit KCTD16 regulation of the GABAB receptor.,Zuo H, Glaaser I, Zhao Y, Kurinov I, Mosyak L, Wang H, Liu J, Park J, Frangaj A, Sturchler E, Zhou M, McDonald P, Geng Y, Slesinger PA, Fan QR Proc Natl Acad Sci U S A. 2019 Apr 10. pii: 1903024116. doi:, 10.1073/pnas.1903024116. PMID:30971491<ref>PMID:30971491</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6ocr" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fan, Q R]]		[[Category: Fan, Q R]]

OCA at 07:29, 10 April 2019

2019-04-10T07:29:26Z

← Older revision		Revision as of 07:29, 10 April 2019
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 6ocr ~~is ON HOLD~~		==Crystal structure of human KCTD16 T1 domain==
			<StructureSection load='6ocr' size='340' side='right'caption='[[6ocr]], [[Resolution\|resolution]] 2.28Å' scene=''>
	~~Authors~~: ~~Zuo, H~~.~~, Glaaser, I~~.~~, Zhao, Y~~.~~, Kurinov, I~~.~~, Mosyak, L~~., ~~Wang, H~~.~~, Liu, J~~.~~, Park, J~~., ~~Frangaj, A~~., ~~Sturchler, E~~.~~, Zhou, M~~.~~, McDonald, P~~., ~~Geng, Y~~.~~, Slesinger, P~~.A., ~~Fan, Q~~.R.		== Structural highlights ==
			<table><tr><td colspan='2'>[[6ocr]] is a 15 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OCR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6OCR FirstGlance]. <br>
	~~Description~~: ~~Crystal structure~~ of ~~human KCTD16 T1 domain~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ocr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ocr OCA], [http://pdbe.org/6ocr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ocr RCSB], [http://www.ebi.ac.uk/pdbsum/6ocr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ocr ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
			== Function ==
			[[http://www.uniprot.org/uniprot/KCD16_HUMAN KCD16_HUMAN]] Auxiliary subunit of GABA-B receptors that determine the pharmacology and kinetics of the receptor response. Increases agonist potency and markedly alter the G-protein signaling of the receptors by accelerating onset and promoting desensitization (By similarity).
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Fan, Q R]]
	[[Category: Frangaj, A]]		[[Category: Frangaj, A]]
	[[Category: ~~Mcdonald~~, P]]		[[Category: Geng, Y]]
	[[Category: ~~Fan~~, ~~Q.R~~]]		[[Category: Glaaser, I]]
			[[Category: Kurinov, I]]
			[[Category: Liu, J]]
			[[Category: McDonald, P]]
			[[Category: Mosyak, L]]
	[[Category: Park, J]]		[[Category: Park, J]]
	[[Category: ~~Mosyak~~, L]]		[[Category: Slesinger, P A]]
	[[Category: ~~Liu~~, J]]		[[Category: Sturchler, E]]
	[[Category: Wang, H]]		[[Category: Wang, H]]
			[[Category: Zhao, Y]]
			[[Category: Zhou, M]]
	[[Category: Zuo, H]]		[[Category: Zuo, H]]
	[[Category: ~~Slesinger, P.A~~]]		[[Category: Kctd16]]
	[[Category: ~~Zhou, M~~]]		[[Category: Pentamer]]
	[[Category: ~~Zhao, Y]]~~		[[Category: Signaling protein]]
	~~[[Category: Glaaser, I]]~~
	~~[[Category: Kurinov, I]]~~
	~~[[Category: Geng, Y]]~~
	~~[[Category: Sturchler, E~~]]

OCA: Protected "6ocr" [edit=sysop:move=sysop]

2019-04-03T04:40:09Z

Protected "6ocr" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:40, 3 April 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6ocr is ON HOLD Authors: Zuo, H., Glaaser, I., Zhao, Y., Kurinov, I., Mosyak, L., Wang, H., Liu, J., Park, J., Frangaj, A., Sturchler, E., Zhou, M.,...

2019-04-03T04:40:07Z

New page: '''Unreleased structure''' The entry 6ocr is ON HOLD Authors: Zuo, H., Glaaser, I., Zhao, Y., Kurinov, I., Mosyak, L., Wang, H., Liu, J., Park, J., Frangaj, A., Sturchler, E., Zhou, M.,...

New page

'''Unreleased structure'''

The entry 6ocr is ON HOLD

Authors: Zuo, H., Glaaser, I., Zhao, Y., Kurinov, I., Mosyak, L., Wang, H., Liu, J., Park, J., Frangaj, A., Sturchler, E., Zhou, M., McDonald, P., Geng, Y., Slesinger, P.A., Fan, Q.R.

Description: Crystal structure of human KCTD16 T1 domain
[[Category: Unreleased Structures]]
[[Category: Frangaj, A]]
[[Category: Mcdonald, P]]
[[Category: Fan, Q.R]]
[[Category: Park, J]]
[[Category: Mosyak, L]]
[[Category: Liu, J]]
[[Category: Wang, H]]
[[Category: Zuo, H]]
[[Category: Slesinger, P.A]]
[[Category: Zhou, M]]
[[Category: Zhao, Y]]
[[Category: Glaaser, I]]
[[Category: Kurinov, I]]
[[Category: Geng, Y]]
[[Category: Sturchler, E]]