6oop - Revision history

OCA at 14:52, 13 March 2024

2024-03-13T14:52:41Z

← Older revision		Revision as of 14:52, 13 March 2024
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	<StructureSection load='6oop' size='340' side='right'caption='[[6oop]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='6oop' size='340' side='right'caption='[[6oop]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6oop]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6OOP FirstGlance]. <br>		<table><tr><td colspan='2'>[[6oop]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OOP FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=KHJ:1,1~~-~~dimethyl-4~~,~~4-bipyridin-1-ium'>KHJ</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~A9R57_05385~~, ~~BJJ90_17655 ([http:~~/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KHJ:1,1-dimethyl-4,4-bipyridin-1-ium'>KHJ</scene>, <scene name='pdbligand=PR:PRASEODYMIUM+ION'>PR</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6oop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oop OCA], [~~http~~://pdbe.org/6oop PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6oop RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6oop PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6oop ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oop OCA], [https://pdbe.org/6oop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oop RCSB], [https://www.ebi.ac.uk/pdbsum/6oop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oop ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/MDFA_ECOLI MDFA_ECOLI] Efflux pump driven by the proton motive force. Confers resistance to a broad spectrum of chemically unrelated drugs. Confers resistance to a diverse group of cationic or zwitterionic lipophilic compounds such as ethidium bromide, tetraphenylphosphonium, rhodamine, daunomycin, benzalkonium, rifampicin, tetracycline, puromycin, and to chemically unrelated, clinically important antibiotics such as chloramphenicol, erythromycin, and certain aminoglycosides and fluoroquinolones. Overexpression results in isopropyl-beta-D-thiogalactopyranoside (IPTG) exclusion and spectinomycin sensitivity. Transport of neutral substrates is electrogenic, whereas transport of cationic substrates is electroneutral. In addition to its role in multidrug resistance, confers extreme alkaline pH resistance, allowing the growth under conditions that are close to those used normally by alkaliphiles. This activity requires Na(+) or K(+).<ref>PMID:12578981</ref> <ref>PMID:15371593</ref> <ref>PMID:9079913</ref> <ref>PMID:9644262</ref> <ref>PMID:9811673</ref>
	~~MdfA is~~ a ~~prototypical H(+)-coupled multidrug transporter that is characterized by extraordinarily~~ broad ~~substrate specificity~~. ~~The involvement~~ of ~~specific H-bonds in MdfA-drug interactions~~ and ~~the simplicity of altering the substrate specificity of MdfA contradict the promiscuous nature of multidrug recognition~~, ~~presenting a baffling conundrum~~. ~~Here we show the X-ray structures of MdfA variant I239T/G354E~~ in complexes with three electrically different ligands, determined at resolutions up to 2.2 A. Our structures reveal that I239T/G354E interacts with these compounds differently from MdfA and that I239T/G354E possesses two discrete, non-~~overlapping substrate~~-~~binding sites. Our results shed new light on the molecular design of multidrug~~-~~binding~~ and ~~protonation sites and highlight the importance~~ of ~~often-neglected~~, ~~long-range charge-charge interactions in multidrug recognition~~. ~~Beyond helping~~ to ~~solve the ostensible conundrum of~~ multidrug ~~recognition~~, ~~our findings suggest~~ the ~~mechanistic difference between substrate and inhibitor for any H~~(+)~~-dependent multidrug transporter, which may open new vistas on curtailing efflux-mediated multidrug resistance~~.

	~~Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition.,Wu HH, Symersky J, Lu M Commun Biol. 2019 Jun 17;2~~:~~210. doi: 10.1038~~/~~s42003-019-0446-y. eCollection 2019.~~ PMID:~~31240248~~<ref>PMID:~~31240248~~</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>
	</~~div~~>
	<~~div class="pdbe-citations 6oop" style="background-color~~:~~#fffaf0;">~~</~~div~~>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lu, M]]		[[Category: Lu M]]
	~~[[Category: Complex]]~~
	~~[[Category: Transport]]~~
	~~[[Category: Transport protein~~]]

OCA at 11:54, 1 January 2020

2020-01-01T11:54:46Z

@@ Line 1: / Line 1: @@
-The entry 6oop is ON HOLD  until Paper Publication
+==protein B==
-Authors: Lu, M.
+Structure of an engineered multidrug transporter MdfA reveals the molecular basis for substrate recognition.,Wu HH, Symersky J, Lu M Commun Biol. 2019 Jun 17;2:210. doi: 10.1038/s42003-019-0446-y. eCollection 2019. PMID:31240248<ref>PMID:31240248</ref>
-Description: protein B
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
 [[Category: Lu, M]]

OCA at 03:58, 12 June 2019

2019-06-12T03:58:15Z

← Older revision		Revision as of 03:58, 12 June 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6oop is ON HOLD		The entry 6oop is ON HOLD until Paper Publication

	Authors: Lu, M.		Authors: Lu, M.

OCA: Protected "6oop" [edit=sysop:move=sysop]

2019-05-01T07:14:31Z

Protected "6oop" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:14, 1 May 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6oop is ON HOLD Authors: Lu, M. Description: protein B Category: Unreleased Structures Category: Lu, M

2019-05-01T07:14:30Z

New page: '''Unreleased structure''' The entry 6oop is ON HOLD Authors: Lu, M. Description: protein B Category: Unreleased Structures Category: Lu, M

New page

'''Unreleased structure'''

The entry 6oop is ON HOLD

Authors: Lu, M.

Description: protein B
[[Category: Unreleased Structures]]
[[Category: Lu, M]]