6qo7 - Revision history

OCA at 16:07, 28 August 2019

2019-08-28T16:07:45Z

← Older revision		Revision as of 16:07, 28 August 2019
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	<StructureSection load='6qo7' size='340' side='right'caption='[[6qo7]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='6qo7' size='340' side='right'caption='[[6qo7]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6qo7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QO7 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6qo7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_(strain_sterne) Bacillus anthracis (strain sterne)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QO7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">nrdF, GBAA_1372 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=260799 Bacillus anthracis (strain Sterne)])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qo7 OCA], [http://pdbe.org/6qo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qo7 RCSB], [http://www.ebi.ac.uk/pdbsum/6qo7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qo7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qo7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qo7 OCA], [http://pdbe.org/6qo7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qo7 RCSB], [http://www.ebi.ac.uk/pdbsum/6qo7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qo7 ProSAT]</span></td></tr>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/Q81TB4_BACAN Q81TB4_BACAN]] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.[PIRNR:PIRNR000355]		[[http://www.uniprot.org/uniprot/Q81TB4_BACAN Q81TB4_BACAN]] Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.[PIRNR:PIRNR000355]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Class Ib ribonucleotide reductases (RNR) utilize a di-nuclear manganese or iron cofactor for reduction of superoxide or molecular oxygen, respectively. This generates a stable tyrosyl radical (Y.) in the R2 subunit (NrdF), which is further used for ribonucleotide reduction in the R1 subunit of RNR. Here, we report high-resolution crystal structures of Bacillus anthracis NrdF in the metal-free form (1.51 A) and in complex with manganese (Mn(II)/Mn(II), 1.30 A). We also report three structures of the protein in complex with iron, either prepared anaerobically (Fe(II)/Fe(II) form, 1.32 A), or prepared aerobically in the photo-reduced Fe(II)/Fe(II) form (1.63 A) and with the partially oxidized metallo-cofactor (1.46 A). The structures reveal significant conformational dynamics, likely to be associated with the generation, stabilization, and transfer of the radical to the R1 subunit. Based on observed redox-dependent structural changes, we propose that the passage for the superoxide, linking the FMN cofactor of NrdI and the metal site in NrdF, is closed upon metal oxidation, blocking access to the metal and radical sites. In addition, we describe the structural mechanics likely to be involved in this process.

			Redox-induced structural changes in the di-iron and di-manganese forms of Bacillus anthracis ribonucleotide reductase subunit NrdF suggest a mechanism for gating of radical access.,Grave K, Lambert W, Berggren G, Griese JJ, Bennett MD, Logan DT, Hogbom M J Biol Inorg Chem. 2019 Aug 13. pii: 10.1007/s00775-019-01703-z. doi:, 10.1007/s00775-019-01703-z. PMID:31410573<ref>PMID:31410573</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6qo7" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:09, 21 August 2019

2019-08-21T06:09:27Z

@@ Line 1: / Line 1: @@
-The entry 6qo7 is ON HOLD  until Paper Publication
+==Crystal structure of ribonucleotide reductase NrdF from Bacillus anthracis aerobically soaked with ferrous ions (photo-reduced)==
+<StructureSection load='6qo7' size='340' side='right'caption='[[6qo7]], [[Resolution|resolution]] 1.63&Aring;' scene=''>
-Authors:
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6qo7]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QO7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QO7 FirstGlance]. <br>
-Description:
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
-[[Category: Unreleased Structures]]
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonucleoside-diphosphate_reductase Ribonucleoside-diphosphate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.17.4.1 1.17.4.1] </span></td></tr>

OCA at 13:53, 27 February 2019

2019-02-27T13:53:39Z

← Older revision		Revision as of 13:53, 27 February 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6qo7 is ON HOLD		The entry 6qo7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6qo7" [edit=sysop:move=sysop]

2019-02-21T05:23:49Z

Protected "6qo7" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:23, 21 February 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6qo7 is ON HOLD Authors: Description: Category: Unreleased Structures

2019-02-21T05:23:48Z

New page: '''Unreleased structure''' The entry 6qo7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6qo7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]