6tch - Revision history

OCA at 10:42, 15 November 2023

2023-11-15T10:42:10Z

← Older revision		Revision as of 10:42, 15 November 2023
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	==Binary complex of 14-3-3 sigma and a high-affinity non-canonical 9-mer peptide binder==		==Binary complex of 14-3-3 sigma and a high-affinity non-canonical 9-mer peptide binder==
	<StructureSection load='6tch' size='340' side='right'caption='[[6tch]]' scene=''>		<StructureSection load='6tch' size='340' side='right'caption='[[6tch]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TCH FirstGlance]. <br>		<table><tr><td colspan='2'>[[6tch]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TCH FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tch OCA], [https://pdbe.org/6tch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tch RCSB], [https://www.ebi.ac.uk/pdbsum/6tch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tch ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.801Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B3S:(3R)-3-AMINO-4-HYDROXYBUTANOIC+ACID'>B3S</scene>, <scene name='pdbligand=BAL:BETA-ALANINE'>BAL</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DLY:D-LYSINE'>DLY</scene>, <scene name='pdbligand=KCJ:3-(1,3-thiazol-4-yl)-L-alanine'>KCJ</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=NVA:NORVALINE'>NVA</scene>, <scene name='pdbligand=PPN:PARA-NITROPHENYLALANINE'>PPN</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tch OCA], [https://pdbe.org/6tch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tch RCSB], [https://www.ebi.ac.uk/pdbsum/6tch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tch ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			High-diversity genetically-encoded combinatorial libraries (10(8)-10(13) members) are a rich source of peptide-based binding molecules, identified by affinity selection. Synthetic libraries can access broader chemical space, but typically examine only ~ 10(6) compounds by screening. Here we show that in-solution affinity selection can be interfaced with nano-liquid chromatography-tandem mass spectrometry peptide sequencing to identify binders from fully randomized synthetic libraries of 10(8) members-a 100-fold gain in diversity over standard practice. To validate this approach, we show that binders to a monoclonal antibody are identified in proportion to library diversity, as diversity is increased from 10(6)-10(8). These results are then applied to the discovery of p53-like binders to MDM2, and to a family of 3-19 nM-affinity, alpha/beta-peptide-based binders to 14-3-3. An X-ray structure of one of these binders in complex with 14-3-3sigma is determined, illustrating the role of beta-amino acids in facilitating a key binding contact.

			Ultra-large chemical libraries for the discovery of high-affinity peptide binders.,Quartararo AJ, Gates ZP, Somsen BA, Hartrampf N, Ye X, Shimada A, Kajihara Y, Ottmann C, Pentelute BL Nat Commun. 2020 Jun 23;11(1):3183. doi: 10.1038/s41467-020-16920-3. PMID:32576815<ref>PMID:32576815</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6tch" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Synthetic construct]]
	[[Category: Ottmann C]]		[[Category: Ottmann C]]
	[[Category: Somsen BA]]		[[Category: Somsen BA]]

OCA at 08:56, 18 February 2021

2021-02-18T08:56:06Z

← Older revision		Revision as of 08:56, 18 February 2021
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	<StructureSection load='6tch' size='340' side='right'caption='[[6tch]]' scene=''>		<StructureSection load='6tch' size='340' side='right'caption='[[6tch]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TCH FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TCH FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tch OCA], [~~http~~://pdbe.org/6tch PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tch RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tch PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tch ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tch OCA], [https://pdbe.org/6tch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tch RCSB], [https://www.ebi.ac.uk/pdbsum/6tch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tch ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 14:34, 8 July 2020

2020-07-08T14:34:57Z

← Older revision		Revision as of 14:34, 8 July 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6tch is ~~ON HOLD until Paper Publication~~		==Binary complex of 14-3-3 sigma and a high-affinity non-canonical 9-mer peptide binder==
			<StructureSection load='6tch' size='340' side='right'caption='[[6tch]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TCH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TCH FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tch OCA], [http://pdbe.org/6tch PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tch RCSB], [http://www.ebi.ac.uk/pdbsum/6tch PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tch ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Ottmann C]]
			[[Category: Somsen BA]]

OCA at 13:36, 20 November 2019

2019-11-20T13:36:10Z

← Older revision		Revision as of 13:36, 20 November 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6tch is ON HOLD		The entry 6tch is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6tch" [edit=sysop:move=sysop]

2019-11-13T09:28:44Z

Protected "6tch" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:28, 13 November 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6tch is ON HOLD Authors: Description: Category: Unreleased Structures

2019-11-13T09:28:43Z

New page: '''Unreleased structure''' The entry 6tch is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tch is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]