6tip - Revision history

OCA at 13:03, 24 January 2024

2024-01-24T13:03:01Z

← Older revision		Revision as of 13:03, 24 January 2024
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	====		==Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide==
	<StructureSection load='6tip' size='340' side='right'caption='[[6tip]]' scene=''>		<StructureSection load='6tip' size='340' side='right'caption='[[6tip]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[6tip]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIP FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tip OCA], [https://pdbe.org/6tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tip RCSB], [https://www.ebi.ac.uk/pdbsum/6tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tip ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tip OCA], [https://pdbe.org/6tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tip RCSB], [https://www.ebi.ac.uk/pdbsum/6tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tip ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The affinity system based on the artificial peptide ligand Strep-tag(R) II and engineered tetrameric streptavidin, known as Strep-Tactin(R), offers attractive applications for the study of recombinant proteins, from detection and purification to functional immobilization. To further improve binding of the Strep-tag II to streptavidin we have subjected two protruding loops that shape its ligand pocket for the peptide - instead of D-biotin recognized by the natural protein - to iterative random mutagenesis. Sequence analyses of hits from functional screening assays revealed several unexpected structural motifs, such as a disulfide bridge at the base of one loop, replacement of the crucial residue Trp120 by Gly and a two-residue deletion in the second loop. The mutant m1-9 (dubbed Strep-Tactin XT) showed strongly enhanced affinity towards the Strep-tag II, which was further boosted in case of the bivalent Twin-Strep-tag(R). Four representative streptavidin mutants were crystallized in complex with the Strep-tag II peptide and their X-ray structures were solved at high resolutions. In addition, the crystal structure of the complex between Strep-Tactin XT and the Twin-Strep-tag was elucidated, indicating a bivalent mode of binding and explaining the experimentally observed avidity effect. Our study illustrates the structural plasticity of streptavidin as a scaffold for ligand binding and reveals interaction modes that would have been difficult to predict. As result, Strep-Tactin XT offers a convenient reagent for the kinetically stable immobilization of recombinant proteins fused with the Twin-Strep-tag. The possibility of reversibly dissociating such complexes simply with D-biotin as a competing ligand enables functional studies in protein science as well as cell biology.

			The Role of Changing Loop Conformations in Streptavidin Versions Engineered for High-affinity Binding of the Strep-tag II Peptide.,Schmidt TGM, Eichinger A, Schneider M, Bonet L, Carl U, Karthaus D, Theobald I, Skerra A J Mol Biol. 2021 Apr 30;433(9):166893. doi: 10.1016/j.jmb.2021.166893. Epub 2021 , Feb 24. PMID:33639211<ref>PMID:33639211</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6tip" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Avidin 3D structures\|Avidin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Streptomyces avidinii]]
			[[Category: Synthetic construct]]
			[[Category: Eichinger A]]
			[[Category: Skerra A]]

OCA at 06:52, 17 March 2021

2021-03-17T06:52:20Z

← Older revision		Revision as of 06:52, 17 March 2021
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	<StructureSection load='6tip' size='340' side='right'caption='[[6tip]]' scene=''>		<StructureSection load='6tip' size='340' side='right'caption='[[6tip]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tip OCA], [~~http~~://pdbe.org/6tip PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tip RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tip PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tip ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tip OCA], [https://pdbe.org/6tip PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tip RCSB], [https://www.ebi.ac.uk/pdbsum/6tip PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tip ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 07:43, 2 December 2020

2020-12-02T07:43:49Z

← Older revision		Revision as of 07:43, 2 December 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6tip ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='6tip' size='340' side='right'caption='[[6tip]]' scene=''>
	~~Authors~~: ~~Skerra, A~~.~~, Eichinger, A~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Engineered streptavidin variant (YNAFM) in complex with the Strep~~-~~tag II peptide~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tip FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tip OCA], [http://pdbe.org/6tip PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tip RCSB], [http://www.ebi.ac.uk/pdbsum/6tip PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tip ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Eichinger, A~~]]		__TOC__
	[[Category: ~~Skerra, A~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 06:16, 22 July 2020

2020-07-22T06:16:13Z

← Older revision		Revision as of 06:16, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6tip is ON HOLD		The entry 6tip is ON HOLD until Paper Publication

	Authors: Skerra, A., Eichinger, A.		Authors: Skerra, A., Eichinger, A.

OCA: Protected "6tip" [edit=sysop:move=sysop]

2019-12-04T06:25:06Z

Protected "6tip" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:25, 4 December 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6tip is ON HOLD Authors: Skerra, A., Eichinger, A. Description: Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide [[...

2019-12-04T06:25:04Z

New page: '''Unreleased structure''' The entry 6tip is ON HOLD Authors: Skerra, A., Eichinger, A. Description: Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide [[...

New page

'''Unreleased structure'''

The entry 6tip is ON HOLD

Authors: Skerra, A., Eichinger, A.

Description: Engineered streptavidin variant (YNAFM) in complex with the Strep-tag II peptide
[[Category: Unreleased Structures]]
[[Category: Eichinger, A]]
[[Category: Skerra, A]]