OCA at 13:09, 6 November 2024

2024-11-06T13:09:17Z

← Older revision		Revision as of 13:09, 6 November 2024
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	====		==Cryo-EM structure of Toxoplasma gondii mitochondrial ATP synthase hexamer, composite model==
	<StructureSection load='6tml' size='340' side='right'caption='[[6tml]]' scene=''>		<StructureSection load='6tml' size='340' side='right'caption='[[6tml]], [[Resolution\|resolution]] 4.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[6tml]] is a 138 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii_GT1 Toxoplasma gondii GT1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TML FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tml OCA], [~~http~~://pdbe.org/6tml PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tml RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tml PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tml ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.8Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tml OCA], [https://pdbe.org/6tml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tml RCSB], [https://www.ebi.ac.uk/pdbsum/6tml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tml ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/S7UQ82_TOXGG S7UQ82_TOXGG]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Mitochondrial ATP synthase plays a key role in inducing membrane curvature to establish cristae. In Apicomplexa causing diseases such as malaria and toxoplasmosis, an unusual cristae morphology has been observed, but its structural basis is unknown. Here, we report that the apicomplexan ATP synthase assembles into cyclic hexamers, essential to shape their distinct cristae. Cryo-EM was used to determine the structure of the hexamer, which is held together by interactions between parasite-specific subunits in the lumenal region. Overall, we identified 17 apicomplexan-specific subunits, and a minimal and nuclear-encoded subunit-a. The hexamer consists of three dimers with an extensive dimer interface that includes bound cardiolipins and the inhibitor IF(1). Cryo-ET and subtomogram averaging revealed that hexamers arrange into ~20-megadalton pentagonal pyramids in the curved apical membrane regions. Knockout of the linker protein ATPTG11 resulted in the loss of pentagonal pyramids with concomitant aberrantly shaped cristae. Together, this demonstrates that the unique macromolecular arrangement is critical for the maintenance of cristae morphology in Apicomplexa.

			ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria.,Muhleip A, Kock Flygaard R, Ovciarikova J, Lacombe A, Fernandes P, Sheiner L, Amunts A Nat Commun. 2021 Jan 5;12(1):120. doi: 10.1038/s41467-020-20381-z. PMID:33402698<ref>PMID:33402698</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6tml" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[ATPase 3D structures\|ATPase 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Toxoplasma gondii GT1]]
			[[Category: Amunts A]]
			[[Category: Kock Flygaard R]]
			[[Category: Muhleip A]]

OCA at 12:31, 16 December 2020

2020-12-16T12:31:02Z

← Older revision		Revision as of 12:31, 16 December 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6tml is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='6tml' size='340' side='right'caption='[[6tml]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tml OCA], [http://pdbe.org/6tml PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tml RCSB], [http://www.ebi.ac.uk/pdbsum/6tml PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tml ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 07:16, 25 December 2019

2019-12-25T07:16:17Z

← Older revision		Revision as of 07:16, 25 December 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6tml is ON HOLD		The entry 6tml is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6tml" [edit=sysop:move=sysop]

2019-12-11T13:39:26Z

Protected "6tml" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:39, 11 December 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6tml is ON HOLD Authors: Description: Category: Unreleased Structures

2019-12-11T13:39:24Z

New page: '''Unreleased structure''' The entry 6tml is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tml is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6tml - Revision history

OCA at 13:09, 6 November 2024

OCA at 12:31, 16 December 2020

OCA at 07:16, 25 December 2019

OCA: Protected "6tml" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6tml is ON HOLD Authors: Description: Category: Unreleased Structures