OCA at 13:05, 24 January 2024

2024-01-24T13:05:53Z

← Older revision		Revision as of 13:05, 24 January 2024
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	==Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).==		==Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).==
	<StructureSection load='6toc' size='340' side='right'caption='[[6toc]]' scene=''>		<StructureSection load='6toc' size='340' side='right'caption='[[6toc]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TOC FirstGlance]. <br>		<table><tr><td colspan='2'>[[6toc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TOC FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6toc OCA], [~~http~~://pdbe.org/6toc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6toc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6toc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6toc ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.853Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6toc OCA], [https://pdbe.org/6toc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6toc RCSB], [https://www.ebi.ac.uk/pdbsum/6toc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6toc ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PHR1_ARATH PHR1_ARATH] Transcription factor involved in phosphate starvation signaling (PubMed:11511543, PubMed:17927693, PubMed:26586833). Binds as a dimer to P1BS, an imperfect palindromic sequence 5'-GNATATNC-3', to promote the expression of inorganic phosphate (Pi) starvation-responsive genes (PubMed:11511543, PubMed:20838596, PubMed:26586833). SPX1 is a competitive inhibitor of this DNA-binding (PubMed:25271326). PHR1 binding to its targets is low Pi-dependent (PubMed:25271326). Regulates the expression of miR399 (PubMed:20838596). Regulates the expression of IPS1 (At3g09922), a non-coding RNA that mimics the target of miR399 to block the cleavage of PHO2 under Pi-deficient conditions (PubMed:17643101). Regulates lipid remodeling and triacylglycerol accumulation during phosphorus starvation (PubMed:25680792). Required for the shoot-specific hypoxic response (PubMed:24753539). Regulates FER1 expression upon phosphate starvation, linking iron and phosphate homeostasis (PubMed:23788639). Contributes to the homeostasis of both sulfate and phosphate in plants under phosphate deficiency (PubMed:21261953). Required for adaptation to high light and retaining functional photosynthesis during phosphate starvation (PubMed:21910737). Involved in the coregulation of Zn and Pi homeostasis (PubMed:24420568).<ref>PMID:11511543</ref> <ref>PMID:17643101</ref> <ref>PMID:17927693</ref> <ref>PMID:20838596</ref> <ref>PMID:21261953</ref> <ref>PMID:21910737</ref> <ref>PMID:23788639</ref> <ref>PMID:24420568</ref> <ref>PMID:24753539</ref> <ref>PMID:25271326</ref> <ref>PMID:25680792</ref> <ref>PMID:26586833</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Phosphorus is an essential nutrient taken up by organisms in the form of inorganic phosphate (Pi). Eukaryotes have evolved sophisticated Pi sensing and signaling cascades, enabling them to stably maintain cellular Pi concentrations. Pi homeostasis is regulated by inositol pyrophosphate signaling molecules (PP-InsPs), which are sensed by SPX domain-containing proteins. In plants, PP-InsP-bound SPX receptors inactivate Myb coiled-coil (MYB-CC) Pi starvation response transcription factors (PHRs) by an unknown mechanism. Here we report that a InsP(8)-SPX complex targets the plant-unique CC domain of PHRs. Crystal structures of the CC domain reveal an unusual four-stranded anti-parallel arrangement. Interface mutations in the CC domain yield monomeric PHR1, which is no longer able to bind DNA with high affinity. Mutation of conserved basic residues located at the surface of the CC domain disrupt interaction with the SPX receptor in vitro and in planta, resulting in constitutive Pi starvation responses. Together, our findings suggest that InsP(8) regulates plant Pi homeostasis by controlling the oligomeric state and hence the promoter binding capability of PHRs via their SPX receptors.

			Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis.,Ried MK, Wild R, Zhu J, Pipercevic J, Sturm K, Broger L, Harmel RK, Abriata LA, Hothorn LA, Fiedler D, Hiller S, Hothorn M Nat Commun. 2021 Jan 15;12(1):384. doi: 10.1038/s41467-020-20681-4. PMID:33452263<ref>PMID:33452263</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6toc" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hothorn M]]		[[Category: Hothorn M]]

OCA at 05:47, 20 January 2021

2021-01-20T05:47:50Z

← Older revision		Revision as of 05:47, 20 January 2021
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	~~'''Unreleased structure'''~~

	~~The entry 6toc is ON HOLD until Paper Publication~~		==Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).==
			<StructureSection load='6toc' size='340' side='right'caption='[[6toc]]' scene=''>
	~~Authors: Hothorn, M.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TOC FirstGlance]. <br>
	~~Description:~~ Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6toc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6toc OCA], [http://pdbe.org/6toc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6toc RCSB], [http://www.ebi.ac.uk/pdbsum/6toc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6toc ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Hothorn, M]]		__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Hothorn M]]

OCA: Protected "6toc" [edit=sysop:move=sysop]

2019-12-18T05:55:10Z

Protected "6toc" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:55, 18 December 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6toc is ON HOLD until Paper Publication Authors: Hothorn, M. Description: Crystal structure of the oligomerisation domain of the transcription fact...

2019-12-18T05:55:08Z

New page: '''Unreleased structure''' The entry 6toc is ON HOLD until Paper Publication Authors: Hothorn, M. Description: Crystal structure of the oligomerisation domain of the transcription fact...

New page

'''Unreleased structure'''

The entry 6toc is ON HOLD until Paper Publication

Authors: Hothorn, M.

Description: Crystal structure of the oligomerisation domain of the transcription factor PHOSPHATE STARVATION RESPONSE 1 from Arabidopsis (crystal form 3).
[[Category: Unreleased Structures]]
[[Category: Hothorn, M]]

6toc - Revision history

OCA at 13:05, 24 January 2024

OCA at 05:47, 20 January 2021

OCA: Protected "6toc" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6toc is ON HOLD until Paper Publication Authors: Hothorn, M. Description: Crystal structure of the oligomerisation domain of the transcription fact...