OCA at 08:08, 11 October 2023

2023-10-11T08:08:23Z

← Older revision		Revision as of 08:08, 11 October 2023
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	==Crystal structure of N-terminal domain of Mycobacterium tuberculosis complex Lon protease==		==Crystal structure of N-terminal domain of Mycobacterium tuberculosis complex Lon protease==
	<StructureSection load='6vbk' size='340' side='right'caption='[[6vbk]]' scene=''>		<StructureSection load='6vbk' size='340' side='right'caption='[[6vbk]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6VBK FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vbk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VBK FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbk OCA], [~~http~~://pdbe.org/6vbk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vbk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vbk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbk ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbk OCA], [https://pdbe.org/6vbk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vbk RCSB], [https://www.ebi.ac.uk/pdbsum/6vbk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbk ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Lon protease is evolutionarily conserved in prokaryotes and eukaryotic organelles. The primary function of Lon is to selectively degrade abnormal and certain regulatory proteins to maintain the homeostasis in vivo. Lon mainly consists of three functional domains and the N-terminal domain is required for the substrate selection and recognition. However, the precise contribution of the N-terminal domain remains elusive. Here, we determined the crystal structure of the N-terminal 192-residue construct of Lon protease from Mycobacterium avium complex at 2.4 a resolutionand measured NMR-relaxation parameters of backbones. This structure consists of two subdomains, the beta-strand rich N-terminal subdomain and the five-helix bundle of C-terminal subdomain, connected by a flexible linkerand is similar to the overall structure of the N domain of E. coli Lon even though their sequence identity is only 26%. The obtained NMR-relaxation parameters reveal two stabilized loops involving in the structural packing of the compact N domain and a turn structure formation. The performed homology comparison suggests that structural and sequence variations in the N domain may be closely related to the substrate selectivity of Lon variants. Our results provide the structure and dynamics characterization of a new Lon N domain, and will help to define the precise contribution of the Lon N-terminal domain to the substrate recognition.

			Crystal structure of the N domain of Lon protease from Mycobacterium avium complex.,Chen X, Zhang S, Bi F, Guo C, Feng L, Wang H, Yao H, Lin D Protein Sci. 2019 Jul 15. doi: 10.1002/pro.3687. PMID:31306520<ref>PMID:31306520</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6vbk" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Mycobacterium tuberculosis]]
	[[Category: Bi FK]]		[[Category: Bi FK]]
	[[Category: Chen C]]		[[Category: Chen C]]

OCA at 10:29, 24 December 2020

2020-12-24T10:29:00Z

← Older revision		Revision as of 10:29, 24 December 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6vbk is ~~ON HOLD until Paper Publication~~		==Crystal structure of N-terminal domain of Mycobacterium tuberculosis complex Lon protease==
			<StructureSection load='6vbk' size='340' side='right'caption='[[6vbk]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VBK FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vbk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbk OCA], [http://pdbe.org/6vbk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vbk RCSB], [http://www.ebi.ac.uk/pdbsum/6vbk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbk ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Bi FK]]
			[[Category: Chen C]]
			[[Category: Chen XY]]
			[[Category: Guo CY]]
			[[Category: Lin DH]]

OCA at 06:48, 1 January 2020

2020-01-01T06:48:32Z

← Older revision		Revision as of 06:48, 1 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6vbk is ON HOLD		The entry 6vbk is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6vbk" [edit=sysop:move=sysop]

2019-12-25T07:39:34Z

Protected "6vbk" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:39, 25 December 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6vbk is ON HOLD Authors: Description: Category: Unreleased Structures

2019-12-25T07:39:32Z

New page: '''Unreleased structure''' The entry 6vbk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6vbk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6vbk - Revision history

OCA at 08:08, 11 October 2023

OCA at 10:29, 24 December 2020

OCA at 06:48, 1 January 2020

OCA: Protected "6vbk" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6vbk is ON HOLD Authors: Description: Category: Unreleased Structures