6vbv - Revision history

OCA at 14:37, 6 March 2024

2024-03-06T14:37:02Z

← Older revision		Revision as of 14:37, 6 March 2024
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	<SX load='6vbv' size='340' side='right' viewer='molstar' caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='6vbv' size='340' side='right' viewer='molstar' caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6vbv]] is a 8 chain structure with sequence from [~~http://en.wikipedia.org/wiki/ ] and [http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6VBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vbv]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VBV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbv OCA], [~~http~~://pdbe.org/6vbv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vbv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vbv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbv ProSAT]</span></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbv OCA], [https://pdbe.org/6vbv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vbv RCSB], [https://www.ebi.ac.uk/pdbsum/6vbv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[~~[http~~://www.uniprot.org/uniprot/~~F1MB52_BOVIN F1MB52_BOVIN~~]] The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function.[PIRNR:PIRNR011091] [[http://www.uniprot.org/uniprot/ARL6_BOVIN ARL6_BOVIN]] Involved in membrane protein trafficking at the base of the ciliary organelle. Mediates recruitment onto plasma membrane of the BBSome complex which would constitute a coat complex required for sorting of specific membrane proteins to the primary cilia. Together with the BBSome complex and LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. May regulate cilia assembly and disassembly and subsequent ciliary signaling events such as the Wnt signaling cascade. Isoform 2 may be required for proper retinal function and organization (By similarity). [[http://www.uniprot.org/uniprot/BBS4_BOVIN BBS4_BOVIN]] May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane (By similarity). [[http://www.uniprot.org/uniprot/A6QLF9_BOVIN A6QLF9_BOVIN]] The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.[PIRNR:PIRNR010072]		[https://www.uniprot.org/uniprot/G3N2W1_BOVIN G3N2W1_BOVIN]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Bardet-Biedl syndrome (BBS) is a currently incurable ciliopathy caused by the failure to correctly establish or maintain cilia-dependent signaling pathways. Eight proteins associated with BBS assemble into the BBSome, a key regulator of the ciliary membrane proteome. We report the electron cryomicroscopy (cryo-EM) structures of the native bovine BBSome in inactive and active states at 3.1 -and 3.5 A resolution, respectively. In the active state, the BBSome is bound to an Arf-family GTPase (ARL6/BBS3) that recruits the BBSome to ciliary membranes. ARL6 recognizes a composite binding site formed by BBS1 and BBS7 that is occluded in the inactive state. Activation requires an unexpected swiveling of the b-propeller domain of BBS1, the subunit most frequently implicated in substrate recognition, which widens a central cavity of the BBSome. Structural mapping of disease-causing mutations suggests that pathogenesis results from folding defects and the disruption of autoinhibition and activation.

	Structure and activation mechanism of the BBSome membrane protein trafficking complex.,Singh SK, Gui M, Koh F, Yip MC, Brown A Elife. 2020 Jan 15;9. pii: 53322. doi: 10.7554/eLife.53322. PMID:31939736<ref>PMID:31939736</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 6vbv" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</SX>		</SX>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brown, A]]		[[Category: Brown A]]
	[[Category: Gui, M]]		[[Category: Gui M]]
	[[Category: Koh, F]]		[[Category: Koh F]]
	[[Category: Singh~~, S K~~]]		[[Category: Singh SK]]
	[[Category: Yip~~, M C.J]]~~		[[Category: Yip MCJ]]
	~~[[Category: Cilia]]~~
	~~[[Category: Ciliopathy]]~~
	~~[[Category: Complex]]~~
	~~[[Category: Membrane-protein transport]]~~
	~~[[Category: Protein transport~~]]

OCA at 07:07, 11 April 2020

2020-04-11T07:07:30Z

← Older revision		Revision as of 07:07, 11 April 2020
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	<SX load='6vbv' size='340' side='right' viewer='molstar' caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='6vbv' size='340' side='right' viewer='molstar' caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6vbv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6VBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vbv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VBV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=6vbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbv OCA], [http://pdbe.org/6vbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vbv RCSB], [http://www.ebi.ac.uk/pdbsum/6vbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbv ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vbv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vbv OCA], [http://pdbe.org/6vbv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vbv RCSB], [http://www.ebi.ac.uk/pdbsum/6vbv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vbv ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:25, 7 March 2020

2020-03-07T01:25:09Z

← Older revision		Revision as of 01:25, 7 March 2020
Line 1:		Line 1:

	==Structure of the bovine BBSome:ARL6:GTP complex==		==Structure of the bovine BBSome:ARL6:GTP complex==
	<~~StructureSection~~ load='6vbv' size='340' side='right'caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>		<SX load='6vbv' size='340' side='right' viewer='molstar' caption='[[6vbv]], [[Resolution\|resolution]] 3.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6vbv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VBV FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vbv]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VBV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VBV FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
	</~~StructureSection~~>		</SX>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 15:47, 29 January 2020

2020-01-29T15:47:39Z

@@ Line 1: / Line 1: @@
-The entry 6vbv is ON HOLD
+==Structure of the bovine BBSome:ARL6:GTP complex==
-Authors: Singh, S.K., Gui, M., Koh, F., Yip, M.C.J., Brown, A.
+Structure and activation mechanism of the BBSome membrane protein trafficking complex.,Singh SK, Gui M, Koh F, Yip MC, Brown A Elife. 2020 Jan 15;9. pii: 53322. doi: 10.7554/eLife.53322. PMID:31939736<ref>PMID:31939736</ref>
-Description: Structure of the bovine BBSome:ARL6:GTP complex
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
 [[Category: Brown, A]]
 [[Category: Gui, M]]
 [[Category: Koh, F]]
-[[Category: Singh, S.K]]
+[[Category: Singh, S K]]

OCA: Protected "6vbv" [edit=sysop:move=sysop]

2019-12-25T07:40:35Z

Protected "6vbv" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:40, 25 December 2019
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6vbv is ON HOLD Authors: Singh, S.K., Gui, M., Koh, F., Yip, M.C.J., Brown, A. Description: Structure of the bovine BBSome:ARL6:GTP complex [[Categ...

2019-12-25T07:40:33Z

New page: '''Unreleased structure''' The entry 6vbv is ON HOLD Authors: Singh, S.K., Gui, M., Koh, F., Yip, M.C.J., Brown, A. Description: Structure of the bovine BBSome:ARL6:GTP complex [[Categ...

New page

'''Unreleased structure'''

The entry 6vbv is ON HOLD

Authors: Singh, S.K., Gui, M., Koh, F., Yip, M.C.J., Brown, A.

Description: Structure of the bovine BBSome:ARL6:GTP complex
[[Category: Unreleased Structures]]
[[Category: Brown, A]]
[[Category: Yip, M.C.J]]
[[Category: Gui, M]]
[[Category: Koh, F]]
[[Category: Singh, S.K]]