6vr2 - Revision history

OCA at 08:17, 11 October 2023

2023-10-11T08:17:45Z

← Older revision		Revision as of 08:17, 11 October 2023
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	<StructureSection load='6vr2' size='340' side='right'caption='[[6vr2]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='6vr2' size='340' side='right'caption='[[6vr2]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6vr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"proteus_stuartii"_buttiaux_et_al._1954 "proteus~~ stuartii~~" buttiaux et al. 1954~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Providencia_stuartii Providencia stuartii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VR2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=R7Y:(1S,2S,3R,4S,6R)-3-{[2-(acetylamino)-6-amino-2,3,6-trideoxy-alpha-D-ribo-hexopyranosyl]oxy}-4,6-diamino-2-hydroxycycloh+exyl+3-amino-3-deoxy-alpha-D-glucopyranoside'>R7Y</scene~~></td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
	~~<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vou\|6vou]]</div></td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=R7Y:(1S,2S,3R,4S,6R)-3-{[2-(acetylamino)-6-amino-2,3,6-trideoxy-alpha-D-ribo-hexopyranosyl]oxy}-4,6-diamino-2-hydroxycycloh+exyl+3-amino-3-deoxy-alpha-D-glucopyranoside'>R7Y</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588 "Proteus stuartii" Buttiaux et al. 1954])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Gentamicin_2'-N-acetyltransferase Gentamicin 2'-N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.59 2.3.1.59] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vr2 OCA], [https://pdbe.org/6vr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vr2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vr2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vr2 OCA], [https://pdbe.org/6vr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vr2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vr2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/AAC2_PROST AAC2_PROST]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Proteus stuartii buttiaux et al. 1954]]~~
	~~[[Category: Gentamicin 2'-N-acetyltransferase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bassenden, A V~~]]		[[Category: Providencia stuartii]]
	[[Category: ~~Berghuis, A M~~]]		[[Category: Bassenden AV]]
	[[Category: ~~Acetyltransferase]]~~		[[Category: Berghuis AM]]
	~~[[Category: Gnat superfamily]]~~
	~~[[Category: Transferase~~]]

OCA at 09:59, 14 July 2021

2021-07-14T09:59:45Z

← Older revision		Revision as of 09:59, 14 July 2021
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	==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-tobramycin and CoA==		==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-tobramycin and CoA==
	<StructureSection load='6vr2' size='340' side='right'caption='[[6vr2]]' scene=''>		<StructureSection load='6vr2' size='340' side='right'caption='[[6vr2]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VR2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6vr2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"proteus_stuartii"_buttiaux_et_al._1954 "proteus stuartii" buttiaux et al. 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VR2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vr2 OCA], [https://pdbe.org/6vr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vr2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vr2 ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=R7Y:(1S,2S,3R,4S,6R)-3-{[2-(acetylamino)-6-amino-2,3,6-trideoxy-alpha-D-ribo-hexopyranosyl]oxy}-4,6-diamino-2-hydroxycycloh+exyl+3-amino-3-deoxy-alpha-D-glucopyranoside'>R7Y</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6vou\|6vou]]</div></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">aac ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=588 "Proteus stuartii" Buttiaux et al. 1954])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Gentamicin_2'-N-acetyltransferase Gentamicin 2'-N-acetyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.59 2.3.1.59] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vr2 OCA], [https://pdbe.org/6vr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vr2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vr2 ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Plazomicin is currently the only next-generation aminoglycoside approved for clinical use that has the potential of evading the effects of widespread enzymatic resistance factors. However, plazomicin is still susceptible to the action of the resistance enzyme AAC(2')-Ia from Providencia stuartii. As the clinical use of plazomicin begins to increase, the spread of resistance factors will undoubtedly accelerate, rendering this aminoglycoside increasingly obsolete. Understanding resistance to plazomicin is an important step to ensure this aminoglycoside remains a viable treatment option for the foreseeable future. Here, we present three crystal structures of AAC(2')-Ia from P. stuartii, two in complex with acetylated aminoglycosides tobramycin and netilmicin, and one in complex with a non-substrate aminoglycoside, amikacin. Together, with our previously reported AAC(2')-Ia-acetylated plazomicin complex, these structures outline AAC(2')-Ia's specificity for a wide range of aminoglycosides. Additionally, our survey of AAC(2')-I homologues highlights the conservation of residues predicted to be involved in aminoglycoside binding, and identifies the presence of plasmid-encoded enzymes in environmental strains that confer resistance to the latest next-generation aminoglycoside. These results forecast the likely spread of plazomicin resistance and highlight the urgency for advancements in next-generation aminoglycoside design.

			Structural and phylogenetic analyses of resistance to next-generation aminoglycosides conferred by AAC(2') enzymes.,Bassenden AV, Dumalo L, Park J, Blanchet J, Maiti K, Arya DP, Berghuis AM Sci Rep. 2021 Jun 2;11(1):11614. doi: 10.1038/s41598-021-89446-3. PMID:34078922<ref>PMID:34078922</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6vr2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Proteus stuartii buttiaux et al. 1954]]
			[[Category: Gentamicin 2'-N-acetyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bassenden AV]]		[[Category: Bassenden, A V]]
	[[Category: Berghuis AM]]		[[Category: Berghuis, A M]]
			[[Category: Acetyltransferase]]
			[[Category: Gnat superfamily]]
			[[Category: Transferase]]

OCA at 14:32, 2 June 2021

2021-06-02T14:32:22Z

← Older revision		Revision as of 14:32, 2 June 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6vr2 is ~~ON HOLD until Paper Publication~~		==Aminoglycoside N-2'-Acetyltransferase-Ia [AAC(2')-Ia] in complex with acetylated-tobramycin and CoA==
			<StructureSection load='6vr2' size='340' side='right'caption='[[6vr2]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VR2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VR2 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vr2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vr2 OCA], [https://pdbe.org/6vr2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vr2 RCSB], [https://www.ebi.ac.uk/pdbsum/6vr2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vr2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Bassenden AV]]
			[[Category: Berghuis AM]]

OCA at 08:08, 26 February 2020

2020-02-26T08:08:01Z

← Older revision		Revision as of 08:08, 26 February 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6vr2 is ON HOLD		The entry 6vr2 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6vr2" [edit=sysop:move=sysop]

2020-02-13T02:50:41Z

Protected "6vr2" [edit=sysop:move=sysop]

← Older revision	Revision as of 02:50, 13 February 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6vr2 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-02-13T02:50:40Z

New page: '''Unreleased structure''' The entry 6vr2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6vr2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]