OCA at 14:25, 18 October 2023

2023-10-18T14:25:34Z

← Older revision		Revision as of 14:25, 18 October 2023
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	====		==Nucleotide incorporation intermediate into quaternary complex of human Polymerase Mu on a complementary DNA double-strand break substrate==
	<StructureSection load='6wid' size='340' side='right'caption='[[6wid]]' scene=''>		<StructureSection load='6wid' size='340' side='right'caption='[[6wid]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[6wid]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WID OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WID FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wid OCA], [~~http~~://pdbe.org/6wid PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wid RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wid PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wid ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.5Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=DUP:2-DEOXYURIDINE+5-ALPHA,BETA-IMIDO-TRIPHOSPHATE'>DUP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PPV:PYROPHOSPHATE'>PPV</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wid OCA], [https://pdbe.org/6wid PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wid RCSB], [https://www.ebi.ac.uk/pdbsum/6wid PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wid ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/DPOLM_HUMAN DPOLM_HUMAN] Gap-filling polymerase involved in repair of DNA double-strand breaks by non-homologous end joining (NHEJ). Participates in immunoglobulin (Ig) light chain gene rearrangement in V(D)J recombination.<ref>PMID:12640116</ref> <ref>PMID:12888504</ref> <ref>PMID:17483519</ref> <ref>PMID:17915942</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Genomic integrity is threatened by cytotoxic DNA double-strand breaks (DSBs), which must be resolved efficiently to prevent sequence loss, chromosomal rearrangements/translocations, or cell death. Polymerase mu (Polmu) participates in DSB repair via the nonhomologous end-joining (NHEJ) pathway, by filling small sequence gaps in broken ends to create substrates ultimately ligatable by DNA Ligase IV. Here we present structures of human Polmu engaging a DSB substrate. Synapsis is mediated solely by Polmu, facilitated by single-nucleotide homology at the break site, wherein both ends of the discontinuous template strand are stabilized by a hydrogen bonding network. The active site in the quaternary Pol mu complex is poised for catalysis and nucleotide incoporation proceeds in crystallo. These structures demonstrate that Polmu may address complementary DSB substrates during NHEJ in a manner indistinguishable from single-strand breaks.

			Structural snapshots of human DNA polymerase mu engaged on a DNA double-strand break.,Kaminski AM, Pryor JM, Ramsden DA, Kunkel TA, Pedersen LC, Bebenek K Nat Commun. 2020 Sep 22;11(1):4784. doi: 10.1038/s41467-020-18506-5. PMID:32963245<ref>PMID:32963245</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6wid" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Synthetic construct]]
			[[Category: Bebenek K]]
			[[Category: Kaminski AM]]
			[[Category: Kunkel TA]]
			[[Category: Pedersen LC]]

OCA at 06:19, 7 October 2020

2020-10-07T06:19:32Z

← Older revision		Revision as of 06:19, 7 October 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6wid is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='6wid' size='340' side='right'caption='[[6wid]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wid FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wid OCA], [http://pdbe.org/6wid PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wid RCSB], [http://www.ebi.ac.uk/pdbsum/6wid PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wid ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 04:15, 22 April 2020

2020-04-22T04:15:16Z

← Older revision		Revision as of 04:15, 22 April 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6wid is ON HOLD		The entry 6wid is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wid" [edit=sysop:move=sysop]

2020-04-15T05:29:47Z

Protected "6wid" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:29, 15 April 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6wid is ON HOLD Authors: Description: Category: Unreleased Structures

2020-04-15T05:29:46Z

New page: '''Unreleased structure''' The entry 6wid is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wid is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wid - Revision history

OCA at 14:25, 18 October 2023

OCA at 06:19, 7 October 2020

OCA at 04:15, 22 April 2020

OCA: Protected "6wid" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wid is ON HOLD Authors: Description: Category: Unreleased Structures