6wp2 - Revision history

OCA at 14:32, 18 October 2023

2023-10-18T14:32:26Z

← Older revision		Revision as of 14:32, 18 October 2023
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	<StructureSection load='6wp2' size='340' side='right'caption='[[6wp2]], [[Resolution\|resolution]] 2.48Å' scene=''>		<StructureSection load='6wp2' size='340' side='right'caption='[[6wp2]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6wp2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6wp2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WP2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.48Å</td></tr>
	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBP2, CRBP2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp2 OCA], [https://pdbe.org/6wp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6wp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp2 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp2 OCA], [https://pdbe.org/6wp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6wp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN]] Intracellular transport of retinol.		[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN] Intracellular transport of retinol.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Geiger, J]]		[[Category: Geiger J]]
	[[Category: Ghanbarpour, A]]		[[Category: Ghanbarpour A]]
	~~[[Category: Ilbp]]~~
	~~[[Category: Lipid binding protein~~]]

OCA at 08:59, 5 May 2021

2021-05-05T08:59:23Z

← Older revision		Revision as of 08:59, 5 May 2021
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	==The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H Variant of HCRBPII==		==The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H Variant of HCRBPII==
	<StructureSection load='6wp2' size='340' side='right'caption='[[6wp2]]' scene=''>		<StructureSection load='6wp2' size='340' side='right'caption='[[6wp2]], [[Resolution\|resolution]] 2.48Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WP2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6wp2]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WP2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp2 OCA], [~~http~~://pdbe.org/6wp2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wp2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wp2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp2 ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">RBP2, CRBP2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp2 OCA], [https://pdbe.org/6wp2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wp2 RCSB], [https://www.ebi.ac.uk/pdbsum/6wp2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/RET2_HUMAN RET2_HUMAN]] Intracellular transport of retinol.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Domain-swapping is a mechanism for evolving new protein structure from extant scaffolds, and has been an efficient protein-engineering strategy for tailoring functional diversity. However, domain swapping can only be exploited if it can be controlled, especially in cases where various folds can coexist. Herein, we describe the structure of a domain-swapped trimer of the iLBP family member hCRBPII, and suggest a mechanism for domain-swapped trimerization. It is further shown that domain-swapped trimerization can be favored by strategic installation of a disulfide bond, thus demonstrating a strategy for fold control. We further show the domain-swapped trimer to be a useful protein design template by installing a high-affinity metal binding site through the introduction of a single mutation, taking advantage of its threefold symmetry. Together, these studies show how nature can promote oligomerization, stabilize a specific oligomer, and generate new function with minimal changes to the protein sequence.

			Human Cellular Retinol Binding Protein II Forms a Domain-Swapped Trimer Representing a Novel Fold and a New Template for Protein Engineering.,Ghanbarpour A, Santos EM, Pinger C, Assar Z, Hossaini Nasr S, Vasileiou C, Spence D, Borhan B, Geiger JH Chembiochem. 2020 Jun 30. doi: 10.1002/cbic.202000405. PMID:32608180<ref>PMID:32608180</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6wp2" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Retinol-binding protein 3D structures\|Retinol-binding protein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Geiger J]]		[[Category: Geiger, J]]
	[[Category: Ghanbarpour A]]		[[Category: Ghanbarpour, A]]
			[[Category: Ilbp]]
			[[Category: Lipid binding protein]]

OCA at 06:22, 19 August 2020

2020-08-19T06:22:03Z

← Older revision		Revision as of 06:22, 19 August 2020
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	~~'''Unreleased structure'''~~

	~~The entry 6wp2 is ON HOLD until Paper Publication~~		==The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H Variant of HCRBPII==
			<StructureSection load='6wp2' size='340' side='right'caption='[[6wp2]]' scene=''>
	~~Authors: Ghanbarpour, A., Geiger, J.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WP2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WP2 FirstGlance]. <br>
	~~Description:~~ The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H ~~variant~~ of HCRBPII		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wp2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wp2 OCA], [http://pdbe.org/6wp2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wp2 RCSB], [http://www.ebi.ac.uk/pdbsum/6wp2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wp2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Ghanbarpour, A~~]]		__TOC__
	[[Category: ~~Geiger, J~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Geiger J]]
			[[Category: Ghanbarpour A]]

OCA at 04:14, 20 May 2020

2020-05-20T04:14:33Z

← Older revision		Revision as of 04:14, 20 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6wp2 is ON HOLD		The entry 6wp2 is ON HOLD until Paper Publication

	Authors: Ghanbarpour, A., Geiger, J.		Authors: Ghanbarpour, A., Geiger, J.

OCA: Protected "6wp2" [edit=sysop:move=sysop]

2020-05-06T04:28:03Z

Protected "6wp2" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:28, 6 May 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6wp2 is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58...

2020-05-06T04:28:02Z

New page: '''Unreleased structure''' The entry 6wp2 is ON HOLD Authors: Ghanbarpour, A., Geiger, J. Description: The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58...

New page

'''Unreleased structure'''

The entry 6wp2 is ON HOLD

Authors: Ghanbarpour, A., Geiger, J.

Description: The Crystal Structure of Apo Zinc-Bound Domain Swapped-Trimer Q108K:K40D:T53A:R58L:Q38F:Q4F:F57H variant of HCRBPII
[[Category: Unreleased Structures]]
[[Category: Ghanbarpour, A]]
[[Category: Geiger, J]]