6xml - Revision history

OCA at 14:56, 18 October 2023

2023-10-18T14:56:02Z

← Older revision		Revision as of 14:56, 18 October 2023
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	<StructureSection load='6xml' size='340' side='right'caption='[[6xml]], [[Resolution\|resolution]] 1.88Å' scene=''>		<StructureSection load='6xml' size='340' side='right'caption='[[6xml]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6xml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XML FirstGlance]. <br>		<table><tr><td colspan='2'>[[6xml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XML FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.88Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ALDOA, ALDA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~9606 HUMAN])</td~~><~~/tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xml OCA], [https://pdbe.org/6xml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xml RCSB], [https://www.ebi.ac.uk/pdbsum/6xml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xml ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xml OCA], [https://pdbe.org/6xml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xml RCSB], [https://www.ebi.ac.uk/pdbsum/6xml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xml ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN]] Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:[https://omim.org/entry/611881 611881]]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.<ref>PMID:14766013</ref> <ref>PMID:2825199</ref> <ref>PMID:2229018</ref> <ref>PMID:8598869</ref> <ref>PMID:14615364</ref>		[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN] Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:[https://omim.org/entry/611881 611881]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.<ref>PMID:14766013</ref> <ref>PMID:2825199</ref> <ref>PMID:2229018</ref> <ref>PMID:8598869</ref> <ref>PMID:14615364</ref>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).		[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Fructose-bisphosphate aldolase]]~~		[[Category: Homo sapiens]]
	~~[[Category: Human~~]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brewer, K]]		[[Category: Brewer K]]
	[[Category: Lamb~~, A L~~]]		[[Category: Lamb AL]]
	[[Category: Meneely~~, K M]]~~		[[Category: Meneely KM]]
	~~[[Category: Allosterism]]~~
	~~[[Category: Lyase]]~~
	~~[[Category: Rheostat~~]]

OCA at 07:34, 19 January 2022

2022-01-19T07:34:41Z

← Older revision		Revision as of 07:34, 19 January 2022
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	==Human aldolase A I98C==		==Human aldolase A I98C==
	<StructureSection load='6xml' size='340' side='right'caption='[[6xml]]' scene=''>		<StructureSection load='6xml' size='340' side='right'caption='[[6xml]], [[Resolution\|resolution]] 1.88Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XML FirstGlance]. <br>		<table><tr><td colspan='2'>[[6xml]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XML FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xml OCA], [https://pdbe.org/6xml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xml RCSB], [https://www.ebi.ac.uk/pdbsum/6xml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xml ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ALDOA, ALDA ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Fructose-bisphosphate_aldolase Fructose-bisphosphate aldolase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.13 4.1.2.13] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xml OCA], [https://pdbe.org/6xml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xml RCSB], [https://www.ebi.ac.uk/pdbsum/6xml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xml ProSAT]</span></td></tr>
	</table>		</table>
			== Disease ==
			[[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN]] Defects in ALDOA are the cause of glycogen storage disease type 12 (GSD12) [MIM:[https://omim.org/entry/611881 611881]]; also known as red cell aldolase deficiency. A metabolic disorder associated with increased hepatic glycogen and hemolytic anemia. It may lead to myopathy with exercise intolerance and rhabdomyolysis.<ref>PMID:14766013</ref> <ref>PMID:2825199</ref> <ref>PMID:2229018</ref> <ref>PMID:8598869</ref> <ref>PMID:14615364</ref>
			== Function ==
			[[https://www.uniprot.org/uniprot/ALDOA_HUMAN ALDOA_HUMAN]] Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein (By similarity).
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Some protein positions play special roles in determining the magnitude of protein function: at such "rheostat" positions, varied amino acid substitutions give rise to a continuum of functional outcomes, from wild type (or enhanced), to intermediate, to loss of function. This observed range raises interesting questions about the biophysical bases by which changes at single positions have such varied outcomes. Here, we assessed variants at position 98 in human aldolase A ("I98X"). Despite being ~17 A from the active site and far from subunit interfaces, substitutions at position 98 have rheostatic contributions to the apparent cooperativity (nH ) associated with fructose-1,6-bisphosphate substrate binding and moderately affected binding affinity. Next, we crystallized representative I98X variants to assess structural consequences. Residues smaller than the native isoleucine (cysteine and serine) were readily accommodated, and the larger phenylalanine caused only a slight separation of the two parallel helixes. However, the diffraction quality was reduced for I98F, and further reduced for I98Y. Intriguingly, the resolutions of the I98X structures correlated with their nH values. We propose that substitution effects on both nH and crystal lattice disruption arise from changes in the population of aldolase A conformations in solution. In combination with results computed for rheostat positions in other proteins, the results from this study suggest that rheostat positions accommodate a wide range of side chains and that structural consequences manifest as shifted ensemble populations and/or dynamics changes.

			Substitutions at a rheostat position in human aldolase A cause a shift in the conformational population.,Fenton KD, Meneely KM, Wu T, Martin TA, Swint-Kruse L, Fenton AW, Lamb AL Protein Sci. 2021 Nov 4. doi: 10.1002/pro.4222. PMID:34734672<ref>PMID:34734672</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 6xml" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Fructose-bisphosphate aldolase]]
			[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brewer K]]		[[Category: Brewer, K]]
	[[Category: Lamb AL]]		[[Category: Lamb, A L]]
	[[Category: Meneely KM]]		[[Category: Meneely, K M]]
			[[Category: Allosterism]]
			[[Category: Lyase]]
			[[Category: Rheostat]]

OCA at 09:53, 7 July 2021

2021-07-07T09:53:22Z

← Older revision		Revision as of 09:53, 7 July 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 6xml is ~~ON HOLD until Paper Publication~~		==Human aldolase A I98C==
			<StructureSection load='6xml' size='340' side='right'caption='[[6xml]]' scene=''>
	~~Authors~~: ~~Meneely~~, K.M., ~~Brewer~~, K., ~~Lamb~~, A.L.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XML OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XML FirstGlance]. <br>
	~~Description: Human aldolase A I98C~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xml FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xml OCA], [https://pdbe.org/6xml PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xml RCSB], [https://www.ebi.ac.uk/pdbsum/6xml PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xml ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Lamb, A.L~~]]		__TOC__
	[[Category: ~~Meneely, K.M~~]]		</StructureSection>
	[[Category: ~~Brewer, K~~]]		[[Category: Large Structures]]
			[[Category: Brewer K]]
			[[Category: Lamb AL]]
			[[Category: Meneely KM]]

OCA at 04:31, 19 August 2020

2020-08-19T04:31:08Z

← Older revision		Revision as of 04:31, 19 August 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 6xml is ON HOLD		The entry 6xml is ON HOLD until Paper Publication

	Authors: Meneely, K.M., Brewer, K., Lamb, A.L.		Authors: Meneely, K.M., Brewer, K., Lamb, A.L.

OCA at 06:43, 22 July 2020

2020-07-22T06:43:29Z

← Older revision		Revision as of 06:43, 22 July 2020
Line 5:		Line 5:
	Authors: Meneely, K.M., Brewer, K., Lamb, A.L.		Authors: Meneely, K.M., Brewer, K., Lamb, A.L.

	Description: Human aldolase A ~~wild type~~		Description: Human aldolase A I98C
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Brewer, K]]~~
	[[Category: Lamb, A.L]]		[[Category: Lamb, A.L]]
	[[Category: Meneely, K.M]]		[[Category: Meneely, K.M]]
			[[Category: Brewer, K]]

OCA: Protected "6xml" [edit=sysop:move=sysop]

2020-07-08T10:59:17Z

Protected "6xml" [edit=sysop:move=sysop]

← Older revision	Revision as of 10:59, 8 July 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 6xml is ON HOLD Authors: Meneely, K.M., Brewer, K., Lamb, A.L. Description: Human aldolase A wild type Category: Unreleased Structures [[Catego...

2020-07-08T10:59:16Z

New page: '''Unreleased structure''' The entry 6xml is ON HOLD Authors: Meneely, K.M., Brewer, K., Lamb, A.L. Description: Human aldolase A wild type Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 6xml is ON HOLD

Authors: Meneely, K.M., Brewer, K., Lamb, A.L.

Description: Human aldolase A wild type
[[Category: Unreleased Structures]]
[[Category: Brewer, K]]
[[Category: Lamb, A.L]]
[[Category: Meneely, K.M]]