7ask - Revision history

OCA at 07:45, 1 May 2024

2024-05-01T07:45:46Z

← Older revision		Revision as of 07:45, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ask]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ask]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.3Å</td></tr>
	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height~~: ~~3em;~~'>~~[[7ase\|7ase]]~~</~~div~~></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style~~=~~"background-color:#fffaf0;">~~		== Function ==
	=~~= Publication Abstract from PubMed~~ ==		[https://www.uniprot.org/uniprot/Q4E5Z1_TRYCC Q4E5Z1_TRYCC]
	Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNAi(Met) and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9(S), 7(S), and 6(S), and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. ~~cruzi and mass spectrometry data~~.

	Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.,Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Valasek LS, Hashem Y Cell Rep. 2020 Dec 22;33(12):108534. doi: 10.1016/j.celrep.2020.108534. PMID:33357443<ref>PMID:33357443</ref>		==See Also==
			*[[Helicase 3D structures\|Helicase 3D structures]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7ask" style="background-color:#fffaf0;"></div>~~
	== ~~References~~ ==
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Trypanosoma cruzi]]		[[Category: Trypanosoma cruzi]]
	[[Category: Bochler, A]]		[[Category: Bochler A]]
	[[Category: Cistia~~, M L.Del~~]]		[[Category: Brito Querido J]]
	[[Category: Hashem, Y]]		[[Category: Del Cistia ML]]
	[[Category: Kuhn, L]]		[[Category: Hashem Y]]
	[[Category: Prilepskaja, T]]		[[Category: Kuhn L]]
	[[Category: ~~Querido, J Brito]]~~		[[Category: Prilepskaja T]]
	~~[[Category:~~ Ribeiro, A ~~Rimoldi~~]]		[[Category: Rimoldi Ribeiro A]]
	[[Category: Soufari, H]]		[[Category: Soufari H]]
	[[Category: Valasek~~, L S]]~~		[[Category: Valasek LS]]
	~~[[Category: Factor]]~~
	~~[[Category: Helicase]]~~
	~~[[Category: Kinetoplastid]]~~
	~~[[Category: Preinitiation]]~~
	~~[[Category: Translation~~]]

OCA at 08:30, 21 April 2021

2021-04-21T08:30:44Z

← Older revision		Revision as of 08:30, 21 April 2021
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	==43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP==		==43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP==
	<StructureSection load='7ask' size='340' side='right'caption='[[7ask]]' scene=''>		<StructureSection load='7ask' size='340' side='right'caption='[[7ask]], [[Resolution\|resolution]] 4.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ask]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_cruzi Trypanosoma cruzi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
			<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7ase\|7ase]]</div></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Canonical mRNA translation in eukaryotes begins with the formation of the 43S pre-initiation complex (PIC). Its assembly requires binding of initiator Met-tRNAi(Met) and several eukaryotic initiation factors (eIFs) to the small ribosomal subunit (40S). Compared to their mammalian hosts, trypanosomatids present significant structural differences in their 40S, suggesting substantial variability in translation initiation. Here, we determine the structure of the 43S PIC from Trypanosoma cruzi, the parasite causing Chagas disease. Our structure shows numerous specific features, such as the variant eIF3 structure and its unique interactions with the large rRNA expansion segments (ESs) 9(S), 7(S), and 6(S), and the association of a kinetoplastid-specific DDX60-like helicase. It also reveals the 40S-binding site of the eIF5 C-terminal domain and structures of key terminal tails of several conserved eIFs underlying their activities within the PIC. Our results are corroborated by glutathione S-transferase (GST) pull-down assays in both human and T. cruzi and mass spectrometry data.

			Structural Differences in Translation Initiation between Pathogenic Trypanosomatids and Their Mammalian Hosts.,Bochler A, Querido JB, Prilepskaja T, Soufari H, Simonetti A, Del Cistia ML, Kuhn L, Ribeiro AR, Valasek LS, Hashem Y Cell Rep. 2020 Dec 22;33(12):108534. doi: 10.1016/j.celrep.2020.108534. PMID:33357443<ref>PMID:33357443</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ask" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Bochler A~~]]		[[Category: Trypanosoma cruzi]]
	[[Category: ~~Brito Querido J~~]]		[[Category: Bochler, A]]
	[[Category: Del ~~Cistia ML~~]]		[[Category: Cistia, M L.Del]]
	[[Category: Hashem Y]]		[[Category: Hashem, Y]]
	[[Category: Kuhn L]]		[[Category: Kuhn, L]]
	[[Category: Prilepskaja T]]		[[Category: Prilepskaja, T]]
	[[Category: ~~Rimoldi~~ Ribeiro A]]		[[Category: Querido, J Brito]]
	[[Category: Soufari H]]		[[Category: Ribeiro, A Rimoldi]]
	[[Category: Valasek LS]]		[[Category: Soufari, H]]
			[[Category: Valasek, L S]]
			[[Category: Factor]]
			[[Category: Helicase]]
			[[Category: Kinetoplastid]]
			[[Category: Preinitiation]]
			[[Category: Translation]]

OCA at 06:45, 14 April 2021

2021-04-14T06:45:23Z

← Older revision		Revision as of 06:45, 14 April 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ask is ~~ON HOLD until Paper Publication~~		==43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP==
			<StructureSection load='7ask' size='340' side='right'caption='[[7ask]]' scene=''>
	~~Authors~~: ~~Bochler~~, A.~~, Brito Querido, J~~.~~, Prilepskaja, T~~., ~~Soufari, H~~., ~~Del Cistia, M~~.L.~~, Kuhn, L~~., ~~Rimoldi Ribeiro, A~~.~~, Valasek, L~~.S., ~~Hashem, Y~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ASK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ASK FirstGlance]. <br>
	~~Description: 43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ask FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ask OCA], [https://pdbe.org/7ask PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ask RCSB], [https://www.ebi.ac.uk/pdbsum/7ask PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ask ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Soufari, H~~]]		__TOC__
	[[Category: Brito Querido, J]]		</StructureSection>
	[[Category: ~~Kuhn, L~~]]		[[Category: Large Structures]]
	[[Category: Hashem, Y]]		[[Category: Bochler A]]
	[[Category: ~~Bochler, A~~]]		[[Category: Brito Querido J]]
	[[Category: ~~Rimoldi Ribeiro, A~~]]		[[Category: Del Cistia ML]]
	[[Category: ~~Valasek, L.S~~]]		[[Category: Hashem Y]]
	[[Category: ~~Del Cistia, M.L~~]]		[[Category: Kuhn L]]
	[[Category: ~~Prilepskaja, T~~]]		[[Category: Prilepskaja T]]
			[[Category: Rimoldi Ribeiro A]]
			[[Category: Soufari H]]
			[[Category: Valasek LS]]

OCA at 05:28, 2 December 2020

2020-12-02T05:28:37Z

← Older revision		Revision as of 05:28, 2 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ask is ON HOLD		The entry 7ask is ON HOLD until Paper Publication

	Authors: Bochler, A., Brito Querido, J., Prilepskaja, T., Soufari, H., Del Cistia, M.L., Kuhn, L., Rimoldi Ribeiro, A., Valasek, L.S., Hashem, Y.		Authors: Bochler, A., Brito Querido, J., Prilepskaja, T., Soufari, H., Del Cistia, M.L., Kuhn, L., Rimoldi Ribeiro, A., Valasek, L.S., Hashem, Y.
Line 9:		Line 9:
	[[Category: Soufari, H]]		[[Category: Soufari, H]]
	[[Category: Brito Querido, J]]		[[Category: Brito Querido, J]]
	~~[[Category: Del Cistia, M.L]]~~
	[[Category: Kuhn, L]]		[[Category: Kuhn, L]]
			[[Category: Hashem, Y]]
	[[Category: Bochler, A]]		[[Category: Bochler, A]]
	[[Category: Rimoldi Ribeiro, A]]		[[Category: Rimoldi Ribeiro, A]]
			[[Category: Valasek, L.S]]
			[[Category: Del Cistia, M.L]]
	[[Category: Prilepskaja, T]]		[[Category: Prilepskaja, T]]
	~~[[Category: Hashem, Y]]~~
	~~[[Category: Valasek, L.S]]~~

OCA: Protected "7ask" [edit=sysop:move=sysop]

2020-11-04T05:24:03Z

Protected "7ask" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:24, 4 November 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ask is ON HOLD Authors: Bochler, A., Brito Querido, J., Prilepskaja, T., Soufari, H., Del Cistia, M.L., Kuhn, L., Rimoldi Ribeiro, A., Valasek, L.S...

2020-11-04T05:24:02Z

New page: '''Unreleased structure''' The entry 7ask is ON HOLD Authors: Bochler, A., Brito Querido, J., Prilepskaja, T., Soufari, H., Del Cistia, M.L., Kuhn, L., Rimoldi Ribeiro, A., Valasek, L.S...

New page

'''Unreleased structure'''

The entry 7ask is ON HOLD

Authors: Bochler, A., Brito Querido, J., Prilepskaja, T., Soufari, H., Del Cistia, M.L., Kuhn, L., Rimoldi Ribeiro, A., Valasek, L.S., Hashem, Y.

Description: 43S preinitiation complex from Trypanosoma cruzi with the kDDX60 helicase bound with ATP
[[Category: Unreleased Structures]]
[[Category: Soufari, H]]
[[Category: Brito Querido, J]]
[[Category: Del Cistia, M.L]]
[[Category: Kuhn, L]]
[[Category: Bochler, A]]
[[Category: Rimoldi Ribeiro, A]]
[[Category: Prilepskaja, T]]
[[Category: Hashem, Y]]
[[Category: Valasek, L.S]]