7awk - Revision history

OCA at 06:11, 19 June 2024

2024-06-19T06:11:39Z

← Older revision		Revision as of 06:11, 19 June 2024
Line 3:		Line 3:
	<StructureSection load='7awk' size='340' side='right'caption='[[7awk]], [[Resolution\|resolution]] 1.91Å' scene=''>		<StructureSection load='7awk' size='340' side='right'caption='[[7awk]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7awk]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.91Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	[[https://www.uniprot.org/uniprot/HIGB1_MYCTU HIGB1_MYCTU]] Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth (PubMed:20011113, PubMed:23927792). Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endoribonuclease; also degrades E.coli and M-smegmatis tmRNA (PubMed:23927792). Its toxic effect is neutralized by coexpression with antitoxin HigA. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system (PubMed:21536872).<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:21536872</ref> <ref>PMID:23927792</ref>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	Toxins of toxin-antitoxin systems use diverse mechanisms to control bacterial growth. Here, we focus on the deleterious toxin of the atypical tripartite toxin-antitoxin-chaperone (TAC) system of Mycobacterium tuberculosis, whose inhibition requires the concerted action of the antitoxin and its dedicated SecB-like chaperone. We show that the TAC toxin is a bona fide ribonuclease and identify exact cleavage sites in mRNA targets on a transcriptome-wide scale in vivo. mRNA cleavage by the toxin occurs after the second nucleotide of the ribosomal A-site codon during translation, with a strong preference for CCA codons in vivo. Finally, we report the cryo-EM structure of the ribosome-bound TAC toxin in the presence of native M. tuberculosis cspA mRNA, revealing the specific mechanism by which the TAC toxin interacts with the ribosome and the tRNA in the P-site to cleave its mRNA target.

	Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis.,Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D'urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P Nat Commun. 2022 May 12;13(1):2641. doi: 10.1038/s41467-022-30373-w. PMID:35552387<ref>PMID:35552387</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7awk" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bigot~~, D J~~]]		[[Category: Bigot DJ]]
	[[Category: Guillet, V]]		[[Category: Guillet V]]
	[[Category: Mourey, L]]		[[Category: Mourey L]]
	~~[[Category: Mycobacterium tuberculosis]]~~
	~~[[Category: Rnase]]~~
	~~[[Category: Toxin]]~~
	~~[[Category: Toxin-antitoxin-chaperone~~]]

OCA at 08:10, 25 May 2022

2022-05-25T08:10:33Z

← Older revision		Revision as of 08:10, 25 May 2022
Line 8:		Line 8:
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/HIGB1_MYCTU HIGB1_MYCTU]] Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth (PubMed:20011113, PubMed:23927792). Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endoribonuclease; also degrades E.coli and M-smegmatis tmRNA (PubMed:23927792). Its toxic effect is neutralized by coexpression with antitoxin HigA. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system (PubMed:21536872).<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:21536872</ref> <ref>PMID:23927792</ref>		[[https://www.uniprot.org/uniprot/HIGB1_MYCTU HIGB1_MYCTU]] Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth (PubMed:20011113, PubMed:23927792). Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endoribonuclease; also degrades E.coli and M-smegmatis tmRNA (PubMed:23927792). Its toxic effect is neutralized by coexpression with antitoxin HigA. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system (PubMed:21536872).<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:21536872</ref> <ref>PMID:23927792</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Toxins of toxin-antitoxin systems use diverse mechanisms to control bacterial growth. Here, we focus on the deleterious toxin of the atypical tripartite toxin-antitoxin-chaperone (TAC) system of Mycobacterium tuberculosis, whose inhibition requires the concerted action of the antitoxin and its dedicated SecB-like chaperone. We show that the TAC toxin is a bona fide ribonuclease and identify exact cleavage sites in mRNA targets on a transcriptome-wide scale in vivo. mRNA cleavage by the toxin occurs after the second nucleotide of the ribosomal A-site codon during translation, with a strong preference for CCA codons in vivo. Finally, we report the cryo-EM structure of the ribosome-bound TAC toxin in the presence of native M. tuberculosis cspA mRNA, revealing the specific mechanism by which the TAC toxin interacts with the ribosome and the tRNA in the P-site to cleave its mRNA target.

			Substrate recognition and cryo-EM structure of the ribosome-bound TAC toxin of Mycobacterium tuberculosis.,Mansour M, Giudice E, Xu X, Akarsu H, Bordes P, Guillet V, Bigot DJ, Slama N, D'urso G, Chat S, Redder P, Falquet L, Mourey L, Gillet R, Genevaux P Nat Commun. 2022 May 12;13(1):2641. doi: 10.1038/s41467-022-30373-w. PMID:35552387<ref>PMID:35552387</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7awk" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:20, 18 May 2022

2022-05-18T10:20:04Z

← Older revision		Revision as of 10:20, 18 May 2022
Line 1:		Line 1:

	==Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)==		==Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)==
	<StructureSection load='7awk' size='340' side='right'caption='[[7awk]]' scene=''>		<StructureSection load='7awk' size='340' side='right'caption='[[7awk]], [[Resolution\|resolution]] 1.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br>		<table><tr><td colspan='2'>[[7awk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/HIGB1_MYCTU HIGB1_MYCTU]] Toxic component of an atypical, type II toxin-antitoxin chaperone (TAC) system. Upon expression in M.smegmatis inhibits colony formation and cell growth (PubMed:20011113, PubMed:23927792). Ectopic expression in wild-type M.tuberculosis has no effect on cell growth; ectopic expression in a triple higB1-higA1-Rv1957 (delta TAC) disruption mutant causes growth arrest, killing a considerable proportion of the cells. Increased ectopic expression leads to decreased levels of IdeR- and Zur-regulated genes as well as cleavage within the mRNA region of tmRNA (transfer-mRNA), strongly suggesting it is an endoribonuclease; also degrades E.coli and M-smegmatis tmRNA (PubMed:23927792). Its toxic effect is neutralized by coexpression with antitoxin HigA. Neutralization of HigB1 toxin in E.coli or M.marinum also requires SecB-like chaperone Rv1957, making this the first toxin-antitoxin chaperone (TAC) system (PubMed:21536872).<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> <ref>PMID:21536872</ref> <ref>PMID:23927792</ref>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bigot DJ]]		[[Category: Bigot, D J]]
	[[Category: Guillet V]]		[[Category: Guillet, V]]
	[[Category: Mourey L]]		[[Category: Mourey, L]]
			[[Category: Mycobacterium tuberculosis]]
			[[Category: Rnase]]
			[[Category: Toxin]]
			[[Category: Toxin-antitoxin-chaperone]]

OCA at 12:32, 24 November 2021

2021-11-24T12:32:55Z

← Older revision		Revision as of 12:32, 24 November 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7awk is ~~ON HOLD until Paper Publication~~		==Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)==
			<StructureSection load='7awk' size='340' side='right'caption='[[7awk]]' scene=''>
	~~Authors~~: ~~Bigot~~, D.J., ~~Guillet~~, V., ~~Mourey~~, L.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AWK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AWK FirstGlance]. <br>
	~~Description: Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7awk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7awk OCA], [https://pdbe.org/7awk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7awk RCSB], [https://www.ebi.ac.uk/pdbsum/7awk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7awk ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Mourey, L~~]]		__TOC__
	[[Category: Guillet, V]]		</StructureSection>
	[[Category: ~~Bigot, D.J~~]]		[[Category: Large Structures]]
			[[Category: Bigot DJ]]
			[[Category: Guillet V]]
			[[Category: Mourey L]]

OCA: Protected "7awk" [edit=sysop:move=sysop]

2020-11-18T07:54:01Z

Protected "7awk" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:54, 18 November 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7awk is ON HOLD until Paper Publication Authors: Bigot, D.J., Guillet, V., Mourey, L. Description: Crystal structure of the HigB1 toxin mutant K95A...

2020-11-18T07:54:00Z

New page: '''Unreleased structure''' The entry 7awk is ON HOLD until Paper Publication Authors: Bigot, D.J., Guillet, V., Mourey, L. Description: Crystal structure of the HigB1 toxin mutant K95A...

New page

'''Unreleased structure'''

The entry 7awk is ON HOLD until Paper Publication

Authors: Bigot, D.J., Guillet, V., Mourey, L.

Description: Crystal structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955)
[[Category: Unreleased Structures]]
[[Category: Mourey, L]]
[[Category: Guillet, V]]
[[Category: Bigot, D.J]]