OCA at 14:10, 17 November 2021

2021-11-17T14:10:13Z

← Older revision		Revision as of 14:10, 17 November 2021
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	==A de novo pentameric coiled-coil assembly: CC-Type2-(TgLaId)4-W19BrPhe.==		==A de novo pentameric coiled-coil assembly: CC-Type2-(TgLaId)4-W19BrPhe.==
	<StructureSection load='7bas' size='340' side='right'caption='[[7bas]]' scene=''>		<StructureSection load='7bas' size='340' side='right'caption='[[7bas]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BAS FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bas]] is a 5 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BAS FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bas OCA], [https://pdbe.org/7bas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bas RCSB], [https://www.ebi.ac.uk/pdbsum/7bas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bas ProSAT]</span></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=4BF:4-BROMO-L-PHENYLALANINE'>4BF</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bas OCA], [https://pdbe.org/7bas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bas RCSB], [https://www.ebi.ac.uk/pdbsum/7bas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bas ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The rational design of linear peptides that assemble controllably and predictably in water is challenging. Short sequences must encode unique target structures and avoid alternative states. However, the non-covalent forces that stabilize and discriminate between states are weak. Nonetheless, for alpha-helical coiled-coil assemblies considerable progress has been made in rational de novo design. In these, sequence repeats of nominally hydrophobic (h) and polar (p) residues, hpphppp, direct the assembly of amphipathic helices into dimeric to tetrameric bundles. Expanding this pattern to hpphhph can produce larger alpha-helical barrels. Here, we show that pentameric to nonameric barrels are accessed by varying the residue at one of the h sites. In peptides with four L/I-K-E-I-A-x-Z repeats, decreasing the size of Z from threonine to serine to alanine to glycine gives progressively larger oligomers. X-ray crystal structures of the resulting alpha-helical barrels rationalize this: side chains at Z point directly into the helical interfaces, and smaller residues allow closer helix contacts and larger assemblies.

			Coiled coils 9-to-5: rational de novo design of alpha-helical barrels with tunable oligomeric states.,Dawson WM, Martin FJO, Rhys GG, Shelley KL, Brady RL, Woolfson DN Chem Sci. 2021 Apr 13;12(20):6923-6928. doi: 10.1039/d1sc00460c. eCollection 2021, May 26. PMID:34745518<ref>PMID:34745518</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7bas" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Brady RL]]		[[Category: Brady, R L]]
	[[Category: Dawson WM]]		[[Category: Dawson, W M]]
	[[Category: Martin ~~FJO~~]]		[[Category: Martin, F J.O]]
	[[Category: Shelley K]]		[[Category: Shelley, K]]
	[[Category: Woolfson DN]]		[[Category: Woolfson, D N]]
			[[Category: Alpha]]
			[[Category: Barrel]]
			[[Category: De novo protein]]
			[[Category: Helical]]
			[[Category: Pentamer]]

OCA at 05:19, 28 April 2021

2021-04-28T05:19:23Z

← Older revision		Revision as of 05:19, 28 April 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7bas is ~~ON HOLD until Paper Publication~~		==A de novo pentameric coiled-coil assembly: CC-Type2-(TgLaId)4-W19BrPhe.==
			<StructureSection load='7bas' size='340' side='right'caption='[[7bas]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BAS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BAS FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bas OCA], [https://pdbe.org/7bas PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bas RCSB], [https://www.ebi.ac.uk/pdbsum/7bas PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bas ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Brady RL]]
			[[Category: Dawson WM]]
			[[Category: Martin FJO]]
			[[Category: Shelley K]]
			[[Category: Woolfson DN]]

OCA at 06:34, 20 January 2021

2021-01-20T06:34:51Z

← Older revision		Revision as of 06:34, 20 January 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7bas is ON HOLD		The entry 7bas is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7bas" [edit=sysop:move=sysop]

2020-12-24T07:47:29Z

Protected "7bas" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:47, 24 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7bas is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-24T07:47:27Z

New page: '''Unreleased structure''' The entry 7bas is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bas is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7bas - Revision history

OCA at 14:10, 17 November 2021

OCA at 05:19, 28 April 2021

OCA at 06:34, 20 January 2021

OCA: Protected "7bas" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7bas is ON HOLD Authors: Description: Category: Unreleased Structures