7bb5 - Revision history

OCA at 07:47, 1 May 2024

2024-05-01T07:47:53Z

← Older revision		Revision as of 07:47, 1 May 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC]		[https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC]
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	CRISPR-Cas systems are prokaryotic adaptive immune systems that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes and phages gave rise to phage anti-CRISPR (Acr) proteins that act as a counter defence against CRISPR-Cas systems by inhibiting the effector complex. Here, we used a combination of bulk biochemical experiments, X-ray crystallography and single-molecule techniques to explore the inhibitory activity of AcrIF6 and AcrIF9 proteins against the type I-F CRISPR-Cas system from Aggregatibacter actinomycetemcomitans (Aa). We showed that AcrIF6 and AcrIF9 proteins hinder Aa-Cascade complex binding to target DNA. We solved a crystal structure of Aa1-AcrIF9 protein, which differ from other known AcrIF9 proteins by an additional structurally important loop presumably involved in the interaction with Cascade. We revealed that AcrIF9 association with Aa-Cascade promotes its binding to off-target DNA sites, which facilitates inhibition of CRISPR-Cas protection.

	Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9.,Kupcinskaite E, Tutkus M, Kopustas A, Asmontas S, Jankunec M, Zaremba M, Tamulaitiene G, Sinkunas T Sci Rep. 2022 Sep 15;12(1):15548. doi: 10.1038/s41598-022-19797-y. PMID:36109551<ref>PMID:36109551</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7bb5" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:28, 7 December 2022

2022-12-07T08:28:10Z

← Older revision		Revision as of 08:28, 7 December 2022
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	==Crystal structure of anti-CRISPR protein AcrIF9==		==Crystal structure of anti-CRISPR protein AcrIF9==
	<StructureSection load='7bb5' size='340' side='right'caption='[[7bb5]]' scene=''>		<StructureSection load='7bb5' size='340' side='right'caption='[[7bb5]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bb5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aggregatibacter_actinomycetemcomitans_serotype_e_str._SC1083 Aggregatibacter actinomycetemcomitans serotype e str. SC1083]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/G4A6K5_AGGAC G4A6K5_AGGAC]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			CRISPR-Cas systems are prokaryotic adaptive immune systems that protect against phages and other invading nucleic acids. The evolutionary arms race between prokaryotes and phages gave rise to phage anti-CRISPR (Acr) proteins that act as a counter defence against CRISPR-Cas systems by inhibiting the effector complex. Here, we used a combination of bulk biochemical experiments, X-ray crystallography and single-molecule techniques to explore the inhibitory activity of AcrIF6 and AcrIF9 proteins against the type I-F CRISPR-Cas system from Aggregatibacter actinomycetemcomitans (Aa). We showed that AcrIF6 and AcrIF9 proteins hinder Aa-Cascade complex binding to target DNA. We solved a crystal structure of Aa1-AcrIF9 protein, which differ from other known AcrIF9 proteins by an additional structurally important loop presumably involved in the interaction with Cascade. We revealed that AcrIF9 association with Aa-Cascade promotes its binding to off-target DNA sites, which facilitates inhibition of CRISPR-Cas protection.

			Disarming of type I-F CRISPR-Cas surveillance complex by anti-CRISPR proteins AcrIF6 and AcrIF9.,Kupcinskaite E, Tutkus M, Kopustas A, Asmontas S, Jankunec M, Zaremba M, Tamulaitiene G, Sinkunas T Sci Rep. 2022 Sep 15;12(1):15548. doi: 10.1038/s41598-022-19797-y. PMID:36109551<ref>PMID:36109551</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7bb5" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aggregatibacter actinomycetemcomitans serotype e str. SC1083]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kupcinskaite E]]		[[Category: Kupcinskaite E]]
	[[Category: Sinkunas T]]		[[Category: Sinkunas T]]
	[[Category: Tamulaitiene G]]		[[Category: Tamulaitiene G]]

OCA at 12:33, 24 November 2021

2021-11-24T12:33:20Z

← Older revision		Revision as of 12:33, 24 November 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7bb5 is ~~ON HOLD until Paper Publication~~		==Crystal structure of anti-CRISPR protein AcrIF9==
			<StructureSection load='7bb5' size='340' side='right'caption='[[7bb5]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BB5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BB5 FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bb5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bb5 OCA], [https://pdbe.org/7bb5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bb5 RCSB], [https://www.ebi.ac.uk/pdbsum/7bb5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bb5 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Kupcinskaite E]]
			[[Category: Sinkunas T]]
			[[Category: Tamulaitiene G]]

OCA at 05:14, 30 December 2020

2020-12-30T05:14:33Z

← Older revision		Revision as of 05:14, 30 December 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7bb5 is ON HOLD		The entry 7bb5 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7bb5" [edit=sysop:move=sysop]

2020-12-24T07:48:46Z

Protected "7bb5" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:48, 24 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7bb5 is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-24T07:48:45Z

New page: '''Unreleased structure''' The entry 7bb5 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bb5 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]