OCA at 08:21, 4 March 2026

2026-03-04T08:21:20Z

← Older revision		Revision as of 08:21, 4 March 2026
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	====		==Crystal structure of ubiquitin charged Ube2N (Ube2N~Ub) in complex with Ube2V2==
	<StructureSection load='7bbf' size='340' side='right'caption='[[7bbf]]' scene=''>		<StructureSection load='7bbf' size='340' side='right'caption='[[7bbf]], [[Resolution\|resolution]] 2.54Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bbf]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbf OCA], [https://pdbe.org/7bbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbf RCSB], [https://www.ebi.ac.uk/pdbsum/7bbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.54Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbf OCA], [https://pdbe.org/7bbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbf RCSB], [https://www.ebi.ac.uk/pdbsum/7bbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/UB2V2_HUMAN UB2V2_HUMAN] Has no ubiquitin ligase activity on its own. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.<ref>PMID:9705497</ref> <ref>PMID:10089880</ref> <ref>PMID:14562038</ref> <ref>PMID:20061386</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Attachment of ubiquitin (Ub) to proteins is one of the most abundant and versatile of all posttranslational modifications and affects outcomes in essentially all physiological processes. RING E3 ligases target E2 Ub-conjugating enzymes to the substrate, resulting in its ubiquitination. However, the mechanism by which a ubiquitin chain is formed on the substrate remains elusive. Here we demonstrate how substrate binding can induce a specific RING topology that enables self-ubiquitination. By analyzing a catalytically trapped structure showing the initiation of TRIM21 RING-anchored ubiquitin chain elongation, and in combination with a kinetic study, we illuminate the chemical mechanism of ubiquitin conjugation. Moreover, biochemical and cellular experiments show that the topology found in the structure can be induced by substrate binding. Our results provide insights into ubiquitin chain formation on a structural, biochemical and cellular level with broad implications for targeted protein degradation.

			RING domains act as both substrate and enzyme in a catalytic arrangement to drive self-anchored ubiquitination.,Kiss L, Clift D, Renner N, Neuhaus D, James LC Nat Commun. 2021 Feb 22;12(1):1220. doi: 10.1038/s41467-021-21443-6. PMID:33619271<ref>PMID:33619271</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7bbf" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[3D structures of ubiquitin\|3D structures of ubiquitin]]
			*[[3D structures of ubiquitin conjugating enzyme\|3D structures of ubiquitin conjugating enzyme]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: James LC]]
			[[Category: Kiss L]]
			[[Category: Neuhaus D]]

OCA at 07:56, 27 January 2021

2021-01-27T07:56:25Z

← Older revision		Revision as of 07:56, 27 January 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7bbf is ~~ON HOLD until sometime in~~ the ~~future~~		====
			<StructureSection load='7bbf' size='340' side='right'caption='[[7bbf]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bbf OCA], [https://pdbe.org/7bbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bbf RCSB], [https://www.ebi.ac.uk/pdbsum/7bbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bbf ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA: Protected "7bbf" [edit=sysop:move=sysop]

2020-12-24T07:49:52Z

Protected "7bbf" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:49, 24 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7bbf is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

2020-12-24T07:49:50Z

New page: '''Unreleased structure''' The entry 7bbf is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7bbf is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]

7bbf - Revision history

OCA at 08:21, 4 March 2026

OCA at 07:56, 27 January 2021

OCA: Protected "7bbf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7bbf is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures