7bee - Revision history

OCA at 09:21, 9 October 2024

2024-10-09T09:21:10Z

@@ Line 8: / Line 8: @@
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bee OCA], [https://pdbe.org/7bee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bee RCSB], [https://www.ebi.ac.uk/pdbsum/7bee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bee ProSAT]</span></td></tr>
 </table>
 __TOC__
 </StructureSection>

OCA at 12:26, 1 February 2024

2024-02-01T12:26:50Z

← Older revision		Revision as of 12:26, 1 February 2024
Line 1:		Line 1:

	====		==Crystal structure of a Hsp47-collagen peptide complex==
	<StructureSection load='7bee' size='340' side='right'caption='[[7bee]]' scene=''>		<StructureSection load='7bee' size='340' side='right'caption='[[7bee]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bee]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BEE FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bee OCA], [https://pdbe.org/7bee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bee RCSB], [https://www.ebi.ac.uk/pdbsum/7bee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bee ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.939Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bee OCA], [https://pdbe.org/7bee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bee RCSB], [https://www.ebi.ac.uk/pdbsum/7bee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bee ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A8I3N7V2_CANLF A0A8I3N7V2_CANLF] Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.[ARBA:ARBA00025405]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Collagens play important roles in development and homeostasis in most higher organisms. In order to function, collagens require the specific chaperone HSP47 for proper folding and secretion. HSP47 is known to bind to the collagen triple helix, but the exact positions and numbers of binding sites are not clear. Here, we employed a collagen II peptide library to characterize high-affinity binding sites for HSP47. We show that many previously predicted binding sites have very low affinities due to the presence of a negatively charged amino acid in the binding motif. In contrast, large hydrophobic amino acids such as phenylalanine at certain positions in the collagen sequence increase binding strength. For further characterization, we determined two crystal structures of HSP47 bound to peptides containing phenylalanine or leucine. These structures deviate significantly from previously published ones in which different collagen sequences were used. They reveal local conformational rearrangements of HSP47 at the binding site to accommodate the large hydrophobic side chain from the middle strand of the collagen triple helix and, most surprisingly, possess an altered binding stoichiometry in the form of a 1:1 complex. This altered stoichiometry is explained by steric collisions with the second HSP47 molecule present in all structures determined thus far caused by the newly introduced large hydrophobic residue placed on the trailing strand. This exemplifies the importance of considering all three sites of homotrimeric collagen as independent interaction surfaces and may provide insight into the formation of higher oligomeric complexes at promiscuous collagen-binding sites.

			Collagen's primary structure determines collagen:HSP47 complex stoichiometry.,Abraham ET, Oecal S, Morgelin M, Schmid PWN, Buchner J, Baumann U, Gebauer JM J Biol Chem. 2021 Dec;297(6):101169. doi: 10.1016/j.jbc.2021.101169. Epub 2021 , Sep 4. PMID:34487762<ref>PMID:34487762</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7bee" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Canis lupus familiaris]]
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Abraham ET]]
			[[Category: Baumann U]]
			[[Category: Gebauer JM]]

OCA at 06:13, 10 November 2021

2021-11-10T06:13:51Z

← Older revision		Revision as of 06:13, 10 November 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7bee ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7bee' size='340' side='right'caption='[[7bee]]' scene=''>
	~~Authors~~: ~~Abraham, E~~.T.~~, Gebauer, J~~.M.~~, Baumann, U~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Crystal structure of a Hsp47-collagen peptide complex~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bee OCA], [https://pdbe.org/7bee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bee RCSB], [https://www.ebi.ac.uk/pdbsum/7bee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bee ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[~~Category~~: ~~Baumann~~, U]]		__TOC__
	[[Category: ~~Gebauer, J.M~~]]		</StructureSection>
	[[Category: ~~Abraham, E.T~~]]		[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 13:06, 3 March 2021

2021-03-03T13:06:56Z

← Older revision		Revision as of 13:06, 3 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7bee is ON HOLD		The entry 7bee is ON HOLD until Paper Publication

	Authors: Abraham, E.T., Gebauer, J.M., Baumann, U.		Authors: Abraham, E.T., Gebauer, J.M., Baumann, U.

OCA: Protected "7bee" [edit=sysop:move=sysop]

2020-12-30T05:19:28Z

Protected "7bee" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:19, 30 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7bee is ON HOLD Authors: Abraham, E.T., Gebauer, J.M., Baumann, U. Description: Crystal structure of a Hsp47-collagen peptide complex [[Category: U...

2020-12-30T05:19:27Z

New page: '''Unreleased structure''' The entry 7bee is ON HOLD Authors: Abraham, E.T., Gebauer, J.M., Baumann, U. Description: Crystal structure of a Hsp47-collagen peptide complex [[Category: U...

New page

'''Unreleased structure'''

The entry 7bee is ON HOLD

Authors: Abraham, E.T., Gebauer, J.M., Baumann, U.

Description: Crystal structure of a Hsp47-collagen peptide complex
[[Category: Unreleased Structures]]
[[Category: Baumann, U]]
[[Category: Gebauer, J.M]]
[[Category: Abraham, E.T]]