7bpc - Revision history

OCA at 15:23, 29 November 2023

2023-11-29T15:23:42Z

← Older revision		Revision as of 15:23, 29 November 2023
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	==Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with 2,5-DHBA==		==Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with 2,5-DHBA==
	<StructureSection load='7bpc' size='340' side='right'caption='[[7bpc]]' scene=''>		<StructureSection load='7bpc' size='340' side='right'caption='[[7bpc]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BPC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7BPC FirstGlance]. <br>		<table><tr><td colspan='2'>[[7bpc]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Fusarium_oxysporum Fusarium oxysporum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BPC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BPC FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7bpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpc OCA], [~~http~~://pdbe.org/7bpc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7bpc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7bpc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpc ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTQ:2,5-DIHYDROXYBENZOIC+ACID'>GTQ</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpc OCA], [https://pdbe.org/7bpc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bpc RCSB], [https://www.ebi.ac.uk/pdbsum/7bpc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpc ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/N1S495_FUSC4 N1S495_FUSC4]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			A 2,3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum (2,3-DHBD_Fo) has a relatively high catalytic efficiency for the decarboxylation of 2,3-dihydroxybenzoic acid (DHBA) and carboxylation of catechol, thus it has a different substrate spectrum from other benzoic acid decarboxylases. We have determined the structures of 2,3-DHBD_Fo in its apo form and complexes with catechol or 2,5-dihydroxybenzoic acid at 1.55, 1.97, and 2.45 A resolution, respectively. The crystal structures of 2,3-DHBD_Fo show that the enzyme exists as a homotetramer, and each active center has a Zn(2+) ion coordinated by E8, H167, D291 and three water molecules. This is different from 2,6-DHBD from Rhizobium sporomusa, in which the Zn(2+) ion is also coordinated with H10. Surprisingly, mutation of A10 of 2,3-DHBD_Fo to His resulted in almost complete loss of the enzyme activity. Enzyme-substrate docking and site-directed mutation studies indicate that residue R233(Delta) interacts with the 3-hydroxy group of 2,3-DHBA, and plays an important role in substrate recognition for this enzyme, thus revealing the molecular basis 2,3-dihydroxybenzoic acid decarboxylase.

			2,3-Dihydroxybenzoic Acid Decarboxylase from Fusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism.,Song M, Zhang X, Liu W, Feng J, Cui Y, Yao P, Wang M, Guo RT, Wu Q, Zhu D Chembiochem. 2020 Oct 15;21(20):2950-2956. doi: 10.1002/cbic.202000244. Epub 2020, Jul 2. PMID:32421914<ref>PMID:32421914</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7bpc" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Fusarium oxysporum]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Feng JH]]		[[Category: Feng JH]]

OCA at 20:55, 28 October 2020

2020-10-28T20:55:00Z

← Older revision		Revision as of 20:55, 28 October 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7bpc is ~~ON HOLD until Mar 22 2022~~		==Crystal structure of 2, 3-dihydroxybenzoic acid decarboxylase from Fusarium oxysporum in complex with 2,5-DHBA==
			<StructureSection load='7bpc' size='340' side='right'caption='[[7bpc]]' scene=''>
	~~Authors~~: ~~Song, M~~.K., ~~Feng, J~~.H., ~~Liu~~, W.D., ~~Wu, Q~~.Q., ~~Zhu, D~~.M.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BPC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BPC FirstGlance]. <br>
	~~Description: Crystal structure of a decarboxylase from Fusarium oxysporum in complex with GTQ-1~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7bpc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bpc OCA], [http://pdbe.org/7bpc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7bpc RCSB], [http://www.ebi.ac.uk/pdbsum/7bpc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7bpc ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Zhu, D.M~~]]		__TOC__
	[[Category: ~~Wu, Q.Q~~]]		</StructureSection>
	[[Category: ~~Feng, J.H~~]]		[[Category: Large Structures]]
	[[Category: ~~Song, M.K~~]]		[[Category: Feng JH]]
	[[Category: ~~Liu, W.D~~]]		[[Category: Liu WD]]
			[[Category: Song MK]]
			[[Category: Wu QQ]]
			[[Category: Zhu DM]]

OCA at 04:34, 22 April 2020

2020-04-22T04:34:19Z

← Older revision		Revision as of 04:34, 22 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7bpc is ON HOLD		The entry 7bpc is ON HOLD until Mar 22 2022

	Authors: Song, M.K., Feng, J.H., Liu, W.D., Wu, Q.Q., Zhu, D.M.		Authors: Song, M.K., Feng, J.H., Liu, W.D., Wu, Q.Q., Zhu, D.M.
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	Description: Crystal structure of a decarboxylase from Fusarium oxysporum in complex with GTQ-1		Description: Crystal structure of a decarboxylase from Fusarium oxysporum in complex with GTQ-1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	~~[[Category: Song, M.K]]~~
	[[Category: Zhu, D.M]]		[[Category: Zhu, D.M]]
	~~[[Category: Liu, W.D]]~~
	[[Category: Wu, Q.Q]]		[[Category: Wu, Q.Q]]
	[[Category: Feng, J.H]]		[[Category: Feng, J.H]]
			[[Category: Song, M.K]]
			[[Category: Liu, W.D]]

OCA: Protected "7bpc" [edit=sysop:move=sysop]

2020-04-01T07:51:27Z

Protected "7bpc" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:51, 1 April 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7bpc is ON HOLD Authors: Song, M.K., Feng, J.H., Liu, W.D., Wu, Q.Q., Zhu, D.M. Description: Crystal structure of a decarboxylase from Fusarium oxy...

2020-04-01T07:51:24Z

New page: '''Unreleased structure''' The entry 7bpc is ON HOLD Authors: Song, M.K., Feng, J.H., Liu, W.D., Wu, Q.Q., Zhu, D.M. Description: Crystal structure of a decarboxylase from Fusarium oxy...

New page

'''Unreleased structure'''

The entry 7bpc is ON HOLD

Authors: Song, M.K., Feng, J.H., Liu, W.D., Wu, Q.Q., Zhu, D.M.

Description: Crystal structure of a decarboxylase from Fusarium oxysporum in complex with GTQ-1
[[Category: Unreleased Structures]]
[[Category: Song, M.K]]
[[Category: Zhu, D.M]]
[[Category: Liu, W.D]]
[[Category: Wu, Q.Q]]
[[Category: Feng, J.H]]