7c8a - Revision history

OCA at 15:58, 29 November 2023

2023-11-29T15:58:53Z

← Older revision		Revision as of 15:58, 29 November 2023
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	==Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-(bromoacetyl)naphthalene(Naph@ApPrx*)==		==Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-(bromoacetyl)naphthalene(Naph@ApPrx*)==
	<StructureSection load='7c8a' size='340' side='right'caption='[[7c8a]]' scene=''>		<StructureSection load='7c8a' size='340' side='right'caption='[[7c8a]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7C8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[7c8a]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeropyrum_pernix_K1 Aeropyrum pernix K1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7C8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8a OCA], [~~http~~://pdbe.org/7c8a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7c8a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7c8a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8a ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=FL3:1-naphthalen-2-ylethanone'>FL3</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8a OCA], [https://pdbe.org/7c8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7c8a RCSB], [https://www.ebi.ac.uk/pdbsum/7c8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8a ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/TDXH_AERPE TDXH_AERPE]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Direct control of the protein quaternary structure (QS) is challenging owing to the complexity of the protein structure. As a protein with a characteristic QS, peroxiredoxin from Aeropyrum pernix K1 (ApPrx) forms a decamer, wherein five dimers associate to form a ring. Here, we disrupted and reconstituted ApPrx QS via amino acid mutations and chemical modifications targeting hot spots for protein assembly. The decameric QS of an ApPrx* mutant, wherein all cysteine residues in wild-type ApPrx were mutated to serine, was destructed to dimers via an F80C mutation. The dimeric ApPrx*F80C mutant was then modified with a small molecule and successfully assembled as a decamer. Structural analysis confirmed that an artificially installed chemical moiety potentially facilitates suitable protein-protein interactions to rebuild a native structure. Rebuilding of dodecamer was also achieved through an additional amino acid mutation. This study describes a facile method to regulate the protein assembly state.

			Rebuilding Ring-Type Assembly of Peroxiredoxin by Chemical Modification.,Himiyama T, Tsuchiya Y, Yonezawa Y, Nakamura T Bioconjug Chem. 2020 Dec 17. doi: 10.1021/acs.bioconjchem.0c00587. PMID:33334100<ref>PMID:33334100</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7c8a" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Peroxiredoxin 3D structures\|Peroxiredoxin 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Aeropyrum pernix K1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Himiyama T]]		[[Category: Himiyama T]]
	[[Category: Nakamura T]]		[[Category: Nakamura T]]

OCA at 06:48, 30 December 2020

2020-12-30T06:48:27Z

← Older revision		Revision as of 06:48, 30 December 2020
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	~~'''Unreleased structure'''~~

	~~The entry 7c8a is ON HOLD until Paper Publication~~		==Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-(bromoacetyl)naphthalene(Naph@ApPrx*)==
			<StructureSection load='7c8a' size='340' side='right'caption='[[7c8a]]' scene=''>
	~~Authors: Himiyama, T., Nakamura, T.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7C8A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7C8A FirstGlance]. <br>
	~~Description:~~ Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-(bromoacetyl)naphthalene(Naph@ApPrx*)		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7c8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7c8a OCA], [http://pdbe.org/7c8a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7c8a RCSB], [http://www.ebi.ac.uk/pdbsum/7c8a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7c8a ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Himiyama, T]]		__TOC__
	[[Category: Nakamura, T]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Himiyama T]]
			[[Category: Nakamura T]]

OCA at 05:17, 10 June 2020

2020-06-10T05:17:46Z

← Older revision		Revision as of 05:17, 10 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7c8a is ON HOLD		The entry 7c8a is ON HOLD until Paper Publication

	Authors: Himiyama, T., Nakamura, T.		Authors: Himiyama, T., Nakamura, T.

OCA: Protected "7c8a" [edit=sysop:move=sysop]

2020-06-03T05:28:51Z

Protected "7c8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:28, 3 June 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7c8a is ON HOLD Authors: Himiyama, T., Nakamura, T. Description: Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modifi...

2020-06-03T05:28:50Z

New page: '''Unreleased structure''' The entry 7c8a is ON HOLD Authors: Himiyama, T., Nakamura, T. Description: Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modifi...

New page

'''Unreleased structure'''

The entry 7c8a is ON HOLD

Authors: Himiyama, T., Nakamura, T.

Description: Peroxiredoxin from Aeropyrum pernix K1 (ApPrx) C50S/F80C/C207S/C213S mutant modified with 2-(bromoacetyl)naphthalene(Naph@ApPrx*)
[[Category: Unreleased Structures]]
[[Category: Himiyama, T]]
[[Category: Nakamura, T]]