7cbb - Revision history

OCA at 10:54, 23 October 2024

2024-10-23T10:54:59Z

← Older revision		Revision as of 10:54, 23 October 2024
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	<StructureSection load='7cbb' size='340' side='right'caption='[[7cbb]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='7cbb' size='340' side='right'caption='[[7cbb]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7cbb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7CBB FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CBB FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~MSE~~:~~SELENOMETHIONINE~~'>~~MSE~~</scene><~~/td></tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">sbnC, DDL17_05250, M1K003_0650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])~~</td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbb OCA], [https://pdbe.org/7cbb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cbb RCSB], [https://www.ebi.ac.uk/pdbsum/7cbb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbb ProSAT]</span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7cbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbb OCA], [~~http~~://pdbe.org/7cbb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7cbb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7cbb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbb ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ju, Y]]		[[Category: Ju Y]]
	[[Category: Tang, J]]		[[Category: Tang J]]
	[[Category: Zhou, H]]		[[Category: Zhou H]]
	~~[[Category: Biosynthetic protein]]~~
	~~[[Category: Cupped hand fold]]~~
	~~[[Category: Siderophore synthetase]]~~
	~~[[Category: Type b nis synthetase~~]]

OCA at 20:55, 28 October 2020

2020-10-28T20:55:22Z

← Older revision		Revision as of 20:55, 28 October 2020
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	==Crystal structure of SbnC in the biosynthesis of staphyloferrin B==		==Crystal structure of SbnC in the biosynthesis of staphyloferrin B==
	<StructureSection load='7cbb' size='340' side='right'caption='[[7cbb]]' scene=''>		<StructureSection load='7cbb' size='340' side='right'caption='[[7cbb]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CBB FirstGlance]. <br>		<table><tr><td colspan='2'>[[7cbb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CBB FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbb OCA], [http://pdbe.org/7cbb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cbb RCSB], [http://www.ebi.ac.uk/pdbsum/7cbb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbb ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
			<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">sbnC, DDL17_05250, M1K003_0650 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbb OCA], [http://pdbe.org/7cbb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cbb RCSB], [http://www.ebi.ac.uk/pdbsum/7cbb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbb ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Staphyloferrin B is a key siderophore secreted by Staphylococcus aureus to acquire ferric ions from a host during infection, and its biosynthetic pathway has been validated to develop efficient antibacterial agents. Herein, we report the crystal structure of AMP-bound SbnC from S. aureus (SaSbnC) as the first representative structure of type B synthetases in the biosynthesis of alpha-hydroxycarboxylate siderophores. While type B synthetases specifically use alpha-ketoglutarate (alpha-KG) as their carboxylic acid substrate, SaSbnC showed unique structural features in the substrate pocket compared with the type A and C synthetases. Screening of alpha-KG analogues suggested that the hydrogen-bonding interaction between the alpha-carbonyl group of alpha-KG and residue Lys552 is a key determinant for the substrate selectivity of type B synthetases. Interestingly, citrate, the product of the tricarboxylic acid cycle and the substrate of type A synthetases, was found to inhibit the activity of SaSbnC with an IC50 value of 83 muM by mimicking alpha-KG binding, suggesting a potential regulatory role of the tricarboxylic acid cycle, whose activity is under the control of the intracellular iron concentration, to SaSbnC and other type B synthetases. These results provide critical new information to understand the structure, function, and regulation of type B synthetases in the siderophore-based iron acquisition system employed by a large number of pathogenic microbes.

			Structural and Biochemical Characterization of SbnC as a Representative Type B Siderophore Synthetase.,Tang J, Ju Y, Zhou J, Guo J, Gu Q, Xu J, Zhou H ACS Chem Biol. 2020 Sep 18. doi: 10.1021/acschembio.0c00523. PMID:32880431<ref>PMID:32880431</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7cbb" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Ju Y]]		[[Category: Ju, Y]]
	[[Category: Tang J]]		[[Category: Tang, J]]
	[[Category: Zhou H]]		[[Category: Zhou, H]]
			[[Category: Biosynthetic protein]]
			[[Category: Cupped hand fold]]
			[[Category: Siderophore synthetase]]
			[[Category: Type b nis synthetase]]

OCA at 11:16, 23 September 2020

2020-09-23T11:16:26Z

← Older revision		Revision as of 11:16, 23 September 2020
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7cbb is ~~ON HOLD until Paper Publication~~		==Crystal structure of SbnC in the biosynthesis of staphyloferrin B==
			<StructureSection load='7cbb' size='340' side='right'caption='[[7cbb]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CBB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CBB FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7cbb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cbb OCA], [http://pdbe.org/7cbb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7cbb RCSB], [http://www.ebi.ac.uk/pdbsum/7cbb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7cbb ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Ju Y]]
			[[Category: Tang J]]
			[[Category: Zhou H]]

OCA: Protected "7cbb" [edit=sysop:move=sysop]

2020-06-17T09:04:17Z

Protected "7cbb" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:04, 17 June 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7cbb is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2020-06-17T09:04:16Z

New page: '''Unreleased structure''' The entry 7cbb is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7cbb is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]