7cs2 - Revision history

OCA at 16:14, 29 November 2023

2023-11-29T16:14:42Z

← Older revision		Revision as of 16:14, 29 November 2023
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	==Apo structure of dimeric IiPLR1==		==Apo structure of dimeric IiPLR1==
	<StructureSection load='7cs2' size='340' side='right'caption='[[7cs2]]' scene=''>		<StructureSection load='7cs2' size='340' side='right'caption='[[7cs2]], [[Resolution\|resolution]] 2.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7cs2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Isatis_tinctoria Isatis tinctoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs2 OCA], [https://pdbe.org/7cs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs2 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs2 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6880047Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs2 OCA], [https://pdbe.org/7cs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs2 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/I6LRS1_ISATI I6LRS1_ISATI]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pinoresinol-lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis after the initial dimerization of two monolignols, and this represents the entry point for the synthesis of 8-8' lignans and contributes greatly to their structural diversity. Of particular interest has been the determination of how differing substrate specificities are achieved with these enzymes. Here, we present crystal structures of IiPLR1 from Isatis indigotica and pinoresinol reductases (PrRs) AtPrR1 and AtPrR2 from Arabidopsis thaliana, in the apo, substrate-bound and product-bound states. Each structure contains a head-to-tail homodimer, and the catalytic pocket comprises structural elements from both monomers. beta4 loop covers the top of the pocket, and residue 98 from the loop governs catalytic specificity. The substrate specificities of IiPLR1 and AtPrR2 can be switched via structure-guided mutagenesis. Our study provides insight into the molecular mechanism underlying the substrate specificity of PLRs/PrRs and suggests an efficient strategy for the large-scale commercial production of the pharmaceutically valuable compound lariciresinol.

			Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases.,Xiao Y, Shao K, Zhou J, Wang L, Ma X, Wu D, Yang Y, Chen J, Feng J, Qiu S, Lv Z, Zhang L, Zhang P, Chen W Nat Commun. 2021 May 14;12(1):2828. doi: 10.1038/s41467-021-23095-y. PMID:33990581<ref>PMID:33990581</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7cs2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Isatis tinctoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Shao K]]		[[Category: Shao K]]
	[[Category: Zhang P]]		[[Category: Zhang P]]

OCA at 12:12, 9 June 2021

2021-06-09T12:12:38Z

← Older revision		Revision as of 12:12, 9 June 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7cs2 is ~~ON HOLD until Paper Publication~~		==Apo structure of dimeric IiPLR1==
			<StructureSection load='7cs2' size='340' side='right'caption='[[7cs2]]' scene=''>
	~~Authors~~: ~~Shao~~, K., ~~Zhang~~, P.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS2 FirstGlance]. <br>
	~~Description: Apo structure of dimeric IiPLR1~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs2 OCA], [https://pdbe.org/7cs2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs2 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Zhang, P~~]]		__TOC__
	[[Category: ~~Shao, K~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Shao K]]
			[[Category: Zhang P]]

OCA at 11:02, 16 December 2020

2020-12-16T11:02:39Z

← Older revision		Revision as of 11:02, 16 December 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7cs2 is ON HOLD		The entry 7cs2 is ON HOLD until Paper Publication

	Authors: Shao, K., Zhang, P.		Authors: Shao, K., Zhang, P.

OCA at 05:11, 2 September 2020

2020-09-02T05:11:02Z

← Older revision		Revision as of 05:11, 2 September 2020
Line 5:		Line 5:
	Authors: Shao, K., Zhang, P.		Authors: Shao, K., Zhang, P.

	Description: ~~apo~~ structure of dimeric IiPLR1		Description: Apo structure of dimeric IiPLR1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Zhang, P]]
	[[Category: Shao, K]]		[[Category: Shao, K]]
	~~[[Category: Zhang, P]]~~

OCA: Protected "7cs2" [edit=sysop:move=sysop]

2020-08-26T09:24:02Z

Protected "7cs2" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:24, 26 August 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7cs2 is ON HOLD Authors: Shao, K., Zhang, P. Description: apo structure of dimeric IiPLR1 Category: Unreleased Structures Category: Shao, K...

2020-08-26T09:24:00Z

New page: '''Unreleased structure''' The entry 7cs2 is ON HOLD Authors: Shao, K., Zhang, P. Description: apo structure of dimeric IiPLR1 Category: Unreleased Structures Category: Shao, K...

New page

'''Unreleased structure'''

The entry 7cs2 is ON HOLD

Authors: Shao, K., Zhang, P.

Description: apo structure of dimeric IiPLR1
[[Category: Unreleased Structures]]
[[Category: Shao, K]]
[[Category: Zhang, P]]