7cs4 - Revision history

OCA at 16:14, 29 November 2023

2023-11-29T16:14:46Z

← Older revision		Revision as of 16:14, 29 November 2023
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	==IiPLR1 with NADP+ and (+)pinoresinol==		==IiPLR1 with NADP+ and (+)pinoresinol==
	<StructureSection load='7cs4' size='340' side='right'caption='[[7cs4]]' scene=''>		<StructureSection load='7cs4' size='340' side='right'caption='[[7cs4]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7cs4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Isatis_tinctoria Isatis tinctoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS4 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs4 OCA], [https://pdbe.org/7cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs4 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3050926Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GEC:4-[(3S,3aR,6S,6aR)-6-(3-methoxy-4-oxidanyl-phenyl)-1,3,3a,4,6,6a-hexahydrofuro[3,4-c]furan-3-yl]-2-methoxy-phenol'>GEC</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs4 OCA], [https://pdbe.org/7cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs4 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/I6LRS1_ISATI I6LRS1_ISATI]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Pinoresinol-lariciresinol reductases (PLRs) are enzymes involved in the lignan biosynthesis after the initial dimerization of two monolignols, and this represents the entry point for the synthesis of 8-8' lignans and contributes greatly to their structural diversity. Of particular interest has been the determination of how differing substrate specificities are achieved with these enzymes. Here, we present crystal structures of IiPLR1 from Isatis indigotica and pinoresinol reductases (PrRs) AtPrR1 and AtPrR2 from Arabidopsis thaliana, in the apo, substrate-bound and product-bound states. Each structure contains a head-to-tail homodimer, and the catalytic pocket comprises structural elements from both monomers. beta4 loop covers the top of the pocket, and residue 98 from the loop governs catalytic specificity. The substrate specificities of IiPLR1 and AtPrR2 can be switched via structure-guided mutagenesis. Our study provides insight into the molecular mechanism underlying the substrate specificity of PLRs/PrRs and suggests an efficient strategy for the large-scale commercial production of the pharmaceutically valuable compound lariciresinol.

			Structure-based engineering of substrate specificity for pinoresinol-lariciresinol reductases.,Xiao Y, Shao K, Zhou J, Wang L, Ma X, Wu D, Yang Y, Chen J, Feng J, Qiu S, Lv Z, Zhang L, Zhang P, Chen W Nat Commun. 2021 May 14;12(1):2828. doi: 10.1038/s41467-021-23095-y. PMID:33990581<ref>PMID:33990581</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7cs4" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Isatis tinctoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Shao K]]		[[Category: Shao K]]
	[[Category: Zhang P]]		[[Category: Zhang P]]

OCA at 12:12, 9 June 2021

2021-06-09T12:12:41Z

← Older revision		Revision as of 12:12, 9 June 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7cs4 is ~~ON HOLD until Paper Publication~~		==IiPLR1 with NADP+ and (+)pinoresinol==
			<StructureSection load='7cs4' size='340' side='right'caption='[[7cs4]]' scene=''>
	~~Authors~~: ~~Shao~~, K., ~~Zhang~~, P.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CS4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CS4 FirstGlance]. <br>
	~~Description: IiPLR1 with NADP+ and (+)pinoresinol~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7cs4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7cs4 OCA], [https://pdbe.org/7cs4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7cs4 RCSB], [https://www.ebi.ac.uk/pdbsum/7cs4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7cs4 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Shao, K]]		__TOC__
	[[Category: Zhang, P]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Shao K]]
			[[Category: Zhang P]]

OCA at 06:41, 21 October 2020

2020-10-21T06:41:37Z

← Older revision		Revision as of 06:41, 21 October 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7cs4 is ON HOLD		The entry 7cs4 is ON HOLD until Paper Publication

	Authors: Shao, K., Zhang, P.		Authors: Shao, K., Zhang, P.

OCA: Protected "7cs4" [edit=sysop:move=sysop]

2020-08-26T09:24:12Z

Protected "7cs4" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:24, 26 August 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7cs4 is ON HOLD Authors: Shao, K., Zhang, P. Description: IiPLR1 with NADP+ and (+)pinoresinol Category: Unreleased Structures [[Category: Shao...

2020-08-26T09:24:10Z

New page: '''Unreleased structure''' The entry 7cs4 is ON HOLD Authors: Shao, K., Zhang, P. Description: IiPLR1 with NADP+ and (+)pinoresinol Category: Unreleased Structures [[Category: Shao...

New page

'''Unreleased structure'''

The entry 7cs4 is ON HOLD

Authors: Shao, K., Zhang, P.

Description: IiPLR1 with NADP+ and (+)pinoresinol
[[Category: Unreleased Structures]]
[[Category: Shao, K]]
[[Category: Zhang, P]]