7css - Revision history

OCA at 06:11, 21 November 2024

2024-11-21T06:11:13Z

← Older revision		Revision as of 06:11, 21 November 2024
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	<StructureSection load='7css' size='340' side='right'caption='[[7css]]' scene=''>		<StructureSection load='7css' size='340' side='right'caption='[[7css]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7css]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli].~~ Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSS FirstGlance]. <br>		<table><tr><td colspan='2'>Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSS FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7css FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7css OCA], [https://pdbe.org/7css PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7css RCSB], [https://www.ebi.ac.uk/pdbsum/7css PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7css ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7css FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7css OCA], [https://pdbe.org/7css PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7css RCSB], [https://www.ebi.ac.uk/pdbsum/7css PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7css ProSAT]</span></td></tr>
	</table>		</table>
	~~== Function ==~~
	~~[https://www.uniprot.org/uniprot/HST3_ECOLX HST3_ECOLX] Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells.~~
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Escherichia coli]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hidaka Y]]		[[Category: Hidaka Y]]
	[[Category: Shimamoto S]]		[[Category: Shimamoto S]]

OCA at 11:12, 14 June 2023

2023-06-14T11:12:19Z

← Older revision		Revision as of 11:12, 14 June 2023
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	<StructureSection load='7css' size='340' side='right'caption='[[7css]]' scene=''>		<StructureSection load='7css' size='340' side='right'caption='[[7css]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=7CSS FirstGlance]. <br>		<table><tr><td colspan='2'>[[7css]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CSS FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=7css FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7css OCA], [~~http~~://pdbe.org/7css PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=7css RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/7css PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=7css ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7css FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7css OCA], [https://pdbe.org/7css PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7css RCSB], [https://www.ebi.ac.uk/pdbsum/7css PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7css ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/HST3_ECOLX HST3_ECOLX] Toxin which activates the particulate form of guanylate cyclase and increases cyclic GMP levels within the host intestinal epithelial cells.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Heat-stable enterotoxin (STa) produced by enterotoxigenic E. coli causes acute diarrhea and also can be used as a specific probe for colorectal cancer cells. STa contains three intra-molecular disulfide bonds (C1-C4, C2-C5, and C3-C6 connectivity). The chemical synthesis of STa provided not only the native type of STa but also a topological isomer that had the native disulfide pairings. Interestingly, the activity of the topological isomer was approximately 1/10-1/2 that of the native STa. To further investigate the bioactive conformation of this molecule and the regulation of disulfide-coupled folding during its chemical syntheses, we examined the folding mechanism of STa that occurs during its chemical synthesis. The folding intermediate of STa with two disulfide bonds (C1-C4 and C3-C6) and two Cys(Acm) residues, the precursor peptide, was treated with iodine to produce a third disulfide bond under several conditions. The topological isomer was predominantly produced under all conditions tested, along with trace amounts of the native type of STa. In addition, NMR measurements indicated that the topological isomer has a left-handed spiral structure similar to that of the precursor peptide, while the native type of STa had a right-handed spiral structure. These results indicate that the order of the regioselective formation of disulfide bonds is important for the regulation of the final conformation of disulfide-rich peptides in chemical synthesis.

			Topological Regulation of the Bioactive Conformation of a Disulfide-Rich Peptide, Heat-Stable Enterotoxin.,Shimamoto S, Fukutsuji M, Osumi T, Goto M, Toyoda H, Hidaka Y Molecules. 2020 Oct 21;25(20). pii: molecules25204798. doi:, 10.3390/molecules25204798. PMID:33096591<ref>PMID:33096591</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7css" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hidaka Y]]		[[Category: Hidaka Y]]
	[[Category: Shimamoto S]]		[[Category: Shimamoto S]]

OCA at 12:35, 16 December 2020

2020-12-16T12:35:08Z

← Older revision		Revision as of 12:35, 16 December 2020
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	~~'''Unreleased structure'''~~

	~~The entry 7css is ON HOLD until Aug 17 2022~~		==Solution structure of the topological isomer of Heat-stable enterotoxin produced by Enterotoxigenic Escherichia coli==
			<StructureSection load='7css' size='340' side='right'caption='[[7css]]' scene=''>
	~~Authors: Shimamoto, S., Hidaka, Y.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CSS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7CSS FirstGlance]. <br>
	~~Description:~~ Solution structure of the topological isomer of Heat-stable enterotoxin produced by Enterotoxigenic Escherichia coli		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7css FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7css OCA], [http://pdbe.org/7css PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7css RCSB], [http://www.ebi.ac.uk/pdbsum/7css PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7css ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Shimamoto, S~~]]		__TOC__
	[[Category: ~~Hidaka, Y~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Hidaka Y]]
			[[Category: Shimamoto S]]

OCA at 04:08, 14 October 2020

2020-10-14T04:08:21Z

← Older revision		Revision as of 04:08, 14 October 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7css is ON HOLD		The entry 7css is ON HOLD until Aug 17 2022

	Authors: Shimamoto, S., Hidaka, Y.		Authors: Shimamoto, S., Hidaka, Y.

OCA: Protected "7css" [edit=sysop:move=sysop]

2020-08-26T09:26:07Z

Protected "7css" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:26, 26 August 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7css is ON HOLD Authors: Shimamoto, S., Hidaka, Y. Description: Solution structure of the topological isomer of Heat-stable enterotoxin produced by...

2020-08-26T09:26:05Z

New page: '''Unreleased structure''' The entry 7css is ON HOLD Authors: Shimamoto, S., Hidaka, Y. Description: Solution structure of the topological isomer of Heat-stable enterotoxin produced by...

New page

'''Unreleased structure'''

The entry 7css is ON HOLD

Authors: Shimamoto, S., Hidaka, Y.

Description: Solution structure of the topological isomer of Heat-stable enterotoxin produced by Enterotoxigenic Escherichia coli
[[Category: Unreleased Structures]]
[[Category: Shimamoto, S]]
[[Category: Hidaka, Y]]