7ctr - Revision history

OCA at 16:16, 29 November 2023

2023-11-29T16:16:11Z

← Older revision		Revision as of 16:16, 29 November 2023
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	<StructureSection load='7ctr' size='340' side='right'caption='[[7ctr]], [[Resolution\|resolution]] 1.20Å' scene=''>		<StructureSection load='7ctr' size='340' side='right'caption='[[7ctr]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ctr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"saccharomonospora_internatus"_(agre_et_al._1974)_greiner-mai_et_al._1988 "saccharomonospora internatus" (agre et al. 1974) greiner-mai et al. 1988~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ctr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomonospora_viridis Saccharomonospora viridis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTR FirstGlance]. <br>
	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=DIO:~~1~~,4-DIETHYLENE+DIOXIDE'>DIO</scene>~~</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.2Å</td></tr>
	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>Cut190, SAMN02982918_2340 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1852~~ "~~Saccharomonospora internatus~~" ~~(Agre et al. 1974) Greiner-Mai et al. 1988])</td~~><~~/tr>~~		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[https://en.wikipedia.org/wiki/Cutinase Cutinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.74 3.1.1.74]~~ </~~span~~></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctr OCA], [https://pdbe.org/7ctr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctr RCSB], [https://www.ebi.ac.uk/pdbsum/7ctr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctr ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctr OCA], [https://pdbe.org/7ctr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctr RCSB], [https://www.ebi.ac.uk/pdbsum/7ctr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctr ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/W0TJ64_9PSEU W0TJ64_9PSEU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7ctr" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7ctr" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Cutinase 3D structures\|Cutinase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Cutinase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bekker~~, G J~~]]		[[Category: Saccharomonospora viridis]]
	[[Category: Emori, M]]		[[Category: Bekker GJ]]
	[[Category: Ito, N]]		[[Category: Emori M]]
	[[Category: Kamiya, N]]		[[Category: Ito N]]
	[[Category: Kawai, F]]		[[Category: Kamiya N]]
	[[Category: Numoto, N]]		[[Category: Kawai F]]
	[[Category: Oda, M]]		[[Category: Numoto N]]
	[[Category: Senga, A]]		[[Category: Oda M]]
	~~[[Category: Disulfide bond]]~~		[[Category: Senga A]]
	~~[[Category: Hydrolase]]~~
	~~[[Category: Metal binding]]~~
	~~[[Category: Polyesterase]]~~
	~~[[Category: Protein engineering~~]]

OCA at 07:02, 14 April 2021

2021-04-14T07:02:00Z

← Older revision		Revision as of 07:02, 14 April 2021
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	==Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H==		==Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H==
	<StructureSection load='7ctr' size='340' side='right'caption='[[7ctr]]' scene=''>		<StructureSection load='7ctr' size='340' side='right'caption='[[7ctr]], [[Resolution\|resolution]] 1.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTR FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ctr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"saccharomonospora_internatus"_(agre_et_al._1974)_greiner-mai_et_al._1988 "saccharomonospora internatus" (agre et al. 1974) greiner-mai et al. 1988]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctr OCA], [https://pdbe.org/7ctr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctr RCSB], [https://www.ebi.ac.uk/pdbsum/7ctr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctr ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DIO:1,4-DIETHYLENE+DIOXIDE'>DIO</scene></td></tr>
			<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Cut190, SAMN02982918_2340 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1852 "Saccharomonospora internatus" (Agre et al. 1974) Greiner-Mai et al. 1988])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Cutinase Cutinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.74 3.1.1.74] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctr OCA], [https://pdbe.org/7ctr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctr RCSB], [https://www.ebi.ac.uk/pdbsum/7ctr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctr ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The cutinase-like enzyme from the thermophile Saccharomonospora viridis AHK190, Cut190, is a good candidate to depolymerize polyethylene terephthalate (PET) efficiently. We previously developed a mutant of Cut190 (S226P/R228S), which we designated as Cut190* that has both increased activity and stability and solved its crystal structure. Recently, we showed that mutation of D250C/E296C on one of the Ca(2+) -binding sites resulted in a higher thermal stability while retaining its polyesterase activity. In this study, we solved the crystal structures of Cut190* mutants, Q138A/D250C-E296C/Q123H/N202H, designated as Cut190SS, and its inactive S176A mutant, Cut190SS_S176A, at high resolution. The overall structures were similar to those of Cut190* and Cut190S176A reported previously. As expected, Cys250 and Cys296 were closely located to form a disulfide bond, which would assuredly contribute to increase the stability. Isothermal titration calorimetry experiments and 3D Reference Interaction Site Model calculations showed that the metal-binding properties of the Cut190SS series were different from those of the Cut190* series. However, our results show that binding of Ca(2+) to the weak binding site, site 1, would be retained, enabling Cut190SS to keep its ability to use Ca(2+) to accelerate the conformational change from the closed (inactive) to the open (active) form. While increasing the thermal stability, Cut190SS could still express its enzymatic function. Even after incubation at 70 degrees C, which corresponds to the glass transition temperature of PET, the enzyme retained its activity well, implying a high applicability for industrial PET depolymerization using Cut190*SS.

			Structural basis of mutants of PET-degrading enzyme from Saccharomonospora viridis AHK190 with high activity and thermal stability.,Emori M, Numoto N, Senga A, Bekker GJ, Kamiya N, Kobayashi Y, Ito N, Kawai F, Oda M Proteins. 2020 Dec 19. doi: 10.1002/prot.26034. PMID:33340163<ref>PMID:33340163</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ctr" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Cutinase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bekker GJ]]		[[Category: Bekker, G J]]
	[[Category: Emori M]]		[[Category: Emori, M]]
	[[Category: Ito N]]		[[Category: Ito, N]]
	[[Category: Kamiya N]]		[[Category: Kamiya, N]]
	[[Category: Kawai F]]		[[Category: Kawai, F]]
	[[Category: Numoto N]]		[[Category: Numoto, N]]
	[[Category: Oda M]]		[[Category: Oda, M]]
	[[Category: Senga A]]		[[Category: Senga, A]]
			[[Category: Disulfide bond]]
			[[Category: Hydrolase]]
			[[Category: Metal binding]]
			[[Category: Polyesterase]]
			[[Category: Protein engineering]]

OCA at 11:10, 3 February 2021

2021-02-03T11:10:45Z

← Older revision		Revision as of 11:10, 3 February 2021
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	~~'''Unreleased structure'''~~

	~~The entry 7ctr is ON HOLD until Paper Publication~~		==Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H==
			<StructureSection load='7ctr' size='340' side='right'caption='[[7ctr]]' scene=''>
	~~Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CTR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CTR FirstGlance]. <br>
	~~Description:~~ Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ctr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ctr OCA], [https://pdbe.org/7ctr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ctr RCSB], [https://www.ebi.ac.uk/pdbsum/7ctr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ctr ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Ito, N~~]]		__TOC__
	[[Category: ~~Kamiya, N~~]]		</StructureSection>
	[[Category: ~~Emori, M~~]]		[[Category: Large Structures]]
	[[Category: ~~Numoto,~~ N]]		[[Category: Bekker GJ]]
	[[Category: ~~Bekker, G.J~~]]		[[Category: Emori M]]
	[[Category: ~~Oda, M~~]]		[[Category: Ito N]]
	[[Category: ~~Kawai, F~~]]		[[Category: Kamiya N]]
	[[Category: Senga, A]]		[[Category: Kawai F]]
			[[Category: Numoto N]]
			[[Category: Oda M]]
			[[Category: Senga A]]

OCA at 07:31, 9 September 2020

2020-09-09T07:31:10Z

← Older revision		Revision as of 07:31, 9 September 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ctr is ON HOLD		The entry 7ctr is ON HOLD until Paper Publication

	Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M.		Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M.
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	Description: Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H		Description: Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: ~~Senga~~, A]]		[[Category: Ito, N]]
	[[Category: Kamiya, N]]		[[Category: Kamiya, N]]
			[[Category: Emori, M]]
	[[Category: Numoto, N]]		[[Category: Numoto, N]]
	[[Category: Bekker, G.J]]		[[Category: Bekker, G.J]]
	[[Category: ~~Ito, N]]~~		[[Category: Oda, M]]
	~~[[Category: Emori~~, M]]
	[[Category: Kawai, F]]		[[Category: Kawai, F]]
	[[Category: ~~Oda~~, M]]		[[Category: Senga, A]]

OCA: Protected "7ctr" [edit=sysop:move=sysop]

2020-08-26T09:29:07Z

Protected "7ctr" [edit=sysop:move=sysop]

← Older revision	Revision as of 09:29, 26 August 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ctr is ON HOLD Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M. Description: Closed form of PET-de...

2020-08-26T09:29:05Z

New page: '''Unreleased structure''' The entry 7ctr is ON HOLD Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M. Description: Closed form of PET-de...

New page

'''Unreleased structure'''

The entry 7ctr is ON HOLD

Authors: Emori, M., Numoto, N., Senga, A., Bekker, G.J., Kamiya, N., Ito, N., Kawai, F., Oda, M.

Description: Closed form of PET-degrading cutinase Cut190 with thermostability-improving mutations of S226P/R228S/Q138A/D250C-E296C/Q123H/N202H
[[Category: Unreleased Structures]]
[[Category: Senga, A]]
[[Category: Kamiya, N]]
[[Category: Numoto, N]]
[[Category: Bekker, G.J]]
[[Category: Ito, N]]
[[Category: Emori, M]]
[[Category: Kawai, F]]
[[Category: Oda, M]]