7d2f - Revision history

OCA at 16:24, 29 November 2023

2023-11-29T16:24:21Z

← Older revision		Revision as of 16:24, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7d2f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>		<table><tr><td colspan='2'>[[7d2f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q5ZWG6_LEGPH Q5ZWG6_LEGPH]]		[https://www.uniprot.org/uniprot/Q5ZWG6_LEGPH Q5ZWG6_LEGPH]

	==See Also==		==See Also==

OCA at 07:12, 8 September 2022

2022-09-08T07:12:32Z

← Older revision		Revision as of 07:12, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7d2f' size='340' side='right'caption='[[7d2f]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='7d2f' size='340' side='right'caption='[[7d2f]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7d2f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>		<table><tr><td colspan='2'>[[7d2f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~		== Function ==
	== ~~Publication Abstract from PubMed~~ ==		[[https://www.uniprot.org/uniprot/Q5ZWG6_LEGPH Q5ZWG6_LEGPH]]
	MapA is a histidine acid phosphatase (HAP) from Legionella pneumophila that catalyzes the hydroxylation of a phosphoryl group from phosphomonoesters by an active-site histidine. Several structures of HAPs, including MapA, in complex with the inhibitor tartrate have been solved and the substrate binding tunnel identified; however, the substrate recognition mechanism remains unknown. To gain insight into the mechanism of substrate recognition, the crystal structures of apo-MapA and the MapA(D281A) mutant in complex with 5'-AMP were solved at 2.2 and 2.~~6 A resolution, respectively~~. ~~The structure of the MapA(D281A)~~/5'-AMP complex reveals that the 5'-AMP fits fully into the substrate binding tunnel, with the 2'-hydroxyl group of the ribose moiety stabilized by Glu201 and the adenine moiety sandwiched between His205 and Phe237. This is the second structure of a HAP/~~AMP complex solved with 5'-AMP binding in a unique manner in the active site. The structure presents a new substrate recognition mechanism of HAPs.~~

	Structural insights into a new substrate binding mode of a histidine acid phosphatase from Legionella pneumophila.,Guo Y, Zhou D, Zhang H, Zhang NN, Qi X, Chen X, Chen Q, Li J, Ge H, Teng YB Biochem Biophys Res Commun. 2021 Feb 12;540:90-94. doi:, 10.1016/j.bbrc.2020.12.070. Epub 2021 Jan 12. PMID:33450485<ref>PMID:33450485</ref>		==See Also==
			*[[Acid phosphatase 3D structures\|Acid phosphatase 3D structures]]
	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7d2f" style~~=~~"background-color:#fffaf0;"></div>~~
	=~~= References~~ ==
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Acid phosphatase]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Guo, Y~~]]		[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
	[[Category: ~~Teng,~~ Y]]		[[Category: Guo Y]]
	[[Category: ~~5'-amp]]~~		[[Category: Teng Y]]
	~~[[Category: Complex]]~~
	~~[[Category: Hydrolase~~]]

OCA at 16:19, 6 July 2022

2022-07-06T16:19:46Z

← Older revision		Revision as of 16:19, 6 July 2022
Line 1:		Line 1:

	==Lp major histidine acid phosphatase mutant D281A/5'-AMP==		==Lp major histidine acid phosphatase mutant D281A/5'-AMP==
	<StructureSection load='7d2f' size='340' side='right'caption='[[7d2f]]' scene=''>		<StructureSection load='7d2f' size='340' side='right'caption='[[7d2f]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>		<table><tr><td colspan='2'>[[7d2f]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acid_phosphatase Acid phosphatase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.2 3.1.3.2] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			MapA is a histidine acid phosphatase (HAP) from Legionella pneumophila that catalyzes the hydroxylation of a phosphoryl group from phosphomonoesters by an active-site histidine. Several structures of HAPs, including MapA, in complex with the inhibitor tartrate have been solved and the substrate binding tunnel identified; however, the substrate recognition mechanism remains unknown. To gain insight into the mechanism of substrate recognition, the crystal structures of apo-MapA and the MapA(D281A) mutant in complex with 5'-AMP were solved at 2.2 and 2.6 A resolution, respectively. The structure of the MapA(D281A)/5'-AMP complex reveals that the 5'-AMP fits fully into the substrate binding tunnel, with the 2'-hydroxyl group of the ribose moiety stabilized by Glu201 and the adenine moiety sandwiched between His205 and Phe237. This is the second structure of a HAP/AMP complex solved with 5'-AMP binding in a unique manner in the active site. The structure presents a new substrate recognition mechanism of HAPs.

			Structural insights into a new substrate binding mode of a histidine acid phosphatase from Legionella pneumophila.,Guo Y, Zhou D, Zhang H, Zhang NN, Qi X, Chen X, Chen Q, Li J, Ge H, Teng YB Biochem Biophys Res Commun. 2021 Feb 12;540:90-94. doi:, 10.1016/j.bbrc.2020.12.070. Epub 2021 Jan 12. PMID:33450485<ref>PMID:33450485</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7d2f" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Acid phosphatase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Guo Y]]		[[Category: Guo, Y]]
	[[Category: Teng Y]]		[[Category: Teng, Y]]
			[[Category: 5'-amp]]
			[[Category: Complex]]
			[[Category: Hydrolase]]

OCA at 06:48, 22 September 2021

2021-09-22T06:48:58Z

← Older revision		Revision as of 06:48, 22 September 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7d2f is ~~ON HOLD until Paper Publication~~		==Lp major histidine acid phosphatase mutant D281A/5'-AMP==
			<StructureSection load='7d2f' size='340' side='right'caption='[[7d2f]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D2F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D2F FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d2f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d2f OCA], [https://pdbe.org/7d2f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d2f RCSB], [https://www.ebi.ac.uk/pdbsum/7d2f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d2f ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Guo Y]]
			[[Category: Teng Y]]

OCA at 06:23, 11 November 2020

2020-11-11T06:23:08Z

← Older revision		Revision as of 06:23, 11 November 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7d2f is ON HOLD		The entry 7d2f is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7d2f" [edit=sysop:move=sysop]

2020-09-23T08:58:37Z

Protected "7d2f" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:58, 23 September 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7d2f is ON HOLD Authors: Description: Category: Unreleased Structures

2020-09-23T08:58:36Z

New page: '''Unreleased structure''' The entry 7d2f is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7d2f is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]