OCA at 16:28, 29 November 2023

2023-11-29T16:28:19Z

← Older revision		Revision as of 16:28, 29 November 2023
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	====		==Crystal Structure of H3(1-13)/PHF14-PZP fusion protein==
	<StructureSection load='7d8a' size='340' side='right'caption='[[7d8a]]' scene=''>		<StructureSection load='7d8a' size='340' side='right'caption='[[7d8a]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7d8a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Danio_rerio Danio rerio] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7D8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7D8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8a OCA], [https://pdbe.org/7d8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8a RCSB], [https://www.ebi.ac.uk/pdbsum/7d8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8a ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8a OCA], [https://pdbe.org/7d8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8a RCSB], [https://www.ebi.ac.uk/pdbsum/7d8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8a ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PHF14_DANRE PHF14_DANRE] Histone-binding protein (PubMed:34365506). Binds preferentially to unmodified histone H3 but can also bind to a lesser extent to histone H3 trimethylated at 'Lys-9' (H3K9me3) as well as to histone H3 monomethylated at 'Lys-27' (H3K27ac) and trimethylated at 'Lys-27' (H3K27me3) (PubMed:34365506). Represses PDGFRA expression, thus playing a role in regulation of mesenchymal cell proliferation (By similarity).[UniProtKB:Q9D4H9]<ref>PMID:34365506</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Histone recognition constitutes a key epigenetic mechanism in gene regulation and cell fate decision. PHF14 is a conserved multi-PHD finger protein that has been implicated in organ development, tissue homeostasis, and tumorigenesis. Here we show that PHF14 reads unmodified histone H3(1-34) through an integrated PHD1-ZnK-PHD2 cassette (PHF14PZP). Our binding, structural and HDX-MS analyses revealed a feature of bipartite recognition, in which PHF14PZP utilizes two distinct surfaces for concurrent yet separable engagement of segments H3-Nter (e.g. 1-15) and H3-middle (e.g. 14-34) of H3(1-34). Structural studies revealed a novel histone H3 binding mode by PHD1 of PHF14PZP, in which a PHF14-unique insertion loop but not the core beta-strands of a PHD finger dominates H3K4 readout. Binding studies showed that H3-PHF14PZP engagement is sensitive to modifications occurring to H3 R2, T3, K4, R8 and K23 but not K9 and K27, suggesting multiple layers of modification switch. Collectively, our work calls attention to PHF14 as a 'ground' state (unmodified) H3(1-34) reader that can be negatively regulated by active marks, thus providing molecular insights into a repressive function of PHF14 and its derepression.

			Molecular basis for bipartite recognition of histone H3 by the PZP domain of PHF14.,Zheng S, Bi Y, Chen H, Gong B, Jia S, Li H Nucleic Acids Res. 2021 Sep 7;49(15):8961-8973. doi: 10.1093/nar/gkab670. PMID:34365506<ref>PMID:34365506</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7d8a" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Danio rerio]]
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Li H]]
			[[Category: Zheng S]]

OCA at 10:32, 28 July 2021

2021-07-28T10:32:27Z

← Older revision		Revision as of 10:32, 28 July 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7d8a is ~~ON HOLD until Paper Publication~~		====
			<StructureSection load='7d8a' size='340' side='right'caption='[[7d8a]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7d8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7d8a OCA], [https://pdbe.org/7d8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7d8a RCSB], [https://www.ebi.ac.uk/pdbsum/7d8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7d8a ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 08:36, 19 May 2021

2021-05-19T08:36:56Z

← Older revision		Revision as of 08:36, 19 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7d8a is ON HOLD		The entry 7d8a is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7d8a" [edit=sysop:move=sysop]

2020-10-14T04:13:18Z

Protected "7d8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 04:13, 14 October 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7d8a is ON HOLD Authors: Description: Category: Unreleased Structures

2020-10-14T04:13:17Z

New page: '''Unreleased structure''' The entry 7d8a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7d8a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7d8a - Revision history

OCA at 16:28, 29 November 2023

OCA at 10:32, 28 July 2021

OCA at 08:36, 19 May 2021

OCA: Protected "7d8a" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7d8a is ON HOLD Authors: Description: Category: Unreleased Structures