OCA at 05:55, 17 September 2025

2025-09-17T05:55:45Z

← Older revision		Revision as of 05:55, 17 September 2025
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	====		==Activity optimized supercomplex state4==
	<StructureSection load='7dkf' size='340' side='right'caption='[[7dkf]]' scene=''>		<StructureSection load='7dkf' size='340' side='right'caption='[[7dkf]], [[Resolution\|resolution]] 8.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dkf]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DKF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DKF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dkf OCA], [https://pdbe.org/7dkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dkf RCSB], [https://www.ebi.ac.uk/pdbsum/7dkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dkf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 8.3Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3PE:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>3PE</scene>, <scene name='pdbligand=CDL:CARDIOLIPIN'>CDL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=HEA:HEME-A'>HEA</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dkf OCA], [https://pdbe.org/7dkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dkf RCSB], [https://www.ebi.ac.uk/pdbsum/7dkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dkf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/QCR1_BOVIN QCR1_BOVIN] This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Aims: Mitochondrial respiratory supercomplexes mediate redox electron transfer, generating a proton gradient for ATP synthesis. To provide structural information on the function of supercomplexes in physiologically relevant conditions, we conducted cryoelectron microscopy studies with supercomplexes in a lipid-preserving state. Results: Here, we present cryoelectron microscopy structures of bovine respiratory supercomplex I(1)III(2)IV(1) by using a lipid-preserving sample preparation. The preparation greatly enhances the intercomplex quinone transfer activity. The structures reveal large intercomplex motions that result in different shapes and sizes of the intercomplex space between complexes I and III, forming a dynamic substrate pool. Biochemical and structural analyses indicated that intercomplex phospholipids mediate the intercomplex motions. An analysis of the different classes of focus-refined complex I showed that structural switches due to quinone reduction led to the formation of a novel channel that could transfer reduced quinones to the intercomplex substrate pool. Innovation and Conclusion: Our results indicate potential mechanism for the facilitated electron transfer involving a dynamic substrate pool and intercomplex movement by which supercomplexes play an active role in the regulation of metabolic flux and reactive oxygen species.

			A Dynamic Substrate Pool Revealed by cryo-EM of a Lipid-Preserved Respiratory Supercomplex.,Jeon TJ, Lee SG, Yoo SH, Kim M, Song D, Ryu J, Park H, Kim DS, Hyun J, Kim HM, Ryu SE Antioxid Redox Signal. 2022 Apr 27. doi: 10.1089/ars.2021.0114. PMID:34913730<ref>PMID:34913730</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7dkf" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Hyun JK]]
			[[Category: Jeon TJ]]
			[[Category: Kim DS]]
			[[Category: Kim HM]]
			[[Category: Lee SG]]
			[[Category: Ryu JH]]
			[[Category: Ryu SE]]
			[[Category: Yoo SH]]

OCA at 10:13, 18 May 2022

2022-05-18T10:13:47Z

← Older revision		Revision as of 10:13, 18 May 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7dkf is ~~ON HOLD until May 12 2023~~		====
			<StructureSection load='7dkf' size='340' side='right'caption='[[7dkf]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dkf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dkf OCA], [https://pdbe.org/7dkf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dkf RCSB], [https://www.ebi.ac.uk/pdbsum/7dkf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dkf ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 12:36, 3 November 2021

2021-11-03T12:36:09Z

← Older revision		Revision as of 12:36, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7dkf is ON HOLD until ~~Paper Publication~~		The entry 7dkf is ON HOLD until May 12 2023

	Authors:		Authors:

OCA at 13:21, 2 June 2021

2021-06-02T13:21:25Z

← Older revision		Revision as of 13:21, 2 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7dkf is ON HOLD		The entry 7dkf is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7dkf" [edit=sysop:move=sysop]

2020-12-02T05:42:59Z

Protected "7dkf" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:42, 2 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7dkf is ON HOLD Authors: Description: Category: Unreleased Structures

2020-12-02T05:42:58Z

New page: '''Unreleased structure''' The entry 7dkf is ON HOLD Authors: Description: Category: Unreleased Structures

New page