7dmo - Revision history

OCA at 16:36, 29 November 2023

2023-11-29T16:36:34Z

← Older revision		Revision as of 16:36, 29 November 2023
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	==Crystal structures of two pericyclases catalyzing [4+2] cycloadditions==		==Crystal structures of two pericyclases catalyzing [4+2] cycloadditions==
	<StructureSection load='7dmo' size='340' side='right'caption='[[7dmo]]' scene=''>		<StructureSection load='7dmo' size='340' side='right'caption='[[7dmo]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMO FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dmo]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrenochaetopsis_sp. Pyrenochaetopsis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmo OCA], [https://pdbe.org/7dmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmo RCSB], [https://www.ebi.ac.uk/pdbsum/7dmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmo ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmo OCA], [https://pdbe.org/7dmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmo RCSB], [https://www.ebi.ac.uk/pdbsum/7dmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmo ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/PHM7_PYRSX PHM7_PYRSX] Diels-Alderase; part of the gene cluster that mediates the biosynthesis of the trans-fused decalin-containing tetramic acid phomasetin, the stereochemical opposite of the HIV-1 integrase inhibitor equisetin (PubMed:29972614). The PKS module of phm1 together with the enoylreductase phm4 catalyze the formation of the polyketide unit which is then conjugated to L-serine by the condensation domain of the phm1 NRPS module (PubMed:29972614). Activity of the Dieckmann cyclase domain (RED) of phm1 results in release of the Dieckmann product intermediate (PubMed:29972614). The Diels-Alderase phm7 then uses the Dieckmann product of phm1 as substrate and catalyzes the Diels-Alder cycloaddition to form the decalin ring of N-desmethylphomasetin (PubMed:29972614, PubMed:34121297). N-desmethylphomasetin is further methylated to phomasetin by the methyltransferase phm5 (PubMed:29972614).<ref>PMID:29972614</ref> <ref>PMID:34121297</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Fsa2 and Phm7 are a unique pair of pericyclases catalyzing [4 + 2] cycloaddition reactions with reverse stereoselectivities in the biosynthesis of equisetin and phomasetin, both of which are potent HIV-1 integrase inhibitors. We here solve the crystal structures of Fsa2 and Phm7, both of which possess unusual "two-beta barrel" folds. Different residues are evident between the active sites of Fsa2 and Phm7, and modeling experiments provide key structural information determining the reverse stereoselectivities. These results provide a better understanding of how natural pericyclases control the catalytic stereoselectivities and benefit the protein engineering in future.

			Crystal Structures of Fsa2 and Phm7 Catalyzing [4 + 2] Cycloaddition Reactions with Reverse Stereoselectivities in Equisetin and Phomasetin Biosynthesis.,Chi C, Wang Z, Liu T, Zhang Z, Zhou H, Li A, Jin H, Jia H, Yin F, Yang D, Ma M ACS Omega. 2021 May 6;6(19):12913-12922. doi: 10.1021/acsomega.1c01593., eCollection 2021 May 18. PMID:34056443<ref>PMID:34056443</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7dmo" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Pyrenochaetopsis sp]]
	[[Category: Chi CB]]		[[Category: Chi CB]]
	[[Category: Ma M]]		[[Category: Ma M]]
	[[Category: Wang ZD]]		[[Category: Wang ZD]]

OCA at 05:29, 6 October 2021

2021-10-06T05:29:45Z

← Older revision		Revision as of 05:29, 6 October 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7dmo is ~~ON HOLD until Dec 04 2022~~		==Crystal structures of two pericyclases catalyzing [4+2] cycloadditions==
			<StructureSection load='7dmo' size='340' side='right'caption='[[7dmo]]' scene=''>
	~~Authors~~: ~~Wang~~, Z.D., ~~Chi~~, C.B., Ma, M.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DMO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DMO FirstGlance]. <br>
	~~Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dmo OCA], [https://pdbe.org/7dmo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dmo RCSB], [https://www.ebi.ac.uk/pdbsum/7dmo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dmo ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Wang, Z.D~~]]		__TOC__
	[[Category: ~~Chi, C.B~~]]		</StructureSection>
	[[Category: ~~Ma, M~~]]		[[Category: Large Structures]]
			[[Category: Chi CB]]
			[[Category: Ma M]]
			[[Category: Wang ZD]]

OCA at 13:14, 3 March 2021

2021-03-03T13:14:36Z

← Older revision		Revision as of 13:14, 3 March 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7dmo is ON HOLD		The entry 7dmo is ON HOLD until Dec 04 2022

	Authors: Wang, Z.D., Chi, C.B., Ma, M.		Authors: Wang, Z.D., Chi, C.B., Ma, M.

OCA at 11:06, 16 December 2020

2020-12-16T11:06:49Z

← Older revision		Revision as of 11:06, 16 December 2020
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	Authors: Wang, Z.D., Chi, C.B., Ma, M.		Authors: Wang, Z.D., Chi, C.B., Ma, M.

	Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions.		Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Wang, Z.D]]		[[Category: Wang, Z.D]]
	[[Category: Chi, C.B]]		[[Category: Chi, C.B]]
	[[Category: Ma, M]]		[[Category: Ma, M]]

OCA: Protected "7dmo" [edit=sysop:move=sysop]

2020-12-09T05:11:46Z

Protected "7dmo" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:11, 9 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7dmo is ON HOLD Authors: Wang, Z.D., Chi, C.B., Ma, M. Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions. [[Categ...

2020-12-09T05:11:45Z

New page: '''Unreleased structure''' The entry 7dmo is ON HOLD Authors: Wang, Z.D., Chi, C.B., Ma, M. Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions. [[Categ...

New page

'''Unreleased structure'''

The entry 7dmo is ON HOLD

Authors: Wang, Z.D., Chi, C.B., Ma, M.

Description: Crystal structures of two pericyclases catalyzing [4+2] cycloadditions.
[[Category: Unreleased Structures]]
[[Category: Wang, Z.D]]
[[Category: Chi, C.B]]
[[Category: Ma, M]]