7dqa - Revision history

OCA at 10:57, 23 October 2024

2024-10-23T10:57:53Z

← Older revision		Revision as of 10:57, 23 October 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7dqa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQA FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dqa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.8Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqa OCA], [https://pdbe.org/7dqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqa RCSB], [https://www.ebi.ac.uk/pdbsum/7dqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqa OCA], [https://pdbe.org/7dqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqa RCSB], [https://www.ebi.ac.uk/pdbsum/7dqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqa ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/~~SPIKE_SARS2 SPIKE_SARS2~~]~~] attaches the virion~~ to ~~the cell membrane by interacting with host receptor~~, ~~initiating the infection (By similarity)~~. ~~Binding~~ to ~~human ACE2 receptor~~ and ~~internalization~~ of ~~the virus into the endosomes~~ of ~~the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651~~, ~~PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2~~ for ~~priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask~~ the ~~fusion peptide~~ of ~~S2 and activate membranes fusion within endosomes~~.~~[HAMAP-Rule:MF_04099]~~<ref>PMID:~~32075877~~</ref> <ref>PMID:~~32142651~~</ref> <ref>PMID:~~32155444~~</ref> ~~mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre~~-~~fusion native state, pre~~-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] ~~Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099~~]		[https://www.uniprot.org/uniprot/ACE2_HUMAN ACE2_HUMAN] Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.<ref>PMID:10969042</ref> <ref>PMID:10924499</ref> <ref>PMID:14647384</ref>

			==See Also==
			*[[Angiotensin-Converting Enzyme 3D structures\|Angiotensin-Converting Enzyme 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:20, 8 September 2022

2022-09-08T06:20:21Z

← Older revision		Revision as of 06:20, 8 September 2022
Line 1:		Line 1:

	====		==Cryo-EM structure of SARS-CoV2 RBD-ACE2 complex==
	<StructureSection load='7dqa' size='340' side='right'caption='[[7dqa]]' scene=''>		<StructureSection load='7dqa' size='340' side='right'caption='[[7dqa]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dqa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DQA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqa OCA], [https://pdbe.org/7dqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqa RCSB], [https://www.ebi.ac.uk/pdbsum/7dqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqa ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqa OCA], [https://pdbe.org/7dqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqa RCSB], [https://www.ebi.ac.uk/pdbsum/7dqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqa ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/SPIKE_SARS2 SPIKE_SARS2]] attaches the virion to the cell membrane by interacting with host receptor, initiating the infection (By similarity). Binding to human ACE2 receptor and internalization of the virus into the endosomes of the host cell induces conformational changes in the Spike glycoprotein (PubMed:32142651, PubMed:32075877, PubMed:32155444). Uses also human TMPRSS2 for priming in human lung cells which is an essential step for viral entry (PubMed:32142651). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.[HAMAP-Rule:MF_04099]<ref>PMID:32075877</ref> <ref>PMID:32142651</ref> <ref>PMID:32155444</ref> mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. Under the current model, the protein has at least three conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes.[HAMAP-Rule:MF_04099] Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis.[HAMAP-Rule:MF_04099]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Severe acute respiratory syndrome coronavirus 2]]
			[[Category: Lan J]]
			[[Category: Wang HW]]
			[[Category: Wang J]]
			[[Category: Wang XQ]]

OCA at 14:07, 29 December 2021

2021-12-29T14:07:40Z

← Older revision		Revision as of 14:07, 29 December 2021
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7dqa ~~is ON HOLD~~		====
			<StructureSection load='7dqa' size='340' side='right'caption='[[7dqa]]' scene=''>
	~~Authors~~: ~~Wang, J~~.~~, Lan, J~~.~~, Wang, X~~.Q.~~, Wang, H~~.W.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Cryo-EM structure of SARS-CoV2 RBD-ACE2 complex~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dqa OCA], [https://pdbe.org/7dqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dqa RCSB], [https://www.ebi.ac.uk/pdbsum/7dqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dqa ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[~~Category~~: ~~Wang, H~~.W]]		__TOC__
	[[~~Category~~: ~~Lan, J]~~]		</StructureSection>
	[[Category: ~~Wang, X.Q~~]]		[[Category: Large Structures]]
	[[Category: ~~Wang, J~~]]		[[Category: Z-disk]]

OCA: Protected "7dqa" [edit=sysop:move=sysop]

2020-12-30T05:23:31Z

Protected "7dqa" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:23, 30 December 2020
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7dqa is ON HOLD Authors: Wang, J., Lan, J., Wang, X.Q., Wang, H.W. Description: Cryo-EM structure of SARS-CoV2 RBD-ACE2 complex [[Category: Unrelea...

2020-12-30T05:23:30Z

New page: '''Unreleased structure''' The entry 7dqa is ON HOLD Authors: Wang, J., Lan, J., Wang, X.Q., Wang, H.W. Description: Cryo-EM structure of SARS-CoV2 RBD-ACE2 complex [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 7dqa is ON HOLD

Authors: Wang, J., Lan, J., Wang, X.Q., Wang, H.W.

Description: Cryo-EM structure of SARS-CoV2 RBD-ACE2 complex
[[Category: Unreleased Structures]]
[[Category: Wang, H.W]]
[[Category: Lan, J]]
[[Category: Wang, X.Q]]
[[Category: Wang, J]]