OCA at 16:40, 29 November 2023

2023-11-29T16:40:30Z

← Older revision		Revision as of 16:40, 29 November 2023
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	==Crystal structure of Apo (heme-free) PefR==		==Crystal structure of Apo (heme-free) PefR==
	<StructureSection load='7dvs' size='340' side='right'caption='[[7dvs]]' scene=''>		<StructureSection load='7dvs' size='340' side='right'caption='[[7dvs]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVS FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dvs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae Streptococcus agalactiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVS FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvs OCA], [https://pdbe.org/7dvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvs RCSB], [https://www.ebi.ac.uk/pdbsum/7dvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvs ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvs OCA], [https://pdbe.org/7dvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvs RCSB], [https://www.ebi.ac.uk/pdbsum/7dvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvs ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/R4Z9I5_STRAG R4Z9I5_STRAG]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Hemes (iron-porphyrins) are critical for biological processes in all organisms. Hemolytic bacteria survive by acquiring b-type heme from hemoglobin in red blood cells from their animal hosts. These bacteria avoid the cytotoxicity of excess heme during hemolysis by expressing heme-responsive sensor proteins that act as transcriptional factors to regulate the heme efflux system in response to the cellular heme concentration. Here, the underlying regulatory mechanisms were investigated using crystallographic, spectroscopic, and biochemical studies to understand the structural basis of the heme-responsive sensor protein PefR from Streptococcus agalactiae, a causative agent of neonatal life-threatening infections. Structural comparison of heme-free PefR, its complex with a target DNA, and heme-bound PefR revealed that unique heme coordination controls a >20 A structural rearrangement of the DNA binding domains to dissociate PefR from the target DNA. We also found heme-bound PefR stably binds exogenous ligands, including carbon monoxide, a by-product of the heme degradation reaction.

			Heme controls the structural rearrangement of its sensor protein mediating the hemolytic bacterial survival.,Nishinaga M, Sugimoto H, Nishitani Y, Nagai S, Nagatoishi S, Muraki N, Tosha T, Tsumoto K, Aono S, Shiro Y, Sawai H Commun Biol. 2021 Apr 13;4(1):467. doi: 10.1038/s42003-021-01987-5. PMID:33850260<ref>PMID:33850260</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7dvs" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Streptococcus agalactiae]]
	[[Category: Aono S]]		[[Category: Aono S]]
	[[Category: Muraki N]]		[[Category: Muraki N]]

OCA at 08:48, 29 September 2021

2021-09-29T08:48:31Z

← Older revision		Revision as of 08:48, 29 September 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7dvs is ~~ON HOLD until Paper Publication~~		==Crystal structure of Apo (heme-free) PefR==
			<StructureSection load='7dvs' size='340' side='right'caption='[[7dvs]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DVS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DVS FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dvs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dvs OCA], [https://pdbe.org/7dvs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dvs RCSB], [https://www.ebi.ac.uk/pdbsum/7dvs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dvs ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Aono S]]
			[[Category: Muraki N]]

OCA at 06:38, 27 January 2021

2021-01-27T06:38:17Z

← Older revision		Revision as of 06:38, 27 January 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7dvs is ON HOLD		The entry 7dvs is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7dvs" [edit=sysop:move=sysop]

2021-01-20T06:49:59Z

Protected "7dvs" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:49, 20 January 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7dvs is ON HOLD Authors: Description: Category: Unreleased Structures

2021-01-20T06:49:58Z

New page: '''Unreleased structure''' The entry 7dvs is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7dvs is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7dvs - Revision history

OCA at 16:40, 29 November 2023

OCA at 08:48, 29 September 2021

OCA at 06:38, 27 January 2021

OCA: Protected "7dvs" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7dvs is ON HOLD Authors: Description: Category: Unreleased Structures