OCA at 10:54, 27 March 2024

2024-03-27T10:54:11Z

← Older revision		Revision as of 10:54, 27 March 2024
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	====		==Cryo-EM structure of Dengue virus serotype 1 strain WestPac 74 in complex with human antibody 1C19 Fab at 37 deg C (Class 1 particle)==
	<StructureSection load='7dwt' size='340' side='right'caption='[[7dwt]]' scene=''>		<StructureSection load='7dwt' size='340' side='right'caption='[[7dwt]], [[Resolution\|resolution]] 19.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7dwt]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_1 Dengue virus 1] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DWT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DWT FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwt OCA], [https://pdbe.org/7dwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwt RCSB], [https://www.ebi.ac.uk/pdbsum/7dwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwt ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 19Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwt OCA], [https://pdbe.org/7dwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwt RCSB], [https://www.ebi.ac.uk/pdbsum/7dwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwt ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/POLG_DEN1W POLG_DEN1W] Capsid protein C self-assembles to form an icosahedral capsid about 30 nm in diameter. The capsid encapsulates the genomic RNA (By similarity). prM acts as a chaperone for envelope protein E during intracellular virion assembly by masking and inactivating envelope protein E fusion peptide. prM is matured in the last step of virion assembly, presumably to avoid catastrophic activation of the viral fusion peptide induced by the acidic pH of the trans-Golgi network. After cleavage by host furin, the pr peptide is released in the extracellular medium and small envelope protein M and envelope protein E homodimers are dissociated (By similarity). Envelope protein E binding to host cell surface receptor is followed by virus internalization through clathrin-mediated endocytosis. Envelope protein E is subsequently involved in membrane fusion between virion and host late endosomes. Synthesized as a homodimer with prM which acts as a chaperone for envelope protein E. After cleavage of prM, envelope protein E dissociate from small envelope protein M and homodimerizes (By similarity). Non-structural protein 1 is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome (By similarity). Non-structural protein 2A may be involved viral RNA replication and capsid assembly (Potential). Non-structural protein 2B is a required cofactor for the serine protease function of NS3 (By similarity). Serine protease NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase. NS3 serine protease, in association with NS2B, performs its autocleavage and cleaves the polyprotein at dibasic sites in the cytoplasm: C-prM, NS2A-NS2B, NS2B-NS3, NS3-NS4A, NS4A-2K and NS4B-NS5. NS3 RNA helicase binds RNA and unwinds dsRNA in the 3' to 5' direction (By similarity). Non-structural protein 4A induces host endoplasmic reticulum membrane rearrangements leading to the formation of virus-induced membranous vesicles hosting the dsRNA and polymerase, functioning as a replication complex. NS4A might also regulate the ATPase activity of the NS3 helicase (By similarity). Peptide 2k functions as a signal peptide for NS4B and is required for the interferon antagonism activity of the latter (By similarity). Non-structural protein 4B inhibits interferon (IFN)-induced host STAT1 phosphorylation and nuclear translocation, thereby preventing the establishment of cellular antiviral state by blocking the IFN-alpha/beta pathway (By similarity). RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome, and performs the capping of genomes in the cytoplasm. NS5 methylates viral RNA cap at guanine N-7 and ribose 2'-O positions. Besides its role in genome replication, also prevents the establishment of cellular antiviral state by blocking the interferon-alpha/beta (IFN-alpha/beta) signaling pathway. Inhibits host TYK2 and STAT2 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway (By similarity).

			==See Also==
			*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Dengue virus 1]]
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Fibriansah G]]
			[[Category: Kostyuchenko VA]]
			[[Category: Lok SM]]
			[[Category: Ng TS]]
			[[Category: Shi J]]

OCA at 10:02, 17 February 2021

2021-02-17T10:02:08Z

← Older revision		Revision as of 10:02, 17 February 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7dwt is ~~ON HOLD~~		====
			<StructureSection load='7dwt' size='340' side='right'caption='[[7dwt]]' scene=''>
	~~Authors:~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dwt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dwt OCA], [https://pdbe.org/7dwt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dwt RCSB], [https://www.ebi.ac.uk/pdbsum/7dwt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dwt ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA: Protected "7dwt" [edit=sysop:move=sysop]

2021-01-27T06:39:38Z

Protected "7dwt" [edit=sysop:move=sysop]

← Older revision	Revision as of 06:39, 27 January 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7dwt is ON HOLD Authors: Description: Category: Unreleased Structures

2021-01-27T06:39:37Z

New page: '''Unreleased structure''' The entry 7dwt is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7dwt is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7dwt - Revision history

OCA at 10:54, 27 March 2024

OCA at 10:02, 17 February 2021

OCA: Protected "7dwt" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7dwt is ON HOLD Authors: Description: Category: Unreleased Structures