OCA at 16:49, 29 November 2023

2023-11-29T16:49:20Z

← Older revision		Revision as of 16:49, 29 November 2023
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	==crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain==		==crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain==
	<StructureSection load='7ea2' size='340' side='right'caption='[[7ea2]]' scene=''>		<StructureSection load='7ea2' size='340' side='right'caption='[[7ea2]], [[Resolution\|resolution]] 2.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EA2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ea2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EA2 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea2 OCA], [https://pdbe.org/7ea2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea2 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.14Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea2 OCA], [https://pdbe.org/7ea2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea2 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/RBCC1_HUMAN RBCC1_HUMAN] Involved in autophagy (PubMed:21775823). Regulates early events but also late events of autophagosome formation through direct interaction with Atg16L1 (PubMed:23392225). Required for the formation of the autophagosome-like double-membrane structure that surrounds the Salmonella-containing vacuole (SCV) during S.typhimurium infection and subsequent xenophagy (By similarity). Involved in repair of DNA damage caused by ionizing radiation, which subsequently improves cell survival by decreasing apoptosis (By similarity). Inhibits PTK2/FAK1 and PTK2B/PYK2 kinase activity, affecting their downstream signaling pathways (PubMed:10769033, PubMed:12221124). Plays a role as a modulator of TGF-beta-signaling by restricting substrate specificity of RNF111 (By similarity). Functions as a DNA-binding transcription factor (PubMed:12095676). Is a potent regulator of the RB1 pathway through induction of RB1 expression (PubMed:14533007). Plays a crucial role in muscular differentiation (PubMed:12163359). Plays an indispensable role in fetal hematopoiesis and in the regulation of neuronal homeostasis (By similarity).[UniProtKB:Q9ESK9]<ref>PMID:10769033</ref> <ref>PMID:12095676</ref> <ref>PMID:12163359</ref> <ref>PMID:12221124</ref> <ref>PMID:14533007</ref> <ref>PMID:21775823</ref> <ref>PMID:23392225</ref> [https://www.uniprot.org/uniprot/AZI2_HUMAN AZI2_HUMAN] Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.<ref>PMID:14560022</ref> <ref>PMID:15611223</ref> <ref>PMID:21931631</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The recruitment of Unc-51-like kinase and TANK-binding kinase 1 complexes is essential for Nuclear dot protein 52-mediated selective autophagy and relies on the specific association of NDP52, RB1-inducible coiled-coil protein 1, and Nak-associated protein 1 (5-azacytidine-induced protein 2, AZI2). However, the underlying molecular mechanism remains elusive. Here, we find that except for the NDP52 SKIP carboxyl homology (SKICH)/RB1CC1 coiled-coil interaction, the LC3-interacting region of NDP52 can directly interact with the RB1CC1 Claw domain, as that of NAP1 FIP200-binding region (FIR). The determined crystal structures of NDP52 SKICH/RB1CC1 complex, NAP1 FIR/RB1CC1 complex, and the related NAP1 FIR/Gamma-aminobutyric acid receptor-associated protein complex not only elucidate the molecular bases underpinning the interactions of RB1CC1 with NDP52 and NAP1 but also reveal that RB1CC1 Claw and Autophagy-related protein 8 family proteins are competitive in binding to NAP1 and NDP52. Overall, our findings provide mechanistic insights into the interactions of NDP52, NAP1 with RB1CC1 and ATG8 family proteins.

			Structural and biochemical advances on the recruitment of the autophagy-initiating ULK and TBK1 complexes by autophagy receptor NDP52.,Fu T, Zhang M, Zhou Z, Wu P, Peng C, Wang Y, Gong X, Li Y, Wang Y, Xu X, Li M, Shen L, Pan L Sci Adv. 2021 Aug 13;7(33). pii: 7/33/eabi6582. doi: 10.1126/sciadv.abi6582., Print 2021 Aug. PMID:34389544<ref>PMID:34389544</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ea2" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Fu T]]		[[Category: Fu T]]
	[[Category: Pan L]]		[[Category: Pan L]]

OCA at 15:25, 22 December 2021

2021-12-22T15:25:04Z

← Older revision		Revision as of 15:25, 22 December 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ea2 is ~~ON HOLD~~		==crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain==
			<StructureSection load='7ea2' size='340' side='right'caption='[[7ea2]]' scene=''>
	~~Authors~~: Fu, T., ~~Pan~~, L.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EA2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EA2 FirstGlance]. <br>
	~~Description: crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea2 OCA], [https://pdbe.org/7ea2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea2 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea2 ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Pan, L~~]]		__TOC__
	[[Category: ~~Fu, T~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Fu T]]
			[[Category: Pan L]]

OCA: Protected "7ea2" [edit=sysop:move=sysop]

2021-03-17T05:16:46Z

Protected "7ea2" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:16, 17 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ea2 is ON HOLD Authors: Fu, T., Pan, L. Description: crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain [[Category: Unreleased Struc...

2021-03-17T05:16:45Z

New page: '''Unreleased structure''' The entry 7ea2 is ON HOLD Authors: Fu, T., Pan, L. Description: crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 7ea2 is ON HOLD

Authors: Fu, T., Pan, L.

Description: crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain
[[Category: Unreleased Structures]]
[[Category: Pan, L]]
[[Category: Fu, T]]

7ea2 - Revision history

OCA at 16:49, 29 November 2023

OCA at 15:25, 22 December 2021

OCA: Protected "7ea2" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ea2 is ON HOLD Authors: Fu, T., Pan, L. Description: crystal structure of NAP1 FIR in complex with RB1CC1 Claw domain [[Category: Unreleased Struc...