7ea3 - Revision history

OCA at 05:43, 5 June 2024

2024-06-05T05:43:25Z

← Older revision		Revision as of 05:43, 5 June 2024
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	====		==Intact Ypt32-TRAPPII (dimer).==
	<StructureSection load='7ea3' size='340' side='right'caption='[[7ea3]]' scene=''>		<StructureSection load='7ea3' size='340' side='right'caption='[[7ea3]], [[Resolution\|resolution]] 4.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ea3]] is a 22 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EA3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EA3 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea3 OCA], [https://pdbe.org/7ea3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea3 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.31Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea3 OCA], [https://pdbe.org/7ea3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea3 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/BET3_YEAST BET3_YEAST] Component of the TRAPP I, TRAPP II and TRAPP III complexes which act as guanine nucleotide exchange factors (GEF) for YPT1. TRAPP I plays a key role in the late stages of endoplasmic reticulum to Golgi traffic. TRAPP II plays a role in intra-Golgi transport. TRAPP III plays a role in autophagosome formation. Required for sporulation. Has a role late in meiosis following DNA replication.<ref>PMID:8590804</ref> <ref>PMID:9564032</ref> <ref>PMID:11239471</ref> <ref>PMID:20972447</ref> <ref>PMID:20375281</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Transport protein particle (TRAPP) complexes belong to the multiprotein tethering complex and exist in three forms-core TRAPP/TRAPPI, TRAPPII, and TRAPPIII. TRAPPII activates GTPase Ypt31/Ypt32 as the guanine nucleotide exchange factor in the trans-Golgi network to determine the maturation of Golgi cisternae into post-Golgi carriers in yeast. Here, we present cryo-EM structures of yeast TRAPPII in apo and Ypt32-bound states. All the structures show a dimeric architecture assembled by two triangle-shaped monomers, while the monomer in the apo state exhibits both open and closed conformations, and the monomer in the Ypt32-bound form only captures the closed conformation. Located in the interior of the monomer, Ypt32 binds with both core TRAPP/TRAPPI and Trs120 via its nucleotide-binding domain and binds with Trs31 via its hypervariable domain. Combined with functional analysis, the structures provide insights into the assembly of TRAPPII and the mechanism of the specific activation of Ypt31/Ypt32 by TRAPPII.

			Structural basis for assembly of TRAPPII complex and specific activation of GTPase Ypt31/32.,Mi C, Zhang L, Huang G, Shao G, Yang F, You X, Dong MQ, Sun S, Sui SF Sci Adv. 2022 Jan 28;8(4):eabi5603. doi: 10.1126/sciadv.abi5603. Epub 2022 Jan , 26. PMID:35080977<ref>PMID:35080977</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ea3" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[GTP-binding protein 3D structures\|GTP-binding protein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Z-disk~~]]		[[Category: Saccharomyces cerevisiae S288C]]
			[[Category: Mi CC]]
			[[Category: Sui SF]]

OCA at 07:21, 9 February 2022

2022-02-09T07:21:57Z

← Older revision		Revision as of 07:21, 9 February 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ea3 ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7ea3' size='340' side='right'caption='[[7ea3]]' scene=''>
	~~Authors~~: ~~Mi, C~~.C.~~, Sui, S~~.F.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Intact Ypt32~~-~~TRAPPII (dimer)~~.		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ea3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ea3 OCA], [https://pdbe.org/7ea3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ea3 RCSB], [https://www.ebi.ac.uk/pdbsum/7ea3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ea3 ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[Category: ~~Mi, C.C~~]]		__TOC__
	[[Category: ~~Sui, S.F~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Z-disk]]

OCA at 05:25, 22 September 2021

2021-09-22T05:25:55Z

← Older revision		Revision as of 05:25, 22 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7ea3 is ON HOLD		The entry 7ea3 is ON HOLD until Paper Publication

	Authors: Mi, C.C., Sui, S.F.		Authors: Mi, C.C., Sui, S.F.

OCA at 08:38, 14 July 2021

2021-07-14T08:38:10Z

← Older revision		Revision as of 08:38, 14 July 2021
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	Authors: Mi, C.C., Sui, S.F.		Authors: Mi, C.C., Sui, S.F.

	Description: Intact of Ypt32-TRAPPII dimer.		Description: Intact Ypt32-TRAPPII (dimer).
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Mi, C.C]]		[[Category: Mi, C.C]]
	[[Category: Sui, S.F]]		[[Category: Sui, S.F]]

OCA: Protected "7ea3" [edit=sysop:move=sysop]

2021-03-17T05:16:49Z

Protected "7ea3" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:16, 17 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ea3 is ON HOLD Authors: Mi, C.C., Sui, S.F. Description: Intact of Ypt32-TRAPPII dimer. Category: Unreleased Structures Category: Mi, C.C ...

2021-03-17T05:16:48Z

New page: '''Unreleased structure''' The entry 7ea3 is ON HOLD Authors: Mi, C.C., Sui, S.F. Description: Intact of Ypt32-TRAPPII dimer. Category: Unreleased Structures Category: Mi, C.C ...

New page

'''Unreleased structure'''

The entry 7ea3 is ON HOLD

Authors: Mi, C.C., Sui, S.F.

Description: Intact of Ypt32-TRAPPII dimer.
[[Category: Unreleased Structures]]
[[Category: Mi, C.C]]
[[Category: Sui, S.F]]