7ebf - Revision history

OCA at 06:35, 25 June 2025

2025-06-25T06:35:06Z

← Older revision		Revision as of 06:35, 25 June 2025
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	<StructureSection load='7ebf' size='340' side='right'caption='[[7ebf]], [[Resolution\|resolution]] 2.63Å' scene=''>		<StructureSection load='7ebf' size='340' side='right'caption='[[7ebf]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7ebf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EBF FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EBF FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.63Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.63Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ebf OCA], [https://pdbe.org/7ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ebf RCSB], [https://www.ebi.ac.uk/pdbsum/7ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ebf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ebf OCA], [https://pdbe.org/7ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ebf RCSB], [https://www.ebi.ac.uk/pdbsum/7ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ebf ProSAT]</span></td></tr>
	</table>		</table>
	== ~~Function~~ ==		<div style="background-color:#fffaf0;">
	~~[https://www~~.~~uniprot~~.~~org/uniprot/ACEA_CANAX ACEA_CANAX] Catalyzes~~ the ~~formation~~ of ~~succinate~~ and ~~glyoxylate from isocitrate~~, a key ~~step~~ of the ~~glyoxylate cycle, which operates~~ as ~~an anaplerotic route~~ for ~~replenishing~~ the ~~tricarboxylic acid cycle~~. ~~Required~~ for ~~growth on ethanol or acetate~~, ~~but dispensable when fermentable carbon sources are available~~. ~~Acts also on 2~~-~~methylisocitrate.[UniProtKB~~:~~P28240]~~		== Publication Abstract from PubMed ==
			In Saccharomyces cerevisiae, the glyoxylate cycle is controlled through the posttranslational regulation of its component enzymes, such as isocitrate lyase (ICL), which catalyzes the first unique step of the cycle. The ICL of S.cerevisiae (ScIcl1) is tagged for proteasomal degradation through ubiquitination by a multisubunit ubiquitin ligase (the glucose-induced degradation-deficient (GID) complex), whereas that of the pathogenic yeast Candida albicans (CaIcl1) escapes this process. However, the reason for the ubiquitin targeting specificity of the GID complex for ScIcl1 and not for CaIcl1 is unclear. To gain some insight into this, in this study, the crystal structures of apo ScIcl1 and CaIcl1 in complex with formate and the cryogenic electron microscopy structure of apo CaIcl1 were determined at a resolution of 2.3, 2.7, and 2.6 A, respectively. A comparison of the various structures suggests that the orientation of N-terminal helix alpha1 in S.cerevisiae is likely key to repositioning of ubiquitination sites and contributes to the distinction found in C. albicans ubiquitin evasion mechanism. This finding gives us a better understanding of the molecular mechanism of ubiquitin-dependent ScIcl1 degradation and could serve as a theoretical basis for the research and development of anti-C. albicans drugs based on the concept of CaIcl1 ubiquitination.

			Structural insights into the targeting specificity of ubiquitin ligase for S. cerevisiae isocitrate lyase but not C. albicans isocitrate lyase.,Hiragi K, Nishio K, Moriyama S, Hamaguchi T, Mizoguchi A, Yonekura K, Tani K, Mizushima T J Struct Biol. 2021 May 24;213(3):107748. doi: 10.1016/j.jsb.2021.107748. PMID:34033899<ref>PMID:34033899</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7ebf" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Candida albicans]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hamaguchi T]]		[[Category: Hamaguchi T]]

OCA at 10:54, 27 March 2024

2024-03-27T10:54:32Z

← Older revision		Revision as of 10:54, 27 March 2024
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	==Cryo-EM structure of Isocitrate lyase-1 from Candida albicans==		==Cryo-EM structure of Isocitrate lyase-1 from Candida albicans==
	<StructureSection load='7ebf' size='340' side='right'caption='[[7ebf]]' scene=''>		<StructureSection load='7ebf' size='340' side='right'caption='[[7ebf]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EBF FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ebf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EBF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ebf OCA], [https://pdbe.org/7ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ebf RCSB], [https://www.ebi.ac.uk/pdbsum/7ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ebf ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.63Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ebf OCA], [https://pdbe.org/7ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ebf RCSB], [https://www.ebi.ac.uk/pdbsum/7ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ebf ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/ACEA_CANAX ACEA_CANAX] Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.[UniProtKB:P28240]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Candida albicans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Hamaguchi T]]		[[Category: Hamaguchi T]]

OCA at 07:31, 25 June 2021

2021-06-25T07:31:08Z

← Older revision		Revision as of 07:31, 25 June 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7ebf is ~~ON HOLD~~		==Cryo-EM structure of Isocitrate lyase-1 from Candida albicans==
			<StructureSection load='7ebf' size='340' side='right'caption='[[7ebf]]' scene=''>
	~~Authors~~: ~~Hiragi~~, K., ~~Nishio~~, K.~~, Moriyama, S~~., ~~Hamaguchi, T~~.~~, Mizoguchi, A~~.~~, Yonekura, K~~., ~~Tani, K~~.~~, Mizushima, T~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EBF FirstGlance]. <br>
	~~Description: Cryo-EM structure of Isocitrate lyase-1 from Candida albicans~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ebf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ebf OCA], [https://pdbe.org/7ebf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ebf RCSB], [https://www.ebi.ac.uk/pdbsum/7ebf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ebf ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Yonekura, K~~]]		__TOC__
	[[Category: ~~Hamaguchi, T~~]]		</StructureSection>
	[[Category: ~~Moriyama, S~~]]		[[Category: Large Structures]]
	[[Category: ~~Mizoguchi, A~~]]		[[Category: Hamaguchi T]]
	[[Category: ~~Mizushima, T~~]]		[[Category: Hiragi K]]
	[[Category: ~~Hiragi,~~ K]]		[[Category: Mizoguchi A]]
	[[Category: ~~Nishio,~~ K]]		[[Category: Mizushima T]]
	[[Category: ~~Tani,~~ K]]		[[Category: Moriyama S]]
			[[Category: Nishio K]]
			[[Category: Tani K]]
			[[Category: Yonekura K]]

OCA at 10:55, 9 June 2021

2021-06-09T10:55:09Z

← Older revision		Revision as of 10:55, 9 June 2021
Line 5:		Line 5:
	Authors: Hiragi, K., Nishio, K., Moriyama, S., Hamaguchi, T., Mizoguchi, A., Yonekura, K., Tani, K., Mizushima, T.		Authors: Hiragi, K., Nishio, K., Moriyama, S., Hamaguchi, T., Mizoguchi, A., Yonekura, K., Tani, K., Mizushima, T.

	Description: Cryo-EM structure of Isocitrate lyase ~~Icl1~~ from Candida albicans		Description: Cryo-EM structure of Isocitrate lyase-1 from Candida albicans
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Yonekura, K]]		[[Category: Yonekura, K]]

OCA: Protected "7ebf" [edit=sysop:move=sysop]

2021-03-17T05:19:11Z

Protected "7ebf" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:19, 17 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7ebf is ON HOLD Authors: Hiragi, K., Nishio, K., Moriyama, S., Hamaguchi, T., Mizoguchi, A., Yonekura, K., Tani, K., Mizushima, T. Description: Cry...

2021-03-17T05:19:10Z

New page: '''Unreleased structure''' The entry 7ebf is ON HOLD Authors: Hiragi, K., Nishio, K., Moriyama, S., Hamaguchi, T., Mizoguchi, A., Yonekura, K., Tani, K., Mizushima, T. Description: Cry...

New page

'''Unreleased structure'''

The entry 7ebf is ON HOLD

Authors: Hiragi, K., Nishio, K., Moriyama, S., Hamaguchi, T., Mizoguchi, A., Yonekura, K., Tani, K., Mizushima, T.

Description: Cryo-EM structure of Isocitrate lyase Icl1 from Candida albicans
[[Category: Unreleased Structures]]
[[Category: Yonekura, K]]
[[Category: Hamaguchi, T]]
[[Category: Moriyama, S]]
[[Category: Mizoguchi, A]]
[[Category: Mizushima, T]]
[[Category: Hiragi, K]]
[[Category: Nishio, K]]
[[Category: Tani, K]]