7eee - Revision history

OCA at 13:33, 6 November 2024

2024-11-06T13:33:12Z

← Older revision		Revision as of 13:33, 6 November 2024
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	<StructureSection load='7eee' size='340' side='right'caption='[[7eee]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='7eee' size='340' side='right'caption='[[7eee]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>~~[[7eee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_sp. Chaetomium sp.].~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>		<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>
	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.660792Å</td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.660792Å</td></tr>
	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	~~[[Category: Chaetomium sp]]~~
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jiang ZQ]]		[[Category: Jiang ZQ]]
	[[Category: Ma JW]]		[[Category: Ma JW]]

OCA at 16:51, 29 November 2023

2023-11-29T16:51:29Z

← Older revision		Revision as of 16:51, 29 November 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7eee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_sp. Chaetomium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_sp. Chaetomium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.660792Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>
	</table>		</table>

			==See Also==
			*[[Glucanase 3D structures\|Glucanase 3D structures]]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 06:20, 8 September 2022

2022-09-08T06:20:46Z

← Older revision		Revision as of 06:20, 8 September 2022
Line 3:		Line 3:
	<StructureSection load='7eee' size='340' side='right'caption='[[7eee]], [[Resolution\|resolution]] 1.66Å' scene=''>		<StructureSection load='7eee' size='340' side='right'caption='[[7eee]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7eee]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eee]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_sp. Chaetomium sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>
	</table>		</table>
	~~<div style="background-color:#fffaf0;">~~
	~~== Publication Abstract from PubMed ==~~
	beta-1,3-1,4-Glucanases are a type of hydrolytic enzymes capable of catalyzing the strict cleavage of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans and have exhibited great potential in food and feed industrials. In this study, a novel glycoside hydrolase (GH) family 12 beta-1,3-1,4-glucanase (CtGlu12A) from the thermophilic fungus Chaetomium sp. CQ31 was identified and biochemically characterized. CtGlu12A was most active at pH 7.5 and 65 degrees C, respectively, and exhibited a high specific activity of 999.9 U mg(-1) towards lichenin. It maintained more than 80% of its initial activity in a wide pH range of 5.0-11.0, and up to 60 degrees C after incubation at 55 degrees C for 60 min. Moreover, the crystal structures of CtGlu12A with gentiobiose and tetrasccharide were resolved. CtGlu12A had a beta-jellyroll fold, and performed retaining mechanism with two glutamic acids severing as the catalytic residues. In the complex structure, cellobiose molecule showed two binding modes, occupying subsites -2 to -1 and subsites + 1 to + 2, respectively. The concave cleft made mixed beta-1,3-1,4-glucan substrates maintain a bent conformation to fit into the active site. Overall, this study is not only helpful for the understanding of the substrate-binding model and catalytic mechanism of GH 12 beta-1,3-1,4-glucanases, but also provides a basis for further enzymatic engineering of beta-1,3-1,4-glucanases.

	Structural and biochemical insights into the substrate-binding mechanism of a glycoside hydrolase family 12 beta-1,3-1,4-glucanase from Chaetomium sp.,Ma J, Li Y, Han S, Jiang Z, Yan Q, Yang S J Struct Biol. 2021 Sep;213(3):107774. doi: 10.1016/j.jsb.2021.107774. Epub 2021 , Jul 27. PMID:34329700<ref>PMID:34329700</ref>

	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~
	~~</div>~~
	~~<div class="pdbe-citations 7eee" style="background-color:#fffaf0;"></div>~~
	~~== References ==~~
	~~<references/>~~
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Chaetomium sp]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Licheninase]]~~		[[Category: Jiang ZQ]]
	[[Category: Jiang~~, Z Q~~]]		[[Category: Ma JW]]
	[[Category: Ma~~, J W]]~~
	~~[[Category: Catalytic mechanism]]~~
	~~[[Category: Glucanase]]~~
	~~[[Category: Hydrolase]]~~
	~~[[Category: Structural protein]]~~
	~~[[Category: Substrate binding~~]]

OCA at 05:22, 13 July 2022

2022-07-13T05:22:52Z

← Older revision		Revision as of 05:22, 13 July 2022
Line 1:		Line 1:

	==Complex structure of glycoside hydrolase family 12 beta-1,3-1,4-glucanase with gentiobiose==		==Complex structure of glycoside hydrolase family 12 beta-1,3-1,4-glucanase with gentiobiose==
	<StructureSection load='7eee' size='340' side='right'caption='[[7eee]]' scene=''>		<StructureSection load='7eee' size='340' side='right'caption='[[7eee]], [[Resolution\|resolution]] 1.66Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eee]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Licheninase Licheninase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.73 3.2.1.73] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			beta-1,3-1,4-Glucanases are a type of hydrolytic enzymes capable of catalyzing the strict cleavage of beta-1,4 glycosidic bonds adjacent to beta-1,3 linkages in beta-D-glucans and have exhibited great potential in food and feed industrials. In this study, a novel glycoside hydrolase (GH) family 12 beta-1,3-1,4-glucanase (CtGlu12A) from the thermophilic fungus Chaetomium sp. CQ31 was identified and biochemically characterized. CtGlu12A was most active at pH 7.5 and 65 degrees C, respectively, and exhibited a high specific activity of 999.9 U mg(-1) towards lichenin. It maintained more than 80% of its initial activity in a wide pH range of 5.0-11.0, and up to 60 degrees C after incubation at 55 degrees C for 60 min. Moreover, the crystal structures of CtGlu12A with gentiobiose and tetrasccharide were resolved. CtGlu12A had a beta-jellyroll fold, and performed retaining mechanism with two glutamic acids severing as the catalytic residues. In the complex structure, cellobiose molecule showed two binding modes, occupying subsites -2 to -1 and subsites + 1 to + 2, respectively. The concave cleft made mixed beta-1,3-1,4-glucan substrates maintain a bent conformation to fit into the active site. Overall, this study is not only helpful for the understanding of the substrate-binding model and catalytic mechanism of GH 12 beta-1,3-1,4-glucanases, but also provides a basis for further enzymatic engineering of beta-1,3-1,4-glucanases.

			Structural and biochemical insights into the substrate-binding mechanism of a glycoside hydrolase family 12 beta-1,3-1,4-glucanase from Chaetomium sp.,Ma J, Li Y, Han S, Jiang Z, Yan Q, Yang S J Struct Biol. 2021 Sep;213(3):107774. doi: 10.1016/j.jsb.2021.107774. Epub 2021 , Jul 27. PMID:34329700<ref>PMID:34329700</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7eee" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Jiang ZQ]]		[[Category: Licheninase]]
	[[Category: Ma JW]]		[[Category: Jiang, Z Q]]
			[[Category: Ma, J W]]
			[[Category: Catalytic mechanism]]
			[[Category: Glucanase]]
			[[Category: Hydrolase]]
			[[Category: Structural protein]]
			[[Category: Substrate binding]]

OCA at 11:15, 23 March 2022

2022-03-23T11:15:57Z

← Older revision		Revision as of 11:15, 23 March 2022
Line 1:		Line 1:
	~~'''Unreleased structure'''~~

	~~The entry~~ 7eee is ~~ON HOLD until Paper Publication~~		==Complex structure of glycoside hydrolase family 12 beta-1,3-1,4-glucanase with gentiobiose==
			<StructureSection load='7eee' size='340' side='right'caption='[[7eee]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEE FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eee FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eee OCA], [https://pdbe.org/7eee PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eee RCSB], [https://www.ebi.ac.uk/pdbsum/7eee PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eee ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Jiang ZQ]]
			[[Category: Ma JW]]

OCA at 05:33, 14 April 2021

2021-04-14T05:33:56Z

← Older revision		Revision as of 05:33, 14 April 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7eee is ON HOLD		The entry 7eee is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7eee" [edit=sysop:move=sysop]

2021-03-24T05:26:05Z

Protected "7eee" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:26, 24 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7eee is ON HOLD Authors: Description: Category: Unreleased Structures

2021-03-24T05:26:04Z

New page: '''Unreleased structure''' The entry 7eee is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7eee is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]