7eef - Revision history

OCA at 16:51, 29 November 2023

2023-11-29T16:51:31Z

← Older revision		Revision as of 16:51, 29 November 2023
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	<StructureSection load='7eef' size='340' side='right'caption='[[7eef]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='7eef' size='340' side='right'caption='[[7eef]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7eef]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eef]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEF FirstGlance]. <br>
	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>EPHA7, EHK3, HEK11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
	~~<tr~~ id~~='activity'><td class="sblockLbl"><b>Activity:</b></td><td class~~="~~sblockDat~~">~~<span class='plainlinks'>[https://en.wikipedia.org/wiki/Receptor_protein~~-~~tyrosine_kinase Receptor protein-tyrosine kinase]~~, ~~with EC number~~ [~~https://www.brenda-enzymes.info/php/result_flat.php4?ecno=~~2.~~7.10.1 2.7.10.1] </span>~~</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eef OCA], [https://pdbe.org/7eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eef RCSB], [https://www.ebi.ac.uk/pdbsum/7eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eef ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eef OCA], [https://pdbe.org/7eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eef RCSB], [https://www.ebi.ac.uk/pdbsum/7eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eef ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/EPHA7_HUMAN EPHA7_HUMAN]] Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.<ref>PMID:17726105</ref>		[https://www.uniprot.org/uniprot/EPHA7_HUMAN EPHA7_HUMAN] Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.<ref>PMID:17726105</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7eef" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7eef" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Ephrin receptor 3D structures\|Ephrin receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: ~~Human~~]]		[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	~~[[Category: Receptor protein-tyrosine kinase]]~~		[[Category: Chakraborty S]]
	[[Category: Chakraborty, S]]		[[Category: Varma AK]]
	[[Category: Varma~~, A K]]~~
	~~[[Category: Kinase domain]]~~
	~~[[Category: Membrane protein]]~~
	~~[[Category: Mutant~~]]

OCA at 10:06, 14 July 2021

2021-07-14T10:06:51Z

← Older revision		Revision as of 10:06, 14 July 2021
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	==Crystal structure of EphA7 mutant G656E==		==Crystal structure of EphA7 mutant G656E==
	<StructureSection load='7eef' size='340' side='right'caption='[[7eef]]' scene=''>		<StructureSection load='7eef' size='340' side='right'caption='[[7eef]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEF FirstGlance]. <br>		<table><tr><td colspan='2'>[[7eef]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEF FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eef OCA], [https://pdbe.org/7eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eef RCSB], [https://www.ebi.ac.uk/pdbsum/7eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eef ProSAT]</span></td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EPHA7, EHK3, HEK11 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
			<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Receptor_protein-tyrosine_kinase Receptor protein-tyrosine kinase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.10.1 2.7.10.1] </span></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eef OCA], [https://pdbe.org/7eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eef RCSB], [https://www.ebi.ac.uk/pdbsum/7eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eef ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/EPHA7_HUMAN EPHA7_HUMAN]] Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7.<ref>PMID:17726105</ref>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Erythropoietin producing hepatocellular (Eph) forms the largest family of receptor tyrosine kinases (RTK). As a family, Eph regulates physiological events such as cell-cell interaction, cell migration, and adhesion. The Kinase domain is the catalytic core of the Eph receptor and is highly conserved sequentially. EphA7 has been recently regarded as a cancer driver gene and comprises several clinically important mutations. Three of the EphA7 mutations Gly656Glu, Gly656Arg, and Asp751His, present in the kinase domain, are predicted to be highly pathogenic. Furthermore, Gly656Glu and Gly656Arg are reported to be hotspot mutations. Considering the importance of mutations, crystals structure of EphA7 Gly656Glu, Gly656Arg, and Asp751His mutants has been explored. Changes in folding pattern and intramolecular interactions were observed in mutant structures. Secondary structural changes were observed in the hinge region of EphA7 Gly656Arg and Asp751His structure, affecting the transition of kinase domain between open and closed conformations. EphA7 Asp751His mutant structure shows a distorted nucleotide-binding groove. Differences were observed in hydrogen bonding and hydrophobic interactions between the catalytic and highly conserved DFG motif in the EphA7 mutants, which may influence the catalytic activity of kinase domain.

			Crystal structure of clinically reported mutations Gly656Arg, Gly656Glu and Asp751His identified in the kinase domain of EphA7.,Chakraborty S, Varma AK Biochem Biophys Res Commun. 2021 Jun 26;568:62-67. doi:, 10.1016/j.bbrc.2021.06.048. PMID:34186436<ref>PMID:34186436</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7eef" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
			[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chakraborty S]]		[[Category: Receptor protein-tyrosine kinase]]
	[[Category: Varma AK]]		[[Category: Chakraborty, S]]
			[[Category: Varma, A K]]
			[[Category: Kinase domain]]
			[[Category: Membrane protein]]
			[[Category: Mutant]]

OCA at 09:56, 7 July 2021

2021-07-07T09:56:46Z

← Older revision		Revision as of 09:56, 7 July 2021
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7eef is ~~ON HOLD until Paper Publication~~		==Crystal structure of EphA7 mutant G656E==
			<StructureSection load='7eef' size='340' side='right'caption='[[7eef]]' scene=''>
	~~Authors~~: ~~Chakraborty~~, S., ~~Varma~~, A.K.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7EEF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7EEF FirstGlance]. <br>
	~~Description: Crystal structure of EphA7 mutant G656E~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7eef FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7eef OCA], [https://pdbe.org/7eef PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7eef RCSB], [https://www.ebi.ac.uk/pdbsum/7eef PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7eef ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: Chakraborty, S]]		__TOC__
	[[Category: Varma~~, A.K~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Chakraborty S]]
			[[Category: Varma AK]]

OCA at 09:15, 31 March 2021

2021-03-31T09:15:45Z

← Older revision		Revision as of 09:15, 31 March 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7eef is ON HOLD		The entry 7eef is ON HOLD until Paper Publication

	Authors: Chakraborty, S., Varma, A.K.		Authors: Chakraborty, S., Varma, A.K.

OCA: Protected "7eef" [edit=sysop:move=sysop]

2021-03-24T05:26:07Z

Protected "7eef" [edit=sysop:move=sysop]

← Older revision	Revision as of 05:26, 24 March 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7eef is ON HOLD Authors: Chakraborty, S., Varma, A.K. Description: Crystal structure of EphA7 mutant G656E Category: Unreleased Structures [[Ca...

2021-03-24T05:26:06Z

New page: '''Unreleased structure''' The entry 7eef is ON HOLD Authors: Chakraborty, S., Varma, A.K. Description: Crystal structure of EphA7 mutant G656E Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 7eef is ON HOLD

Authors: Chakraborty, S., Varma, A.K.

Description: Crystal structure of EphA7 mutant G656E
[[Category: Unreleased Structures]]
[[Category: Chakraborty, S]]
[[Category: Varma, A.K]]