7f2e - Revision history

OCA at 17:08, 29 November 2023

2023-11-29T17:08:19Z

← Older revision		Revision as of 17:08, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7f2e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2E FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f2e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2E FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.1Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2e OCA], [https://pdbe.org/7f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2e RCSB], [https://www.ebi.ac.uk/pdbsum/7f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2e ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2e OCA], [https://pdbe.org/7f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2e RCSB], [https://www.ebi.ac.uk/pdbsum/7f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2e ProSAT]</span></td></tr>
	</table>		</table>

OCA at 20:20, 16 November 2022

2022-11-16T20:20:13Z

← Older revision		Revision as of 20:20, 16 November 2022
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	==SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)==		==SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)==
	<StructureSection load='7f2e' size='340' side='right'caption='[[7f2e]]' scene=''>		<StructureSection load='7f2e' size='340' side='right'caption='[[7f2e]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2E FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f2e]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2E FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2e OCA], [https://pdbe.org/7f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2e RCSB], [https://www.ebi.ac.uk/pdbsum/7f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2e ProSAT]</span></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2e OCA], [https://pdbe.org/7f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2e RCSB], [https://www.ebi.ac.uk/pdbsum/7f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2e ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			The ongoing outbreak of COVID-19 caused by SARS-CoV-2 has resulted in a serious public health threat globally. Nucleocapsid protein is a major structural protein of SARS-CoV-2 that plays important roles in the viral RNA packing, replication, assembly, and infection. Here, we report two crystal structures of nucleocapsid protein C-terminal domain (CTD) at resolutions of 2.0 A and 3.1 A, respectively. These two structures, crystallized under different conditions, contain 2 and 12 CTDs in asymmetric unit, respectively. Interestingly, despite different crystal packing, both structures show a similar dimeric form as the smallest unit, consistent with its solution form measured by the size-exclusion chromatography, suggesting an important role of CTD in the dimerization of nucleocapsid proteins. By analyzing the surface charge distribution, we identified a stretch of positively charged residues between Lys257 and Arg262 that are involved in RNA-binding. Through screening a single-domain antibodies (sdAbs) library, we identified four sdAbs targeting different regions of nucleocapsid protein with high affinities that have future potential to be used in viral detection and therapeutic purposes.

			Crystal structures of the SARS-CoV-2 nucleocapsid protein C-terminal domain and development of nucleocapsid-targeting nanobodies.,Jia Z, Liu C, Chen Y, Jiang H, Wang Z, Yao J, Yang J, Zhu J, Zhang B, Yuchi Z FEBS J. 2022 Jul;289(13):3813-3825. doi: 10.1111/febs.16239. Epub 2021 Oct 30. PMID:34665939<ref>PMID:34665939</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7f2e" style="background-color:#fffaf0;"></div>

			==See Also==
			*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
			[[Category: Severe acute respiratory syndrome coronavirus 2]]
	[[Category: Jiang H]]		[[Category: Jiang H]]
	[[Category: Liu C]]		[[Category: Liu C]]

OCA at 16:28, 20 October 2021

2021-10-20T16:28:42Z

← Older revision		Revision as of 16:28, 20 October 2021
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	~~'''Unreleased structure'''~~

	~~The entry 7f2e is ON HOLD~~		==SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)==
			<StructureSection load='7f2e' size='340' side='right'caption='[[7f2e]]' scene=''>
	~~Authors: Liu, C., Jiang, H.~~		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F2E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F2E FirstGlance]. <br>
	~~Description:~~ SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f2e OCA], [https://pdbe.org/7f2e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f2e RCSB], [https://www.ebi.ac.uk/pdbsum/7f2e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f2e ProSAT]</span></td></tr>
	[[Category: ~~Unreleased~~ Structures]]		</table>
	[[Category: ~~Liu, C~~]]		__TOC__
	[[Category: ~~Jiang, H~~]]		</StructureSection>
			[[Category: Large Structures]]
			[[Category: Jiang H]]
			[[Category: Liu C]]

OCA: Protected "7f2e" [edit=sysop:move=sysop]

2021-06-17T13:34:22Z

Protected "7f2e" [edit=sysop:move=sysop]

← Older revision	Revision as of 13:34, 17 June 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7f2e is ON HOLD Authors: Liu, C., Jiang, H. Description: SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer) [[Category: Unreleased Struc...

2021-06-17T13:34:21Z

New page: '''Unreleased structure''' The entry 7f2e is ON HOLD Authors: Liu, C., Jiang, H. Description: SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer) [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 7f2e is ON HOLD

Authors: Liu, C., Jiang, H.

Description: SARS-CoV-2 nucleocapsid protein C-terminal domain (dodecamer)
[[Category: Unreleased Structures]]
[[Category: Liu, C]]
[[Category: Jiang, H]]