7f8a - Revision history

OCA at 17:11, 29 November 2023

2023-11-29T17:11:09Z

← Older revision		Revision as of 17:11, 29 November 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/Q79N53_MYCSM Q79N53_MYCSM]]		[https://www.uniprot.org/uniprot/Q79N53_MYCSM Q79N53_MYCSM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 04:38, 8 September 2022

2022-09-08T04:38:55Z

← Older revision		Revision as of 04:38, 8 September 2022
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	<StructureSection load='7f8a' size='340' side='right'caption='[[7f8a]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='7f8a' size='340' side='right'caption='[[7f8a]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7f8a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f8a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis Mycolicibacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[[https://www.uniprot.org/uniprot/Q79N53_MYCSM Q79N53_MYCSM]]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: ~~Goh, B C~~]]		[[Category: Mycolicibacterium smegmatis]]
	[[Category: ~~Lescar, J~~]]		[[Category: Goh BC]]
	[[Category: ~~Erythromycin resistance methyltransferase]]~~		[[Category: Lescar J]]
	~~[[Category: Methyltransferase]]~~
	~~[[Category: Sam]]~~
	~~[[Category: Transferase~~]]

OCA at 18:13, 27 July 2022

2022-07-27T18:13:08Z

← Older revision		Revision as of 18:13, 27 July 2022
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	==Crystal structure of rRNA methyltransferase Erm38==		==Crystal structure of rRNA methyltransferase Erm38==
	<StructureSection load='7f8a' size='340' side='right'caption='[[7f8a]]' scene=''>		<StructureSection load='7f8a' size='340' side='right'caption='[[7f8a]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>		<table><tr><td colspan='2'>[[7f8a]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>
	</table>		</table>
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Erythromycin resistance methyltransferases (Erms) confer resistance to macrolide, lincosamide, and streptogramin antibiotics in Gram-positive bacteria and mycobacteria. Although structural information for ErmAM, ErmC, and ErmE exists from Gram-positive bacteria, little is known about the Erms in mycobacteria, as there are limited biochemical data and no structures available. Here, we present crystal structures of Erm38 from Mycobacterium smegmatis in apoprotein and cofactor-bound forms. Based on structural analysis and mutagenesis, we identified several catalytically critical, positively charged residues at a putative RNA-binding site. We found that mutation of any of these sites is sufficient to abolish methylation activity, whereas the corresponding RNA-binding affinity of Erm38 remains unchanged. The methylation reaction thus appears to require a precise ensemble of amino acids to accurately position the RNA substrate, such that the target nucleotide can be methylated. In addition, we computationally constructed a model of Erm38 in complex with a 32-mer RNA substrate. This model shows the RNA substrate stably bound to Erm38 by a patch of positively charged residues. Furthermore, a pi-pi stacking interaction between a key aromatic residue of Erm38 and a target adenine of the RNA substrate forms a critical interaction needed for methylation. Taken together, these data provide valuable insights into Erm-RNA interactions, which will aid subsequent structure-based drug design efforts.

			Crystal structure and functional analysis of mycobacterial erythromycin resistance methyltransferase Erm38 reveals its RNA-binding site.,Goh BC, Xiang X, Lescar J, Dedon PC J Biol Chem. 2022 Feb;298(2):101571. doi: 10.1016/j.jbc.2022.101571. Epub 2022, Jan 8. PMID:35007529<ref>PMID:35007529</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7f8a" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Goh BC]]		[[Category: Goh, B C]]
	[[Category: Lescar J]]		[[Category: Lescar, J]]
			[[Category: Erythromycin resistance methyltransferase]]
			[[Category: Methyltransferase]]
			[[Category: Sam]]
			[[Category: Transferase]]

OCA at 09:56, 12 January 2022

2022-01-12T09:56:27Z

← Older revision		Revision as of 09:56, 12 January 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7f8a is ~~ON HOLD until Paper Publication~~		==Crystal structure of rRNA methyltransferase Erm38==
			<StructureSection load='7f8a' size='340' side='right'caption='[[7f8a]]' scene=''>
	~~Authors~~:		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7F8A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7F8A FirstGlance]. <br>
	~~Description~~:		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7f8a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7f8a OCA], [https://pdbe.org/7f8a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7f8a RCSB], [https://www.ebi.ac.uk/pdbsum/7f8a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7f8a ProSAT]</span></td></tr>
	[[Category: ~~Unreleased Structures~~]]		</table>
			__TOC__
			</StructureSection>
			[[Category: Large Structures]]
			[[Category: Goh BC]]
			[[Category: Lescar J]]

OCA at 07:34, 29 September 2021

2021-09-29T07:34:15Z

← Older revision		Revision as of 07:34, 29 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7f8a is ON HOLD		The entry 7f8a is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7f8a" [edit=sysop:move=sysop]

2021-07-07T08:42:51Z

Protected "7f8a" [edit=sysop:move=sysop]

← Older revision	Revision as of 08:42, 7 July 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7f8a is ON HOLD Authors: Description: Category: Unreleased Structures

2021-07-07T08:42:50Z

New page: '''Unreleased structure''' The entry 7f8a is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7f8a is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]