7fc0 - Revision history

OCA at 17:13, 29 November 2023

2023-11-29T17:13:19Z

← Older revision		Revision as of 17:13, 29 November 2023
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	====		==Reconstitution of MbnABC complex from Rugamonas rubra ATCC-43154 (GroupIII)==
	<StructureSection load='7fc0' size='340' side='right'caption='[[7fc0]]' scene=''>		<StructureSection load='7fc0' size='340' side='right'caption='[[7fc0]], [[Resolution\|resolution]] 2.64Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>		<table><tr><td colspan='2'>[[7fc0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Rugamonas_rubra Rugamonas rubra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7FC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7FC0 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fc0 OCA], [https://pdbe.org/7fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fc0 ProSAT]</span></td></tr>		</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.643Å</td></tr>
			<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr>
			<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fc0 OCA], [https://pdbe.org/7fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fc0 ProSAT]</span></td></tr>
	</table>		</table>
			== Function ==
			[https://www.uniprot.org/uniprot/A0A1I4IFL0_9BURK A0A1I4IFL0_9BURK]
			<div style="background-color:#fffaf0;">
			== Publication Abstract from PubMed ==
			Methanobactins (Mbns) are a family of copper-binding peptides involved in copper uptake by methanotrophs, and are potential therapeutic agents for treating diseases characterized by disordered copper accumulation. Mbns are produced via modification of MbnA precursor peptides at cysteine residues catalyzed by the core biosynthetic machinery containing MbnB, an iron-dependent enzyme, and MbnC. However, mechanistic details underlying the catalysis of the MbnBC holoenzyme remain unclear. Here, we present crystal structures of MbnABC complexes from two distinct species, revealing that the leader peptide of the substrate MbnA binds MbnC for recruitment of the MbnBC holoenzyme, while the core peptide of MbnA resides in the catalytic cavity created by the MbnB-MbnC interaction which harbors a unique tri-iron cluster. Ligation of the substrate sulfhydryl group to the tri-iron center achieves a dioxygen-dependent reaction for oxazolone-thioamide installation. Structural analysis of the MbnABC complexes together with functional investigation of MbnB variants identified a conserved catalytic aspartate residue as a general base required for MbnBC-mediated MbnA modification. Together, our study reveals the similar architecture and function of MbnBC complexes from different species, demonstrating an evolutionarily conserved catalytic mechanism of the MbnBC holoenzymes.

			Crystal structure and catalytic mechanism of the MbnBC holoenzyme required for methanobactin biosynthesis.,Dou C, Long Z, Li S, Zhou D, Jin Y, Zhang L, Zhang X, Zheng Y, Li L, Zhu X, Liu Z, He S, Yan W, Yang L, Xiong J, Fu X, Qi S, Ren H, Chen S, Dai L, Wang B, Cheng W Cell Res. 2022 Mar;32(3):302-314. doi: 10.1038/s41422-022-00620-2. Epub 2022 Feb , 2. PMID:35110668<ref>PMID:35110668</ref>

			From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
			</div>
			<div class="pdbe-citations 7fc0" style="background-color:#fffaf0;"></div>
			== References ==
			<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Z~~-disk~~]]		[[Category: Rugamonas rubra]]
			[[Category: Chao D]]
			[[Category: Dan Z]]
			[[Category: Li Z]]
			[[Category: Shoujie L]]
			[[Category: Wei C]]
			[[Category: Ying J]]
			[[Category: Zhaolin L]]

OCA at 06:13, 16 March 2022

2022-03-16T06:13:11Z

← Older revision		Revision as of 06:13, 16 March 2022
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	~~'''Unreleased structure'''~~

	~~The entry~~ 7fc0 ~~is ON HOLD until Paper Publication~~		====
			<StructureSection load='7fc0' size='340' side='right'caption='[[7fc0]]' scene=''>
	~~Authors~~: ~~Chao, D~~.~~, Zhaolin, L~~.~~, Shoujie, L~~.~~, Li, Z~~.~~, Dan, Z~~.~~, Ying, J~~.~~, Wei, C~~.		== Structural highlights ==
			<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
	~~Description~~: ~~Reconstitution of MbnABC complex from Rugamonas rubra ATCC-43154 (GroupIII)~~		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7fc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7fc0 OCA], [https://pdbe.org/7fc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7fc0 RCSB], [https://www.ebi.ac.uk/pdbsum/7fc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7fc0 ProSAT]</span></td></tr>
	[[~~Category~~: ~~Unreleased Structures~~]]		</table>
	[[~~Category~~: ~~Wei~~, ~~C]]~~		__TOC__
	[~~[Category~~: Li, ~~Z]]~~		</StructureSection>
	[~~[Category~~: ~~Ying~~, ~~J]]~~		[[Category: Large Structures]]
	[~~[Category~~: ~~Shoujie~~, ~~L]]~~		[[Category: Z-disk]]
	[~~[Category~~: ~~Chao, D]~~]
	[[Category: ~~Dan, Z~~]]
	[[Category: ~~Zhaolin, L~~]]

OCA at 05:26, 22 September 2021

2021-09-22T05:26:52Z

← Older revision		Revision as of 05:26, 22 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

	The entry 7fc0 is ON HOLD		The entry 7fc0 is ON HOLD until Paper Publication

	Authors: Chao, D., Zhaolin, L., Shoujie, L., Li, Z., Dan, Z., Ying, J.		Authors: Chao, D., Zhaolin, L., Shoujie, L., Li, Z., Dan, Z., Ying, J., Wei, C.

	Description: Reconstitution of MbnABC complex from GroupIII		Description: Reconstitution of MbnABC complex from Rugamonas rubra ATCC-43154 (GroupIII)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
			[[Category: Wei, C]]
	[[Category: Li, Z]]		[[Category: Li, Z]]
			[[Category: Ying, J]]
	[[Category: Shoujie, L]]		[[Category: Shoujie, L]]
	[[Category: Chao, D]]		[[Category: Chao, D]]
	~~[[Category: Ying, J]]~~
	[[Category: Dan, Z]]		[[Category: Dan, Z]]
	[[Category: Zhaolin, L]]		[[Category: Zhaolin, L]]

OCA: Protected "7fc0" [edit=sysop:move=sysop]

2021-07-21T07:48:02Z

Protected "7fc0" [edit=sysop:move=sysop]

← Older revision	Revision as of 07:48, 21 July 2021
(No difference)

OCA: New page: '''Unreleased structure''' The entry 7fc0 is ON HOLD Authors: Chao, D., Zhaolin, L., Shoujie, L., Li, Z., Dan, Z., Ying, J. Description: Reconstitution of MbnABC complex from GroupIII ...

2021-07-21T07:48:01Z

New page: '''Unreleased structure''' The entry 7fc0 is ON HOLD Authors: Chao, D., Zhaolin, L., Shoujie, L., Li, Z., Dan, Z., Ying, J. Description: Reconstitution of MbnABC complex from GroupIII ...

New page

'''Unreleased structure'''

The entry 7fc0 is ON HOLD

Authors: Chao, D., Zhaolin, L., Shoujie, L., Li, Z., Dan, Z., Ying, J.

Description: Reconstitution of MbnABC complex from GroupIII
[[Category: Unreleased Structures]]
[[Category: Li, Z]]
[[Category: Shoujie, L]]
[[Category: Chao, D]]
[[Category: Ying, J]]
[[Category: Dan, Z]]
[[Category: Zhaolin, L]]